Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,598,550	A→G	intergenic (+183/+5)	panZ → / ← livJ	PanD maturation factor/branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,598,550	0	A	G	100.0%	314.7 / NA	86	intergenic (+183/+5)	panZ/livJ	PanD maturation factor/branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/41);  total (45/41)

TCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAT  >  NC_000913/3598416‑3598684                                                                                                                                       |                                                                                                                                       tCAGGCCTACAATGTGTGTTACAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTgatgat                                                                                                                                   <  1:1269161/140‑1 (MQ=255)     gCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACt                                                                                                                               >  1:1536456/1‑140 (MQ=255)        tACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCg                                                                                                                            >  1:305052/1‑140 (MQ=255)          cAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCa                                                                                                                          >  2:188705/1‑140 (MQ=255)           aaTGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCAt                                                                                                                         <  2:591629/140‑1 (MQ=255)              gtgtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCgg                                                                                                                      <  2:305052/140‑1 (MQ=255)               tgtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCAt                                                                                                                         >  2:363200/1‑136 (MQ=255)               tgtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCAt                                                                                                                         <  1:363200/136‑1 (MQ=255)               tgtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGt                                                                                                                     >  2:1533440/1‑140 (MQ=255)               tgtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGt                                                                                                                     >  1:446094/1‑140 (MQ=255)               tgtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGt                                                                                                                     >  1:1337138/1‑140 (MQ=255)               tgtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGt                                                                                                                     >  2:393595/1‑140 (MQ=255)               tgtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGt                                                                                                                     >  2:240827/1‑140 (MQ=255)                gtgtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTc                                                                                                                    >  1:950362/1‑140 (MQ=255)                  gtTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgc                                                                                                                  >  2:1505976/1‑140 (MQ=255)                   ttGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgcc                                                                                                                 >  1:784739/1‑140 (MQ=255)                    tGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATc                                                                                                                        <  2:348900/132‑1 (MQ=255)                    tGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATc                                                                                                                        >  1:348900/1‑132 (MQ=255)                    tGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgccg                                                                                                                <  1:188705/140‑1 (MQ=255)                    tGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgccg                                                                                                                >  1:756673/1‑140 (MQ=255)                    tGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgccg                                                                                                                <  2:1337138/140‑1 (MQ=255)                    tGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgccg                                                                                                                <  2:446094/140‑1 (MQ=255)                    tGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgccg                                                                                                                <  1:1505976/140‑1 (MQ=255)                      cAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgccgtg                                                                                                              >  1:159003/1‑140 (MQ=255)                         tttACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgccgtgccg                                                                                                           >  1:621600/1‑140 (MQ=255)                         tttACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCgccgtgccg                                                                                                           >  2:103184/1‑140 (MQ=255)                               gATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCg                                                                                                     <  1:240827/140‑1 (MQ=255)                                    cTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCa                                                                                                <  1:391378/140‑1 (MQ=255)                                    cTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCa                                                                                                <  1:103184/140‑1 (MQ=255)                                         gATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCaa                                                                                           <  2:214616/140‑1 (MQ=255)                                               gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGc                                                                                     >  2:2042/1‑140 (MQ=255)                                                 aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCg                                                                                   <  1:1533440/140‑1 (MQ=255)                                                   gCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGaa                                                                                 >  1:636837/1‑140 (MQ=255)                                                     cGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACt                                                                               <  2:1484203/140‑1 (MQ=255)                                                       gATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCa                                                                             >  2:409338/1‑140 (MQ=255)                                                       gATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCa                                                                             >  1:270754/1‑140 (MQ=255)                                                       gATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCa                                                                             >  2:1278612/1‑140 (MQ=255)                                                        aTAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaa                                                                            >  1:1015746/1‑140 (MQ=255)                                                        aTAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaa                                                                            >  2:1467172/1‑140 (MQ=255)                                                        aTAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGAGTGCCAGTCAAATACGCCGAACTCaa                                                                            >  1:709553/1‑140 (MQ=255)                                                         tAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCaaa                                                                           <  2:570365/140‑1 (MQ=255)                                                             gCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCt                                                                       >  2:982356/1‑140 (MQ=255)                                                             gCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCt                                                                       <  1:393595/140‑1 (MQ=255)                                                                 ttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCa                                                                   >  2:268074/1‑140 (MQ=255)                                                                 ttACGCCGCATCCGGCATGAAGCAACGTACTCGATATCAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCa                                                                   >  1:94298/1‑140 (MQ=255)                                                                   aCGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGa                                                                 <  2:159003/140‑1 (MQ=255)                                                                        gcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGcc                                                            <  2:636837/140‑1 (MQ=255)                                                                        gcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATAGcc                                                            <  1:1467172/140‑1 (MQ=255)                                                                         cATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCt                                                           >  1:1080479/1‑140 (MQ=255)                                                                         cATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCt                                                           >  1:1434502/1‑140 (MQ=255)                                                                          aTCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCtt                                                          >  2:514488/1‑140 (MQ=255)                                                                             cGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTtct                                                       <  2:393437/140‑1 (MQ=255)                                                                             cGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTtct                                                       <  2:1080479/140‑1 (MQ=255)                                                                             cGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTtct                                                       <  2:756673/140‑1 (MQ=255)                                                                             cGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTtct                                                       <  1:268074/140‑1 (MQ=255)                                                                                  tGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATcc                                                  <  2:784739/140‑1 (MQ=255)                                                                                  tGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATcc                                                  <  2:809969/140‑1 (MQ=255)                                                                                          cGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                          <  1:409338/140‑1 (MQ=255)                                                                                            tACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGc                                         >  1:1581992/1‑139 (MQ=255)                                                                                            tACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGc                                         <  2:1581992/139‑1 (MQ=255)                                                                                             aCTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                       <  2:1434502/140‑1 (MQ=255)                                                                                             aCTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                       <  2:621600/140‑1 (MQ=255)                                                                                             aCTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                       <  1:1173144/140‑1 (MQ=255)                                                                                              cTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGt                                      >  1:470772/1‑140 (MQ=255)                                                                                               tCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTc                                     <  1:514488/140‑1 (MQ=255)                                                                                                     ttAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTa                               >  1:1013160/1‑140 (MQ=255)                                                                                                      tAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                       >  2:283374/1‑131 (MQ=255)                                                                                                      tAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                       <  1:283374/131‑1 (MQ=255)                                                                                                      tAGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTAc                              >  1:544031/1‑140 (MQ=255)                                                                                                       aGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACg                             <  1:563296/140‑1 (MQ=255)                                                                                                       aGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACg                             >  2:563296/1‑140 (MQ=255)                                                                                                       aGCAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACg                             >  2:606913/1‑140 (MQ=255)                                                                                                         cAATTTGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGt                           <  2:1013160/140‑1 (MQ=255)                                                                                                             ttGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCa                                  >  1:735474/1‑129 (MQ=255)                                                                                                             ttGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCa                                  <  2:735474/129‑1 (MQ=255)                                                                                                             ttGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACgg                            >  1:823066/1‑135 (MQ=255)                                                                                                             ttGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACgg                            <  2:823066/135‑1 (MQ=255)                                                                                                             ttGGCGGCAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATcc                       >  2:576154/1‑140 (MQ=255)                                                                                                               ggcggcAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCAc                     >  2:1553585/1‑140 (MQ=255)                                                                                                                gcggcAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACg                    <  1:576154/140‑1 (MQ=255)                                                                                                                gcggcAACCCAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACg                    <  2:1536456/140‑1 (MQ=255)                                                                                                                       cccAAAGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCg             <  2:759337/140‑1 (MQ=255)                                                                                                                           aaGTTGCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCttt         <  1:982356/140‑1 (MQ=255)                                                                                                                                gCCGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGt    >  2:481642/1‑140 (MQ=255)                                                                                                                                 ccGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTa   >  2:770539/1‑140 (MQ=255)                                                                                                                                 ccGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTa   >  1:1540750/1‑140 (MQ=255)                                                                                                                                  cGATTGATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAt  <  2:26900/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TCAGGCCTACAATGTGTGTTGCAATTTACTGATTTCTTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAT  >  NC_000913/3598416‑3598684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑