BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,270,859	T→C	V304V (GTA→GTG)	garK ←	glycerate 2‑kinase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,859	0	T	C	100.0%	415.5 / NA	115	V304V (GTA→GTG)	garK	glycerate 2‑kinase 1
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (58/57); total (58/57)

Only 100 of 118 total aligned reads displayed.

CTATGCTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCACCAAGAAAC  >  NC_000913/3270726‑3270994

cTATGCTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTc                                                                                                                                   >  1:386346/1‑140 (MQ=255)

  aTGCTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTccc                                                                                                                                 <  2:825913/140‑1 (MQ=255)

    gCTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGt                                                                                                                               >  1:418099/1‑140 (MQ=255)

     cTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCAcc                                                                                                                                      >  2:962839/1‑132 (MQ=255)

     cTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCAcc                                                                                                                                      <  1:962839/132‑1 (MQ=255)

       ggTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGaa                                                                                                                            <  1:216120/140‑1 (MQ=255)

       ggTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGaa                                                                                                                            <  1:128953/140‑1 (MQ=255)

       ggTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGaa                                                                                                                            >  2:62837/1‑140 (MQ=255)

        gTCAATACGCTGAAGACCGCATCAATGCCATGCTGTTGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAAt                                                                                                                           >  2:1363710/1‑140 (MQ=255)

        gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTcc                                                                                                                                  <  2:1248556/133‑1 (MQ=255)

        gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTcc                                                                                                                                  >  1:1248556/1‑133 (MQ=255)

        gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTc                                                                                                                                   <  2:381260/132‑1 (MQ=255)

        gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTc                                                                                                                                   >  1:381260/1‑132 (MQ=255)

        gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAAt                                                                                                                           >  1:897336/1‑140 (MQ=255)

        gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAAt                                                                                                                           >  1:1182100/1‑140 (MQ=255)

          cAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATAc                                                                                                                         <  2:1309352/140‑1 (MQ=255)

          cAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATAc                                                                                                                         <  2:1131823/140‑1 (MQ=255)

           aaTACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATAct                                                                                                                        <  1:119605/140‑1 (MQ=255)

            aTACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATActc                                                                                                                       <  1:62837/140‑1 (MQ=255)

             tACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTcc                                                                                                                                  <  2:546068/128‑1 (MQ=255)

             tACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTcc                                                                                                                                  >  1:546068/1‑128 (MQ=255)

                 cTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCt                                                                                                                  <  2:22040/140‑1 (MQ=255)

                 cTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCt                                                                                                                  <  1:183385/140‑1 (MQ=255)

                  tGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACTTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTg                                                                                                                 >  2:1481816/1‑140 (MQ=255)

                  tGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTg                                                                                                                 >  2:789313/1‑140 (MQ=255)

                       aCCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAAt                                                                                                            <  1:948072/140‑1 (MQ=255)

                       aCCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAAt                                                                                                            >  1:907900/1‑140 (MQ=255)

                        ccGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATa                                                                                                           <  2:418099/140‑1 (MQ=255)

                         cGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCt                                                                                                                  >  1:624148/1‑132 (MQ=255)

                         cGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCt                                                                                                                  <  2:624148/132‑1 (MQ=255)

                         cGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATAc                                                                                                          >  1:270889/1‑140 (MQ=255)

                         cGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATAc                                                                                                          >  2:332976/1‑140 (MQ=255)

                         cGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATAc                                                                                                          >  2:67407/1‑140 (MQ=255)

                          gCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACg                                                                                                         <  1:242773/140‑1 (MQ=255)

                           cATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGc                                                                                                        >  1:852339/1‑140 (MQ=255)

                            aTCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGcc                                                                                                       >  1:1065242/1‑140 (MQ=255)

                            aTCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGcc                                                                                                       <  2:880152/140‑1 (MQ=255)

                             tCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGccc                                                                                                      <  1:364324/140‑1 (MQ=255)

                                aTGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTc                                                                                                   >  1:957875/1‑140 (MQ=255)

                                  gCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCAc                                                                                                 <  1:789313/140‑1 (MQ=255)

                                  gCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCAc                                                                                                 <  2:386346/140‑1 (MQ=255)

                                    cATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCg                                                                                               <  1:67407/140‑1 (MQ=255)

                                      tGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGt                                                                                             >  2:140655/1‑140 (MQ=255)

                                           aTGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCa                                                                                        >  2:46180/1‑140 (MQ=255)

                                           aTGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCa                                                                                        >  1:1376761/1‑140 (MQ=255)

                                            tGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCAc                                                                                       <  2:1182100/140‑1 (MQ=255)

                                             gTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCAcc                                                                                      >  2:1008367/1‑140 (MQ=255)

                                                cAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGc                                                                                   >  1:1061504/1‑140 (MQ=255)

                                                  aCGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCAc                                                                                                 <  2:1308400/124‑1 (MQ=255)

                                                  aCGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGt                                                                                 >  1:375318/1‑140 (MQ=255)

                                                  aCGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGt                                                                                 >  1:983477/1‑140 (MQ=255)

                                                  aCGCCAACATCATCGGTCAGGCTACCCGCAATACCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCAc                                                                                                 >  1:1308400/1‑124 (MQ=255)

                                                   cGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGT‑CTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCg                                                                                  >  1:1520207/1‑137 (MQ=255)

                                                   cGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGT‑CTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCg                                                                                  <  2:1520207/137‑1 (MQ=255)

                                                     ccAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACTGGTGATCACCAGCGTACa                                                                              <  2:453998/140‑1 (MQ=255)

                                                     ccAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACa                                                                              >  1:1048016/1‑140 (MQ=255)

                                                     ccAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACa                                                                              >  1:151223/1‑140 (MQ=255)

                                                      cAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACaa                                                                             >  2:1031584/1‑140 (MQ=255)

                                                      cAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACaa                                                                             <  1:140655/140‑1 (MQ=255)

                                                       aaCATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAAt                                                                            >  1:1483038/1‑140 (MQ=255)

                                                        aCATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATc                                                                           >  2:247557/1‑140 (MQ=255)

                                                         catcatCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCg                                                                          <  2:907900/140‑1 (MQ=255)

                                                                ggTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAatat                                                                   <  2:1065242/140‑1 (MQ=255)

                                                                 gTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATg                                                                  <  2:1048016/140‑1 (MQ=255)

                                                                    aGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTtc                                                               >  2:1545156/1‑140 (MQ=255)

                                                                      gCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTtcct                                                             <  2:852339/140‑1 (MQ=255)

                                                                          cccGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAg                                                         >  1:995735/1‑140 (MQ=255)

                                                                                aTGTCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAg                                                   >  2:942100/1‑140 (MQ=255)

                                                                                  gCCAATCATCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAgcg                                                 <  1:524239/140‑1 (MQ=255)

                                                                                  gCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAgcg                                                 <  1:1342953/140‑1 (MQ=255)

                                                                                  gCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAgcg                                                 <  1:1008367/140‑1 (MQ=255)

                                                                                    cAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGcc                                               >  2:1288261/1‑140 (MQ=255)

                                                                                       tCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTa                                            <  1:380779/140‑1 (MQ=255)

                                                                                          ccGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTg                                         <  1:719701/140‑1 (MQ=255)

                                                                                           cGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGa                                        >  1:1412665/1‑140 (MQ=255)

                                                                                            ggTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGAc                                       <  1:1481816/140‑1 (MQ=255)

                                                                                             gTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACg                                      <  1:1470219/140‑1 (MQ=255)

                                                                                                    gTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCaa                               <  2:1483038/140‑1 (MQ=255)

                                                                                                    gTACTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCaa                               <  2:1061504/140‑1 (MQ=255)

                                                                                                      aCTTCTTCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATa                             <  2:375318/140‑1 (MQ=255)

                                                                                                       cttcttCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATAc                            <  1:1031584/140‑1 (MQ=255)

                                                                                                       cttcttCGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATAc                            >  2:1466693/1‑140 (MQ=255)

                                                                                                         tcttcGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCa                          <  2:151223/140‑1 (MQ=255)

                                                                                                         tcttcGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCa                          <  1:1363710/140‑1 (MQ=255)

                                                                                                          cttcGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCAc                         >  2:1549268/1‑140 (MQ=255)

                                                                                                          cttcGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCAc                         >  2:1355843/1‑140 (MQ=255)

                                                                                                            tcGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACtt                       >  2:733738/1‑140 (MQ=255)

                                                                                                             cGCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACttt                      >  1:1494136/1‑140 (MQ=255)

                                                                                                              gCCACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACtttt                     >  2:1068120/1‑140 (MQ=255)

                                                                                                               ccACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTc                    >  1:1125230/1‑140 (MQ=255)

                                                                                                               ccACGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTc                    >  1:74607/1‑140 (MQ=255)

                                                                                                                  cGTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGt                 >  2:585923/1‑140 (MQ=255)

                                                                                                                   gTTTGCGACACCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGtt                <  2:957875/140‑1 (MQ=255)

                                                                                                                    tttGCGACACCAATCGGCACCTTCCCGTGACTACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTc               <  2:983477/140‑1 (MQ=255)

                                                                                                                          acacCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCTTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCAcc         <  2:1411626/140‑1 (MQ=255)

                                                                                                                          acacCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCAcc         >  2:347127/1‑140 (MQ=255)

                                                                                                                            acCAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCACCaa       <  2:270889/140‑1 (MQ=255)

                                                                                                                              cAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCACCAAGa     >  2:886428/1‑140 (MQ=255)

                                                                                                                              cAATCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCACCAAGa     >  1:108044/1‑140 (MQ=255)

                                                                                                                                 tCGGCACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATGCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCACCAAGAAAc  <  1:263297/140‑1 (MQ=255)

CTATGCTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCACCAAGAAAC  >  NC_000913/3270726‑3270994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`