Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,462,381	T→C	V13A (GTC→GCC)	gspJ →	Type II secretion system protein GspJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,462,381	0	T	C	100.0%	290.7 / NA	80	V13A (GTC→GCC)	gspJ	Type II secretion system protein GspJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/31);  total (49/31)

AACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTT  >  NC_000913/3462254‑3462517                                                                                                                                |                                                                                                                                         aaCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCt                                                                                                                              <  1:1014842/140‑1 (MQ=255)   cGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTgg                                                                                                                            >  1:1429311/1‑140 (MQ=255)     cACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGTCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCg                                                                                                                          >  1:690756/1‑140 (MQ=255)     cACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATggcg                                                                                                                                   <  1:515613/131‑1 (MQ=255)     cACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATggcg                                                                                                                                   >  2:515613/1‑131 (MQ=255)     cACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCg                                                                                                                          >  2:1413419/1‑140 (MQ=255)       ccATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGAt                                                                                                                        <  2:269460/140‑1 (MQ=255)            aCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTtct                                                                                                                   <  2:399824/140‑1 (MQ=255)              ggTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCa                                                                                                                 <  2:188764/140‑1 (MQ=255)              ggTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCa                                                                                                                 <  1:1554482/140‑1 (MQ=255)                tGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAAt                                                                                                               <  2:1009501/140‑1 (MQ=255)                tGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAAt                                                                                                               >  1:314626/1‑140 (MQ=255)                  aCATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTtct                                                                                                                   >  2:1216725/1‑134 (MQ=255)                  aCATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTtct                                                                                                                   <  1:1216725/134‑1 (MQ=255)                           aGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGc                                                                                                    >  2:357932/1‑140 (MQ=255)                           aGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGc                                                                                                    <  2:314626/140‑1 (MQ=255)                           aGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGc                                                                                                    <  2:369665/140‑1 (MQ=255)                              ggTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTgg                                                                                                 >  1:97248/1‑140 (MQ=255)                                tCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCg                                                                                               >  2:843829/1‑140 (MQ=255)                                        ccTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAt                                                                                       >  1:1539062/1‑140 (MQ=255)                                           cGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAtttt                                                                                    >  1:595423/1‑140 (MQ=255)                                                aCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGc                                                                               >  1:988686/1‑140 (MQ=255)                                                  gcgTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCaa                                                                             >  2:298498/1‑140 (MQ=255)                                                       aTCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTc                                                                        >  2:93220/1‑140 (MQ=255)                                                       aTCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTc                                                                        <  1:473084/140‑1 (MQ=255)                                                       aTCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTc                                                                        <  2:1556059/140‑1 (MQ=255)                                                       aTCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTCTCCAAATGCGTAGAGTGCTGGCGTTTATGATTTTATCGCACGCCTc                                                                        <  2:1429311/140‑1 (MQ=255)                                                        tCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGTTTTTTTCGCAAGCTTcc                                                                       >  1:1595033/1‑140 (MQ=255)                                                            aGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGt                                                                   >  2:1129092/1‑140 (MQ=255)                                                            aGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGt                                                                   >  2:475438/1‑140 (MQ=255)                                                                tCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCa                                                               >  1:484717/1‑140 (MQ=255)                                                                tCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCa                                                               >  2:1323158/1‑140 (MQ=255)                                                                  gATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATc                                                             >  1:170427/1‑140 (MQ=255)                                                                  gATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATc                                                             >  2:86769/1‑140 (MQ=255)                                                                  gATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATc                                                             >  1:1167504/1‑140 (MQ=255)                                                                  gATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATc                                                             >  1:330150/1‑140 (MQ=255)                                                                      ataaATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACg                                                         >  2:211584/1‑140 (MQ=255)                                                                       taaATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGc                                                        <  1:298498/140‑1 (MQ=255)                                                                         aaTCCGGGCAGACACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTc                                                      >  2:783041/1‑140 (MQ=255)                                                                           tCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTcac                                                    <  1:1129092/140‑1 (MQ=255)                                                                              gggCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACaga                                                 >  1:957899/1‑140 (MQ=255)                                                                               ggcaggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaa                                                >  2:629262/1‑140 (MQ=255)                                                                               ggcaggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaa                                                >  1:525466/1‑140 (MQ=255)                                                                               ggcaggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaa                                                >  2:846212/1‑140 (MQ=255)                                                                               ggcaggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaa                                                >  1:1256436/1‑140 (MQ=255)                                                                               ggcagacaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaa                                                <  1:783041/140‑1 (MQ=255)                                                                                gcaggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACagaaa                                               >  2:1228669/1‑140 (MQ=255)                                                                                   ggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTca                                                     <  1:305577/131‑1 (MQ=255)                                                                                   ggcaCAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTca                                                     >  2:305577/1‑131 (MQ=255)                                                                                        aaGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCca                                       <  1:846212/140‑1 (MQ=255)                                                                                         aGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCcag                                      >  2:320713/1‑140 (MQ=255)                                                                                         aGATGATTAATCGCCACCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCcag                                      <  2:1437897/140‑1 (MQ=255)                                                                                          gatgatTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCcagc                                     >  1:95650/1‑140 (MQ=255)                                                                                              atTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                 >  2:616144/1‑140 (MQ=255)                                                                                              atTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                 >  1:448473/1‑140 (MQ=255)                                                                                              atTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                 <  2:151710/140‑1 (MQ=255)                                                                                              atTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                 <  1:86769/140‑1 (MQ=255)                                                                                              atTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                 >  1:1399851/1‑140 (MQ=255)                                                                                              atTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                 >  1:999709/1‑140 (MQ=255)                                                                                              atTAATCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                 <  2:690756/140‑1 (MQ=255)                                                                                               ttAATCGCCAGCAGGGCTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTc                                >  2:828738/1‑140 (MQ=255)                                                                                                tAATCGCCAGTAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCa                               <  2:988686/140‑1 (MQ=255)                                                                                                 aaTCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGTTTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCaa                              <  2:1595033/140‑1 (MQ=255)                                                                                                  aTCGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAAt                             >  2:473431/1‑140 (MQ=255)                                                                                                    cGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                 >  1:119509/1‑134 (MQ=255)                                                                                                    cGCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGtt                                 <  2:119509/134‑1 (MQ=255)                                                                                                     gCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCcagcag                                   <  1:189587/131‑1 (MQ=255)                                                                                                     gCCAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCcagcag                                   >  2:189587/1‑131 (MQ=255)                                                                                                      ccAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGc                         >  2:881069/1‑140 (MQ=255)                                                                                                      ccAGCAGGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTCAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGc                         >  1:272021/1‑140 (MQ=255)                                                                                                         gcagGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTAc                      <  1:357932/140‑1 (MQ=255)                                                                                                          cagGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCca                                       <  1:348933/122‑1 (MQ=255)                                                                                                          cagGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCca                                       >  2:348933/1‑122 (MQ=255)                                                                                                          cagGGTTTCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACa                     >  2:1117167/1‑140 (MQ=255)                                                                                                                ttCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcac               <  2:191662/140‑1 (MQ=255)                                                                                                                 tCACATTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcacc              <  1:629262/140‑1 (MQ=255)                                                                                                                   acaTTGCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccat            <  1:616144/140‑1 (MQ=255)                                                                                                                        gCTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccatcacca       >  2:818480/1‑140 (MQ=255)                                                                                                                         cTGGAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccatcaccat      >  2:328694/1‑140 (MQ=255)                                                                                                                            gAGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCt   >  1:851486/1‑140 (MQ=255)                                                                                                                             aGGCCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCtt  <  2:933383/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                         AACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTT  >  NC_000913/3462254‑3462517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑