Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,692,872	T→C	T789A (ACG→GCG)	bcsA ←	cellulose synthase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,692,872	0	T	C	100.0%	204.4 / NA	57	T789A (ACG→GCG)	bcsA	cellulose synthase catalytic subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/32);  total (25/32)

ACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCA  >  NC_000913/3692736‑3693003                                                                                                                                         |                                                                                                                                    aCCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGcaca                                                                                                                                  >  2:342022/1‑140 (MQ=255)   cGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACt                                                                                                                                >  1:827743/1‑140 (MQ=255)    gaagaaGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTg                                                                                                                               >  1:943996/1‑140 (MQ=255)        aagGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGcaca                                                                                                                           <  2:1513552/140‑1 (MQ=255)        aagGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGcaca                                                                                                                           >  2:1228998/1‑140 (MQ=255)          ggCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAATGCACACTGCACaaa                                                                                                                         >  2:987108/1‑140 (MQ=255)               aaaCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGa                                                                                                                    >  1:233302/1‑140 (MQ=255)                aaCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGat                                                                                                                   <  1:1097213/140‑1 (MQ=255)                aaCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGat                                                                                                                   <  2:575409/140‑1 (MQ=255)                aaCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGat                                                                                                                   <  1:180065/140‑1 (MQ=255)                 aCTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGata                                                                                                                  <  1:665505/140‑1 (MQ=255)                     cGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGtt                                                                                                              <  1:1102060/140‑1 (MQ=255)                     cGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGtt                                                                                                              >  1:569781/1‑140 (MQ=255)                      gCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATTGATATGTTg                                                                                                             <  1:1108755/140‑1 (MQ=255)                        cAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCt                                                                                                           <  2:161047/140‑1 (MQ=255)                           aTGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTggg                                                                                                        >  1:155114/1‑140 (MQ=255)                               cGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGggtggt                                                                                                    >  1:84807/1‑140 (MQ=255)                                        cGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCAt                                                                                           >  1:201812/1‑140 (MQ=255)                                         ggAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCAtt                                                                                          >  1:346992/1‑140 (MQ=255)                                              ccGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCg                                                                                               >  2:798031/1‑130 (MQ=255)                                              ccGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCg                                                                                               <  1:798031/130‑1 (MQ=255)                                              ccGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAAttt                                                                                     <  2:155114/140‑1 (MQ=255)                                               cGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTc                                                                                    >  2:1056030/1‑140 (MQ=255)                                                   cTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGcc                                                                                <  1:1579953/140‑1 (MQ=255)                                                   cTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACCCACACTGCATAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGcc                                                                                <  2:1190984/140‑1 (MQ=255)                                                    ttCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGccc                                                                               <  1:213549/140‑1 (MQ=255)                                                     tCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCa                                                                              <  1:1376242/140‑1 (MQ=255)                                                      cAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             <  2:233302/140‑1 (MQ=255)                                                      cAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             <  2:1392259/140‑1 (MQ=255)                                                      cAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             <  1:342022/140‑1 (MQ=255)                                                           tatCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCa                                                                        >  2:427820/1‑140 (MQ=255)                                                            atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             >  1:1511195/1‑134 (MQ=255)                                                            atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCaa                                                                             <  2:1511195/134‑1 (MQ=255)                                                            atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCAt                                                                       <  2:1175087/140‑1 (MQ=255)                                                            atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCAt                                                                       <  2:1213271/140‑1 (MQ=255)                                                            atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCAt                                                                       >  1:41931/1‑140 (MQ=255)                                                            atCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCAt                                                                       <  1:456972/140‑1 (MQ=255)                                                                agcagACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCACCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTAcc                                                                   >  2:283933/1‑140 (MQ=255)                                                                   agACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCAt                                                                >  1:1208431/1‑140 (MQ=255)                                                                     aCTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCa                                                              <  2:41931/140‑1 (MQ=255)                                                                       tttCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCAcg                                                            <  2:943996/140‑1 (MQ=255)                                                                               ggCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCAc                                                    >  1:1161690/1‑140 (MQ=255)                                                                                         ccGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCggg                                          <  1:1403544/140‑1 (MQ=255)                                                                                          cGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGa                                         <  1:987108/140‑1 (MQ=255)                                                                                            ggTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGTGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAg                                       >  2:1281265/1‑140 (MQ=255)                                                                                             gTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGa                                      <  1:280808/140‑1 (MQ=255)                                                                                               aGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACg                                    <  1:799921/140‑1 (MQ=255)                                                                                                 cTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTa                                  <  2:201812/140‑1 (MQ=255)                                                                                                 cTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTa                                  <  1:905023/140‑1 (MQ=255)                                                                                                           agagCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGa                        <  2:1482305/140‑1 (MQ=255)                                                                                                            gagCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGAc                       <  2:147526/140‑1 (MQ=255)                                                                                                            gagCGCCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGAc                       >  1:219525/1‑140 (MQ=255)                                                                                                              gcgcCCATGTATCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACcg                     >  2:649811/1‑140 (MQ=255)                                                                                                                        aTCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGTGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCa           <  1:1281265/140‑1 (MQ=255)                                                                                                                         tCCGCACGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCaa          >  1:511860/1‑140 (MQ=255)                                                                                                                              aCGGGCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGAt     >  2:911032/1‑140 (MQ=255)                                                                                                                                 ggCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCa  >  2:914260/1‑140 (MQ=255)                                                                                                                                 ggCAAACGCACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCa  >  2:951045/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    ACCGAAGAAGGCGCAAACTCCGCCAGATGGCGGTAGCCACGGAAGCCGAGCTTCAGAATATCCAGCAGACTTTCCAGCGGCTTATCTTCCGGGTAGCTGTCCTGCCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCA  >  NC_000913/3692736‑3693003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑