Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,386,207	G→T	intergenic (‑78/+14)	yhcO ← / ← aaeB	putative barnase inhibitor/aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,386,207	0	G	T	100.0%	300.7 / NA	87	intergenic (‑78/+14)	yhcO/aaeB	putative barnase inhibitor/aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (46/41);  total (46/41)

TCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGT  >  NC_000913/3386071‑3386338                                                                                                                                         |                                                                                                                                    tCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGt                                                                                                                                  <  2:1503356/140‑1 (MQ=255) tCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGt                                                                                                                                  <  2:205322/140‑1 (MQ=255) tCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGt                                                                                                                                  <  2:227714/140‑1 (MQ=255) tCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGt                                                                                                                                  <  2:504020/140‑1 (MQ=255)    cGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCgg                                                                                                                               >  1:486628/1‑140 (MQ=255)    cGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCgg                                                                                                                               >  1:565717/1‑140 (MQ=255)     gATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGt                                                                                                                              <  1:212689/140‑1 (MQ=255)        aaaaaTCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGtttt                                                                                                                           <  1:817266/140‑1 (MQ=255)           aaTCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGAc                                                                                                                        <  1:103411/140‑1 (MQ=255)               ctcctGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTaa                                                                                                                    >  2:1332429/1‑140 (MQ=255)                   tGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTAt                                                                                                                <  2:486628/140‑1 (MQ=255)                   tGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTAt                                                                                                                <  2:278513/140‑1 (MQ=255)                      ctctCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCgg                                                                                                             <  2:912226/140‑1 (MQ=255)                            aTCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACg                                                                                                       <  1:1032775/140‑1 (MQ=255)                            aTCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACg                                                                                                       <  1:1108511/140‑1 (MQ=255)                            aTCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACg                                                                                                       <  2:1131148/140‑1 (MQ=255)                               tcatcaAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCAt                                                                                                    >  1:1206863/1‑140 (MQ=255)                                 atcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCgg                                                                                                             <  2:1516491/129‑1 (MQ=255)                                 atcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCgg                                                                                                             >  1:1516491/1‑129 (MQ=255)                                 atcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCgg                                                                                                             <  2:424891/129‑1 (MQ=255)                                 atcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCgg                                                                                                             >  1:424891/1‑129 (MQ=255)                                 atcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTc                                                                                                           >  2:648521/1‑131 (MQ=255)                                 atcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTc                                                                                                           <  1:648521/131‑1 (MQ=255)                                 atcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGt                                                                                                  >  1:578344/1‑140 (MQ=255)                                 atcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGt                                                                                                  >  1:1195360/1‑140 (MQ=255)                                  tcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGtt                                                                                                 <  1:571682/140‑1 (MQ=255)                                    aaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGa                                                                                               <  2:1041380/140‑1 (MQ=255)                                    aaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGa                                                                                               <  1:960563/140‑1 (MQ=255)                                    aaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGa                                                                                               <  2:992687/140‑1 (MQ=255)                                    aaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGa                                                                                               <  1:1332429/140‑1 (MQ=255)                                     aaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGAt                                                                                              >  1:200018/1‑140 (MQ=255)                                     aaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGAt                                                                                              <  1:698329/140‑1 (MQ=255)                                       atcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATAc                                                                                            >  2:769973/1‑140 (MQ=255)                                        tcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACt                                                                                           <  2:1308040/140‑1 (MQ=255)                                                ataAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGa                                                                                               <  2:482993/128‑1 (MQ=255)                                                ataAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGa                                                                                               >  1:482993/1‑128 (MQ=255)                                                 taAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCa                                                                                  <  2:565717/140‑1 (MQ=255)                                                        tCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTTTGGAGCATCCCCGc                                                                           >  1:128082/1‑140 (MQ=255)                                                        tCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGc                                                                           >  2:300189/1‑140 (MQ=255)                                                        tCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGc                                                                           >  2:1551273/1‑140 (MQ=255)                                                         cATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGcc                                                                          >  2:74393/1‑140 (MQ=255)                                                          aTCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCa                                                                         >  2:525509/1‑140 (MQ=255)                                                             tGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGcc                                                                      >  2:596178/1‑140 (MQ=255)                                                               ttGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGa                                                                    >  2:808017/1‑140 (MQ=255)                                                               ttGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGa                                                                    >  1:1080177/1‑140 (MQ=255)                                                                tGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGAt                                                                   >  2:115314/1‑140 (MQ=255)                                                                    cccGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTAc                                                               <  2:633588/139‑1 (MQ=255)                                                                    aCCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTAc                                                               >  2:689933/1‑140 (MQ=255)                                                                    aCCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTAc                                                               <  1:808017/140‑1 (MQ=255)                                                                      cGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCg                                                             >  2:46834/1‑140 (MQ=255)                                                                         cACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTAcc                                                              <  2:961435/136‑1 (MQ=255)                                                                         cACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTAcc                                                              >  1:961435/1‑136 (MQ=255)                                                                         cACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGtt                                                          >  2:343337/1‑140 (MQ=255)                                                                           cGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTcc                                                        <  1:74393/140‑1 (MQ=255)                                                                                cgcgGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCAc                                                   <  2:1195360/140‑1 (MQ=255)                                                                                cgcgGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCAc                                                   >  2:686205/1‑140 (MQ=255)                                                                                cgcgGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCAc                                                   <  1:1604412/140‑1 (MQ=255)                                                                                  cggcgAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCt                                                 >  2:203447/1‑140 (MQ=255)                                                                                  cggcgAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCt                                                 >  2:864875/1‑140 (MQ=255)                                                                                     cgAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCg                                              >  2:638330/1‑140 (MQ=255)                                                                                           cTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGgcg                                        <  1:821460/140‑1 (MQ=255)                                                                                           cTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGgcg                                        <  1:638330/140‑1 (MQ=255)                                                                                           cTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGgcg                                        <  2:1080177/140‑1 (MQ=255)                                                                                                   cATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAg                                >  1:1541313/1‑140 (MQ=255)                                                                                                             tataGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCt                      <  1:46834/140‑1 (MQ=255)                                                                                                                aGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAg                                >  2:1080576/1‑127 (MQ=255)                                                                                                                aGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAg                                <  1:1080576/127‑1 (MQ=255)                                                                                                                aGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCtttt                   >  1:1326413/1‑140 (MQ=255)                                                                                                                aGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCtttt                   >  1:1290172/1‑140 (MQ=255)                                                                                                                aGCCATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCtttt                   >  2:586447/1‑140 (MQ=255)                                                                                                                  ccATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTcc                 >  1:1019978/1‑140 (MQ=255)                                                                                                                  ccATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTcc                 >  1:265277/1‑140 (MQ=255)                                                                                                                  ccATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTcc                 >  2:1233657/1‑140 (MQ=255)                                                                                                                  ccATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTcc                 <  1:1568651/140‑1 (MQ=255)                                                                                                                  ccATGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGATTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTcc                 >  1:1551470/1‑140 (MQ=255)                                                                                                                     tGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTc                  >  1:1595498/1‑136 (MQ=255)                                                                                                                     tGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTc                  <  2:1595498/136‑1 (MQ=255)                                                                                                                     tGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTgg              >  1:305138/1‑140 (MQ=255)                                                                                                                     tGAAAAAACCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTgg              >  1:368768/1‑140 (MQ=255)                                                                                                                       aaaaaaCCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTa            <  1:203447/140‑1 (MQ=255)                                                                                                                       aaaaaaCCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTa            >  2:783138/1‑140 (MQ=255)                                                                                                                       aaaaaaCCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTa            >  2:863458/1‑140 (MQ=255)                                                                                                                       aaaaaaCCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTa            >  1:578565/1‑140 (MQ=255)                                                                                                                       aaaaaaCCGACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTa            >  1:1254153/1‑140 (MQ=255)                                                                                                                               gACGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCa    <  1:586447/140‑1 (MQ=255)                                                                                                                                 cGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGt  <  2:578344/140‑1 (MQ=255)                                                                                                                                 cGCTTTTGTCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGt  >  2:194810/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    TCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGT  >  NC_000913/3386071‑3386338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑