Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,927,340	G→A	D345N (GAT→AAT)	ppnN →	nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,927,340	0	G	A	100.0%	274.5 / NA	76	D345N (GAT→AAT)	ppnN	nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/37);  total (39/37)

GACGAGTTTGTCGTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCGATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTGAAGTGCTGGCTGCCGACCTGCG  >  NC_000913/2927205‑2927473                                                                                                                                        |                                                                                                                                      gACGAGTTTGTCGTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGc                                                                                                                                   >  1:1243348/1‑140 (MQ=255) gACGAGTTTGTCGTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGc                                                                                                                                   >  1:257655/1‑140 (MQ=255)   cGAGTTTGTCGTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCt                                                                                                                                 <  1:1012432/140‑1 (MQ=255)   cGAGTTTGTCGTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCt                                                                                                                                 <  1:592392/140‑1 (MQ=255)         tGTCGTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCt                                                                                                                           >  2:21946/1‑140 (MQ=255)           tCGTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCt                                                                                                                                 <  1:859924/132‑1 (MQ=255)           tCGTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCt                                                                                                                                 >  2:859924/1‑132 (MQ=255)             gTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTaa                                                                                                                       <  1:1504631/140‑1 (MQ=255)                cATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTg                                                                                                                    >  1:443521/1‑140 (MQ=255)                 aTACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTgg                                                                                                                   <  2:192044/140‑1 (MQ=255)                     gCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCaa                                                                                                               >  1:374324/1‑140 (MQ=255)                         ggTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATgcg                                                                                                           >  1:127642/1‑140 (MQ=255)                         ggTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATgcg                                                                                                           >  1:1397440/1‑140 (MQ=255)                          gTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAAACGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATgcgc                                                                                                          >  1:1067610/1‑140 (MQ=255)                           tGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCa                                                                                                         <  2:374324/140‑1 (MQ=255)                               aaCGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTgc                                                                                                     >  1:1166175/1‑140 (MQ=255)                                  gcgcgcCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGcc                                                                                                  <  2:519709/140‑1 (MQ=255)                                   cgcgcCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCa                                                                                                 >  1:969253/1‑140 (MQ=255)                                   cgcgcCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCa                                                                                                 >  2:174843/1‑140 (MQ=255)                                   cgcgcCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCa                                                                                                 >  2:680389/1‑140 (MQ=255)                                   cgcgcCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCa                                                                                                 >  2:157803/1‑140 (MQ=255)                                                    atcatcatTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGtt                                                                                >  2:337987/1‑140 (MQ=255)                                                     tcatcatTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGttt                                                                               >  2:694525/1‑140 (MQ=255)                                                      catcatTGATGACGCCGCTGAAGTCGCTCGTAAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGAtt                                                                                             >  1:395084/1‑125 (MQ=255)                                                      catcatTGATGACGCCGCTGAAGTCGCTCGTAAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGAtt                                                                                             <  2:395084/125‑1 (MQ=255)                                                       atcatTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGa                                                                             <  2:1186042/140‑1 (MQ=255)                                                       atcatTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGa                                                                             <  2:1199243/140‑1 (MQ=255)                                                         catTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGc                                                                           <  1:337987/140‑1 (MQ=255)                                                         catTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGc                                                                           <  2:1243348/140‑1 (MQ=255)                                                         catTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGc                                                                           <  1:157803/140‑1 (MQ=255)                                                           ttGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCg                                                                         <  1:1390616/140‑1 (MQ=255)                                                           ttGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCg                                                                         >  1:1120403/1‑140 (MQ=255)                                                            tgatgaCGCCGCTGAGGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGt                                                                        <  1:652075/140‑1 (MQ=255)                                                                gaCGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCa                                                                    >  1:960922/1‑140 (MQ=255)                                                                 aCGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGtct                                                                      <  1:674631/137‑1 (MQ=255)                                                                 aCGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGtct                                                                      >  2:674631/1‑137 (MQ=255)                                                                 aCGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCAc                                                                   <  2:1452785/140‑1 (MQ=255)                                                                 aCGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCAc                                                                   <  2:1120403/140‑1 (MQ=255)                                                                  cgccgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACg                                                                  >  1:255338/1‑140 (MQ=255)                                                                  cgccgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACg                                                                  >  2:39835/1‑140 (MQ=255)                                                                  cgccgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACg                                                                  <  2:498328/140‑1 (MQ=255)                                                                  cgccgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACg                                                                  >  2:481262/1‑140 (MQ=255)                                                                  cgccgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACg                                                                  >  2:1150270/1‑140 (MQ=255)                                                                   gccgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACga                                                                 >  1:1074861/1‑140 (MQ=255)                                                                     cgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACgaga                                                               >  2:1373566/1‑140 (MQ=255)                                                                     cgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACgaga                                                               >  2:393526/1‑140 (MQ=255)                                                                     cgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACg                                                                  <  1:681666/137‑1 (MQ=255)                                                                     cgcTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACg                                                                  >  2:681666/1‑137 (MQ=255)                                                                       cTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAat                                                             <  2:257655/140‑1 (MQ=255)                                                                       cTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAat                                                             <  1:694525/140‑1 (MQ=255)                                                                        tGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAata                                                            >  1:1281047/1‑140 (MQ=255)                                                                            gTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGc                                                        <  1:1150270/140‑1 (MQ=255)                                                                            gTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGc                                                        >  2:1559145/1‑140 (MQ=255)                                                                               gCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAat                                                             >  1:105653/1‑132 (MQ=255)                                                                               gCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAat                                                             <  2:105653/132‑1 (MQ=255)                                                                                   gTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTg                                                 <  1:571972/140‑1 (MQ=255)                                                                                   gTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTg                                                 <  1:393526/140‑1 (MQ=255)                                                                                    tCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGa                                                >  2:727680/1‑140 (MQ=255)                                                                                       gATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGc                                             <  2:1074861/140‑1 (MQ=255)                                                                                        aTGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCt                                            >  1:532916/1‑140 (MQ=255)                                                                                             aaaaTCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTAcc                                       <  1:1559145/140‑1 (MQ=255)                                                                                             aaaaTCGATGCCGCTGGTGAAAGAAAAACGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTAcc                                       <  2:1067610/140‑1 (MQ=255)                                                                                                 tCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGa                                   >  1:1015730/1‑140 (MQ=255)                                                                                                 tCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGa                                   <  2:1166175/140‑1 (MQ=255)                                                                                                      gCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAAc                              <  2:1591830/140‑1 (MQ=255)                                                                                                      gCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAAc                              <  1:1373566/140‑1 (MQ=255)                                                                                                           tGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCACTGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGtt                         <  2:1397440/140‑1 (MQ=255)                                                                                                           tGGTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGtt                         >  1:1348967/1‑140 (MQ=255)                                                                                                            ggTGAAAGAAAATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTg                        <  1:481262/140‑1 (MQ=255)                                                                                                                  agaaaATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTGAAGTgc                  >  2:1094341/1‑140 (MQ=255)                                                                                                                   gaaaATCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTGAAGTgct                 <  2:1015730/140‑1 (MQ=255)                                                                                                                        tCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTGAAGTgctggctg            >  2:1145053/1‑140 (MQ=255)                                                                                                                        tCGCCGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTGAAGTgctggctg            <  1:39835/140‑1 (MQ=255)                                                                                                                            cGTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTGAAGTGCTGGCTGCCGa        >  1:1178735/1‑140 (MQ=255)                                                                                                                             gTGATACAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTGAAGTGCTGGCTGCCGAc       <  2:443521/140‑1 (MQ=255)                                                                                                                                  aCAGGCAATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTGAAGTGCTGGCTGCCGACCTgcg  >  2:1057443/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      GACGAGTTTGTCGTGCATACGCTGGGTGAAAACGCGCGCCGCCATTACCGCATCATCATTGATGACGCCGCTGAAGTCGCTCGTCAGATGAAAAAATCGATGCCGCTGGTGAAAGAAAATCGCCGTGATACAGGCGATGCCTACAGCTTTAACTGGTCAATGCGCATTGCGCCAGATTTGCAAATGCCGTTTGAGCCGTCTCACGAGAATATGGCTAATCTGAAGCTTTACCCGGATCAACCTGTTGAAGTGCTGGCTGCCGACCTGCG  >  NC_000913/2927205‑2927473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑