| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,143,408 | T→C | T253A (ACC→GCC) | hybB ← | hydrogenase 2 membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,143,408 | 0 | T | C | 97.7% | 145.6 / ‑5.8 | 44 | T253A (ACC→GCC) | hybB | hydrogenase 2 membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (22/21); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGCCCATGATGAACGCCGTCAGCAGCGAGAACAGCGGCAA > NC_000913/3143270‑3143540 | gCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCT‑‑CCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGtt > 2:1014723/1‑140 (MQ=255) gggAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGtt > 1:178916/1‑140 (MQ=255) gAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTgg < 1:1594551/140‑1 (MQ=255) aaGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGt < 2:906673/140‑1 (MQ=255) aaGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGt < 2:2432/140‑1 (MQ=255) aaGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGt < 1:1502799/140‑1 (MQ=255) aaGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCT‑‑CCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCa < 1:1014723/140‑1 (MQ=255) gagCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCa < 1:750185/140‑1 (MQ=255) gagCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCa < 1:1225731/140‑1 (MQ=255) agGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTaacaaa < 1:689102/140‑1 (MQ=255) ggACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTa < 2:867409/134‑1 (MQ=255) ggACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTa > 1:867409/1‑134 (MQ=255) gACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTaacaaaca > 2:608646/1‑140 (MQ=255) gACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTaacaaaca < 2:1463785/140‑1 (MQ=255) cTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGa < 2:662735/140‑1 (MQ=255) cTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGa < 1:1246095/140‑1 (MQ=255) cTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGa < 1:1163608/140‑1 (MQ=255) cTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGa < 1:318774/140‑1 (MQ=255) cAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGActc > 2:1006685/1‑140 (MQ=255) aTCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCtt < 1:1006685/140‑1 (MQ=255) aaCATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATc < 2:537376/140‑1 (MQ=255) aaCATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATc < 1:608646/140‑1 (MQ=255) cATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAACACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCg < 2:998530/140‑1 (MQ=255) cACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGAc < 1:823735/140‑1 (MQ=255) cACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGAc < 1:113774/140‑1 (MQ=255) aCCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGAc > 1:565711/1‑140 (MQ=255) cGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGAccc > 1:1190156/1‑140 (MQ=255) cGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTACCACGCAGAccc > 1:109178/1‑140 (MQ=255) gCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCTAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGc > 2:841374/1‑140 (MQ=255) gCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAATCAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCt > 1:678111/1‑140 (MQ=255) cTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCAGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCtt > 1:237062/1‑140 (MQ=255) tCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCaaa > 1:1510487/1‑140 (MQ=255) cgcgATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAg > 1:291380/1‑140 (MQ=255) accTCGCAAAAGCGCAGCACGAAAAAAACCGCCAGCAACACACTGAGGGGTTTGGTCAGCTAAACAAACAGCCTCTTTCCATCCGGACCGTTCCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCga < 2:1434578/138‑1 (MQ=255) aGCTCCCTAAGGCGCAGCACGAAAAAAACCGCCACCAACACACGAATGGCGTGGGTTAGTTTAAAAAACAGCCTCTTTTCATCAGGACCTTGCCCACGCAGTCCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCga < 2:843300/140‑1 (MQ=255) cTCGCAAAAGCGCAGCACGAAAAAAACCGCCAGCAACACACTGATGGGGTTGGTCAGCTTAAAAAACAGACTCTTTCCATCCGGCCCGTTGCCACGCAGCCCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCgaga < 2:625266/140‑1 (MQ=255) gCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGc > 1:1523532/1‑140 (MQ=255) ccAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGcc > 1:1434578/1‑140 (MQ=255) ccAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGcc > 1:247573/1‑140 (MQ=255) ccAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGcc > 1:990082/1‑140 (MQ=255) ccAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGcc > 1:434028/1‑140 (MQ=255) cAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGccc > 1:242189/1‑140 (MQ=255) cAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCCAACCTTCAAAGATGACAAACGAGAAGccc > 2:1180397/1‑140 (MQ=255) agcaCGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGCCCATGATGaa > 1:562065/1‑140 (MQ=255) agcaCGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACACGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGCCCATGATGaa > 2:468109/1‑140 (MQ=255) gcaCGATGAAAATCGCCAGCAACACACTGATGGCGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGCCCATGATGAAc < 1:138624/140‑1 (MQ=255) tGAAAATCGCCAGCAACACACTGATGGTGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGCCCATGATGAACGCCGTc > 2:1384803/1‑140 (MQ=255) gCAACACACTGAGGGGGTTGTTCAGCTTAACAAACACACTCTTTTCTTCCGAACGGTTGCCAGGCGGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGCCCATGATGAACGCCGTCAGCAGCGAGAAc < 2:1488426/140‑1 (MQ=255) ccacTGATGGCGTGGGTCAGCTTAACAAACAGCCTCTTTTCATCCGGACCGTTGCCACGCAGCCCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGCCCATGATGAACGCCGTCAGCAGCGAGAACAGCgg < 2:562065/139‑1 (MQ=255) cTGATGGGGTTGGTCAGCTAAAAAAACAGACTTTTTTCACCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGCCCATGATGAACGCCGTCAGCAGCGAGAACAGCGGCaa < 2:434028/140‑1 (MQ=255) | GCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGTCAGCTTAACAAACAGACTCTTTTCATCCGGACCGTTGCCACGCAGACCCGCCTGCACCAGCGAACCTTCAAAGATGACAATCGAGAAGCCCATGATGAACGCCGTCAGCAGCGAGAACAGCGGCAA > NC_000913/3143270‑3143540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |