Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	400,681	A→G	G81G (GGT→GGC)	ddlA ←	D‑alanine‑‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	400,681	0	A	G	100.0%	318.2 / NA	88	G81G (GGT→GGC)	ddlA	D‑alanine‑‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/49);  total (39/49)

ACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACA  >  NC_000913/400545‑400810                                                                                                                                         |                                                                                                                                  aCGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCt                                                                                                                                <  2:75917/140‑1 (MQ=255)   ggTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGTTCATGTTTGCCTgg                                                                                                                              <  1:464142/140‑1 (MQ=255)   ggTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTgg                                                                                                                              <  1:255064/140‑1 (MQ=255)     tAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCa                                                                                                                            >  1:813708/1‑140 (MQ=255)     tAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCa                                                                                                                            >  1:1442863/1‑140 (MQ=255)      aaaTTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCAc                                                                                                                           <  2:813708/140‑1 (MQ=255)      aaaTTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCAc                                                                                                                           >  2:794375/1‑140 (MQ=255)      aaaTTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCAc                                                                                                                           <  1:451083/140‑1 (MQ=255)      aaaTTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCAc                                                                                                                           <  2:461560/140‑1 (MQ=255)      aaaTTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCAc                                                                                                                           >  1:822362/1‑140 (MQ=255)             cGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACAGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCa                                                                                                                    >  1:885833/1‑140 (MQ=255)               aCCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAg                                                                                                                  >  2:117706/1‑140 (MQ=255)               aCCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAg                                                                                                                  >  1:681851/1‑140 (MQ=255)               aCCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAg                                                                                                                  >  2:1107566/1‑140 (MQ=255)                     agcaTTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGt                                                                                                            <  2:1308051/140‑1 (MQ=255)                       caTTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCg                                                                                                          <  1:794375/140‑1 (MQ=255)                       caTTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCg                                                                                                          <  2:197581/140‑1 (MQ=255)                             cTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAg                                                                                                    >  2:415659/1‑140 (MQ=255)                               gCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAggg                                                                                                  >  1:190802/1‑140 (MQ=255)                               gCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACAGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAggg                                                                                                  <  2:885833/140‑1 (MQ=255)                                 aaGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGgcg                                                                                                >  2:1556602/1‑140 (MQ=255)                                 aaGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGgcg                                                                                                >  2:762224/1‑140 (MQ=255)                                 aaGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGgcg                                                                                                <  2:1110993/140‑1 (MQ=255)                                  aGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGgcgc                                                                                               <  2:822362/140‑1 (MQ=255)                                   ggAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgca                                                                                              >  1:683342/1‑140 (MQ=255)                                     aaCCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaac                                                                                            <  1:503561/140‑1 (MQ=255)                                     aaCCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaac                                                                                            <  2:1134484/140‑1 (MQ=255)                                      aCCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaacg                                                                                           >  2:1058074/1‑140 (MQ=255)                                      aCCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaacg                                                                                           <  1:415659/140‑1 (MQ=255)                                      aCCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaacg                                                                                           >  2:424638/1‑140 (MQ=255)                                      aCCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaacg                                                                                           >  1:715501/1‑140 (MQ=255)                                         aTCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaacgcaa                                                                                        >  2:925339/1‑140 (MQ=255)                                          tCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       <  1:1107566/140‑1 (MQ=255)                                          tCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       <  2:1442863/140‑1 (MQ=255)                                          tCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       >  1:1136184/1‑140 (MQ=255)                                          tCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       <  2:294237/140‑1 (MQ=255)                                          tCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       >  1:294237/1‑140 (MQ=255)                                          tCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       <  2:681851/140‑1 (MQ=255)                                          tCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       <  2:715501/140‑1 (MQ=255)                                          tCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       <  1:88906/140‑1 (MQ=255)                                           cTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAata                                                                                      <  2:190802/140‑1 (MQ=255)                                               gctgggCGAACCGTGGACAATCGGGAAAATGACATCCTCCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTAGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       <  2:1499541/130‑1 (MQ=255)                                                   aGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAgg                                                                              <  2:486051/140‑1 (MQ=255)                                                   aGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCCGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCTGAAGGGCGCAACGCAATATGGGCAgg                                                                              <  1:428274/140‑1 (MQ=255)                                                     cGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGAt                                                                            >  2:787758/1‑140 (MQ=255)                                                     cGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGAt                                                                            <  1:138711/140‑1 (MQ=255)                                                     cGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGAt                                                                            <  1:117706/140‑1 (MQ=255)                                                     cGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGAt                                                                            >  2:210797/1‑140 (MQ=255)                                                     cGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGAt                                                                            <  1:1116068/140‑1 (MQ=255)                                                      gTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       <  1:289022/128‑1 (MQ=255)                                                      gTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAat                                                                                       >  2:289022/1‑128 (MQ=255)                                                          cGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCt                                                                       >  1:697869/1‑140 (MQ=255)                                                           gTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCt                                                                       <  2:550329/139‑1 (MQ=255)                                                           gTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCt                                                                       >  1:550329/1‑139 (MQ=255)                                                               aCAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCAtt                                                                  >  2:1403214/1‑140 (MQ=255)                                                               aCAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCAtt                                                                  >  1:1338431/1‑140 (MQ=255)                                                               aCAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCAtt                                                                  >  2:369871/1‑140 (MQ=255)                                                                   tCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGc                                                              <  1:210797/140‑1 (MQ=255)                                                                   tCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGc                                                              >  2:1212414/1‑140 (MQ=255)                                                                   tCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGc                                                              <  2:697869/140‑1 (MQ=255)                                                                   tCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGc                                                              >  1:330348/1‑140 (MQ=255)                                                                   tCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGc                                                              >  1:304953/1‑140 (MQ=255)                                                                    cGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCa                                                             >  1:573980/1‑140 (MQ=255)                                                                            tGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTg                                                     <  1:1556602/140‑1 (MQ=255)                                                                             gACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGc                                                    >  2:202187/1‑140 (MQ=255)                                                                                   cACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCAt                                              <  1:769777/140‑1 (MQ=255)                                                                                        tCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTg                                         >  1:1400851/1‑140 (MQ=255)                                                                                          ggCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGAc                                       >  2:322399/1‑140 (MQ=255)                                                                                             aaCGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTg                                    >  1:518594/1‑140 (MQ=255)                                                                                              aCGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGc                                   <  1:1058074/140‑1 (MQ=255)                                                                                                ggCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCa                                 <  1:956529/140‑1 (MQ=255)                                                                                                ggCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCa                                 <  2:304953/140‑1 (MQ=255)                                                                                                ggCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCa                                 <  2:1136184/140‑1 (MQ=255)                                                                                                  cTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCAtt                               >  2:760875/1‑140 (MQ=255)                                                                                                      ccGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGccc                           <  1:30353/140‑1 (MQ=255)                                                                                                      ccGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGccc                           <  2:86188/140‑1 (MQ=255)                                                                                                      ccGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGccc                           <  2:330348/140‑1 (MQ=255)                                                                                                      ccGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGccc                           <  1:202187/140‑1 (MQ=255)                                                                                                      ccGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGccc                           <  1:517672/140‑1 (MQ=255)                                                                                                         ttttGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTg                        <  1:1212414/140‑1 (MQ=255)                                                                                                           ttGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGtt                      >  2:161845/1‑140 (MQ=255)                                                                                                           ttGCGCGTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGtt                      <  2:518594/140‑1 (MQ=255)                                                                                                              cgcgTCGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTAt                   <  1:412720/140‑1 (MQ=255)                                                                                                                   cGATAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATg              <  1:247815/140‑1 (MQ=255)                                                                                                                     aTAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCGATGcc            <  2:456588/140‑1 (MQ=255)                                                                                                                     aTAAGCTGATGCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGcc            <  1:382468/140‑1 (MQ=255)                                                                                                                               gCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGcaca  >  2:180441/1‑140 (MQ=255)                                                                                                                               gCTCATGTTTGCCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGcaca  >  2:896863/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  ACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACA  >  NC_000913/400545‑400810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑