Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,042,956 A→G F65L (TTC→CTC)  yqfA ← hemolysin‑III family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,042,9560AG98.9% 329.5 / NA 93F65L (TTC→CTC) yqfAhemolysin‑III family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (47/45);  minor base C (0/1);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGAATCCAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCCGTGACTGACGCT  >  NC_000913/3042820‑3043091
                                                                                                                                        |                                                                                                                                       
ggAGAATCCAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGcag                                                                                                                                      <  1:696489/140‑1 (MQ=255)
 gagaATCCAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGagcagc                                                                                                                                     <  1:151558/140‑1 (MQ=255)
      tCCAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGAt                                                                                                                                >  1:250113/1‑140 (MQ=255)
      tCCAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGAt                                                                                                                                >  2:152569/1‑140 (MQ=255)
        cAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCa                                                                                                                              >  2:450015/1‑140 (MQ=255)
          gCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATa                                                                                                                            >  2:1232205/1‑140 (MQ=255)
           ccccACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATAc                                                                                                                           >  1:1490679/1‑140 (MQ=255)
           ccccACCAACAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAgcagca                                                                                                                                    <  2:1302499/131‑1 (MQ=255)
           ccccACCAACAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAgcagca                                                                                                                                    >  1:1302499/1‑131 (MQ=255)
             ccaccaGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTg                                                                                                                         <  1:175145/140‑1 (MQ=255)
                ccaGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTgccg                                                                                                                      >  2:1559392/1‑140 (MQ=255)
                ccaGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTgccg                                                                                                                      <  1:1166822/140‑1 (MQ=255)
                     aaaaaCGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATa                                                                                                                 <  2:159843/140‑1 (MQ=255)
                     aaaaaCGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATa                                                                                                                 <  2:1241889/140‑1 (MQ=255)
                         aCGGCGTTGAGGTTCCGGCAATCAACAGGTAAATTGCGGAATGGTAAAATTTCTTCAGCCACATTTTGGCCCGTTGATGGGGAATGGCGTCATAGAGCGAGGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAgg                                                                                                             <  2:1187221/140‑1 (MQ=255)
                           ggCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCt                                                                                                           >  2:1081990/1‑140 (MQ=255)
                             cGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGt                                                                                                         >  1:1358109/1‑140 (MQ=255)
                              gtgtAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTCCCGCCATAGAGGCTGTa                                                                                                        >  1:125716/1‑140 (MQ=255)
                               tgtAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAg                                                                                                       <  1:883050/140‑1 (MQ=255)
                               tgtAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAg                                                                                                       <  1:102776/140‑1 (MQ=255)
                               tgtAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAg                                                                                                       <  1:140747/140‑1 (MQ=255)
                               tgtAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAg                                                                                                       <  2:915476/140‑1 (MQ=255)
                               tgtAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGACCAGCAGGATCATACTGCCGCCATAGAGGCTGTAg                                                                                                       <  2:1422866/140‑1 (MQ=255)
                                 tAGGTCCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCt                                                                                                     <  2:1159198/140‑1 (MQ=255)
                                    gTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGt                                                                                                  <  2:250113/140‑1 (MQ=255)
                                    gTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGt                                                                                                  <  1:430323/140‑1 (MQ=255)
                                       ccGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTAt                                                                                               <  2:787296/140‑1 (MQ=255)
                                       ccGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTAt                                                                                               >  2:723029/1‑140 (MQ=255)
                                         ggCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCg                                                                                             >  1:1583646/1‑140 (MQ=255)
                                         ggCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCg                                                                                             >  1:1388703/1‑140 (MQ=255)
                                           cAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCt                                                                                           <  1:723029/140‑1 (MQ=255)
                                               cAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTgg                                                                                       <  1:960330/140‑1 (MQ=255)
                                               cAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTgg                                                                                       <  1:220107/140‑1 (MQ=255)
                                                  cAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcgc                                                                                    <  1:528325/140‑1 (MQ=255)
                                                  cAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcgc                                                                                    <  1:247325/140‑1 (MQ=255)
                                                  cAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTCCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcgc                                                                                    <  2:125716/140‑1 (MQ=255)
                                                   aGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCt                                                                                   <  1:152569/140‑1 (MQ=255)
                                                     gTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTgg                                                                                 <  2:1583646/140‑1 (MQ=255)
                                                         aTAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCAtt                                                                             <  1:993469/140‑1 (MQ=255)
                                                            gcgcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcgc                                                                                    <  2:751802/130‑1 (MQ=255)
                                                            gcgcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGgcgc                                                                                    >  1:751802/1‑130 (MQ=255)
                                                             cgcAATTGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGa                                                                         >  1:280851/1‑140 (MQ=255)
                                                             cgcAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGa                                                                         >  1:753598/1‑140 (MQ=255)
                                                               cAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGTCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATc                                                                       >  1:851733/1‑140 (MQ=255)
                                                               cAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATc                                                                       >  1:126266/1‑140 (MQ=255)
                                                               cAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATc                                                                       >  2:1244789/1‑140 (MQ=255)
                                                               cAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATc                                                                       >  2:303982/1‑140 (MQ=255)
                                                                 aTGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCa                                                                     <  1:1234106/140‑1 (MQ=255)
                                                                 aTGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCa                                                                     <  2:1187325/140‑1 (MQ=255)
                                                                  tGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc                                                                    >  1:1208626/1‑140 (MQ=255)
                                                                  tGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc                                                                    >  2:575681/1‑140 (MQ=255)
                                                                  tGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc                                                                    >  2:769013/1‑140 (MQ=255)
                                                                  tGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc                                                                    >  1:670319/1‑140 (MQ=255)
                                                                  tGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCAc                                                                    >  1:589769/1‑140 (MQ=255)
                                                                    gTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTaa                                                                           <  1:846147/131‑1 (MQ=255)
                                                                    gTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTaa                                                                           >  2:846147/1‑131 (MQ=255)
                                                                    gTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCg                                                                  >  2:847173/1‑140 (MQ=255)
                                                                    gTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCg                                                                  >  1:370668/1‑140 (MQ=255)
                                                                    gTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCg                                                                  >  2:857298/1‑140 (MQ=255)
                                                                    gTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCg                                                                  <  1:598598/140‑1 (MQ=255)
                                                                       aaaTTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCt                                                               >  1:1288725/1‑140 (MQ=255)
                                                                       aaaTTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCACCt                                                               >  2:1039858/1‑140 (MQ=255)
                                                                        aaTTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGTTCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTg                                                              <  2:589769/140‑1 (MQ=255)
                                                                           ttcttcAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCACCTGAAc                                                           <  1:1039858/140‑1 (MQ=255)
                                                                             cttcAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCa                                                         <  2:753598/140‑1 (MQ=255)
                                                                             cttcAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCa                                                         <  2:1358109/140‑1 (MQ=255)
                                                                              ttcAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAg                                                        >  1:298358/1‑140 (MQ=255)
                                                                                 aGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAg                                                     <  2:657580/140‑1 (MQ=255)
                                                                                 aGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAg                                                     <  1:1496435/140‑1 (MQ=255)
                                                                                  gCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGc                                                    <  1:847173/140‑1 (MQ=255)
                                                                                       aTTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGcc                                                                >  2:1005300/1‑123 (MQ=255)
                                                                                       aTTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGcc                                                                <  1:1005300/123‑1 (MQ=255)
                                                                                          tttGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAg                                            <  2:370668/140‑1 (MQ=255)
                                                                                             gCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGccc                                         >  1:403410/1‑140 (MQ=255)
                                                                                               ccGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCaa                                       <  2:1288725/140‑1 (MQ=255)
                                                                                                            aTGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGATGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAacac                          >  2:991720/1‑140 (MQ=255)
                                                                                                             tGGCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACAcc                         >  1:681547/1‑140 (MQ=255)
                                                                                                               gCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCaa                       >  2:1057952/1‑140 (MQ=255)
                                                                                                               gCGTGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCaa                       >  1:1274421/1‑140 (MQ=255)
                                                                                                                  tGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAacac                          >  2:1193894/1‑134 (MQ=255)
                                                                                                                  tGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAacac                          <  1:1117531/134‑1 (MQ=255)
                                                                                                                  tGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAacac                          <  1:1193894/134‑1 (MQ=255)
                                                                                                                  tGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAAccc                    >  1:74755/1‑140 (MQ=255)
                                                                                                                  tGATAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTAGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAacac                          >  2:1117531/1‑134 (MQ=255)
                                                                                                                    aTAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAacac                          >  1:443865/1‑132 (MQ=255)
                                                                                                                    aTAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAacac                          <  2:443865/132‑1 (MQ=255)
                                                                                                                    aTAGAGCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCaa                  >  1:771207/1‑140 (MQ=255)
                                                                                                                        agCGTCGAAGCGAGGAGCAGCAGGATCATACTGTCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGcc              <  2:851733/140‑1 (MQ=255)
                                                                                                                        agCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATGGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGcc              >  1:486236/1‑140 (MQ=255)
                                                                                                                        agCGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGcc              <  2:403410/140‑1 (MQ=255)
                                                                                                                          cGTCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCCGt            >  1:841922/1‑140 (MQ=255)
                                                                                                                            tCGAAGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCCGtga          >  1:1205360/1‑140 (MQ=255)
                                                                                                                               aaGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCCGtgtctg       >  1:1576351/1‑140 (MQ=255)
                                                                                                                                aGCGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCCGtgactga      <  1:207146/140‑1 (MQ=255)
                                                                                                                                  cGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCCGTGACTGACg    <  2:126266/140‑1 (MQ=255)
                                                                                                                                  cGAGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGGTATCGCCGTTGCGCTGGCATTAAGATCCACCGCCTCAACCAGTAACTACACCAGCCCAAGGAAACGAAACCGCAAGCCAATGCCGAGACCGACg    >  2:1148821/1‑140 (MQ=255)
                                                                                                                                    aGGAGCAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCCGTGACTGACGCt  <  2:670319/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GGAGAATCCAGCCCCACCAGCAAAAACGGCGTGTAGGTTCCGGCAATCAACAGGTAAATAGCGCAATGGTCAAATTTCTTCAGCCACATTTTTGCCCGTTGATGAGGAATGGCGTGATAGAGCGTCGAAGCGAGGAACAGCAGGATCATACTGCCGCCATAGAGGCTGTAGCTGGTTATCGCTGTGGCGCTGGCATTAAGATCCACCGCCTGAACCAGTAGCAACACCAGCCCAACGATACCAAACACCAACCCAATGCCGTGACTGACGCT  >  NC_000913/3042820‑3043091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑