Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,859,745	G→A	C124C (TGC→TGT)	yidK ←	putative transporter YidK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,859,745	0	G	A	100.0%	213.9 / NA	60	C124C (TGC→TGT)	yidK	putative transporter YidK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/35);  total (25/35)

GCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGT  >  NC_000913/3859609‑3859876                                                                                                                                         |                                                                                                                                    gCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAg                                                                                                                                  <  2:798274/140‑1 (MQ=255)   aaaaTTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGaa                                                                                                                                <  1:1140790/140‑1 (MQ=255)      aTTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTc                                                                                                                             <  1:593094/140‑1 (MQ=255)      aTTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTc                                                                                                                             <  1:1272586/140‑1 (MQ=255)        tACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCga                                                                                                                           <  1:1191123/140‑1 (MQ=255)        tACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCga                                                                                                                           <  1:1474312/140‑1 (MQ=255)         aCCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCgat                                                                                                                          <  2:626418/140‑1 (MQ=255)           cAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCgatga                                                                                                                        >  2:1286700/1‑140 (MQ=255)               aGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGataat                                                                                                                    >  2:1558494/1‑140 (MQ=255)               aGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGataat                                                                                                                    >  1:288396/1‑140 (MQ=255)               aGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGataat                                                                                                                    >  2:1411783/1‑140 (MQ=255)               aGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGataat                                                                                                                    >  1:698126/1‑140 (MQ=255)                   aGATAGCCGCACCGTGAGAAATCTGTAGCGATTGCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATAcgc                                                                                                                <  2:1051373/140‑1 (MQ=255)                    gATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATAcgcg                                                                                                               <  1:1411783/140‑1 (MQ=255)                         ccGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGcgtcgt                                                                                                           <  1:849138/139‑1 (MQ=255)                         ccGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGcgtcgt                                                                                                           >  2:849138/1‑139 (MQ=255)                         ccGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGtt                                                                                                          <  1:1462335/140‑1 (MQ=255)                         ccGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGtt                                                                                                          <  1:191966/140‑1 (MQ=255)                         ccGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGtt                                                                                                          <  1:535937/140‑1 (MQ=255)                          cGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGttt                                                                                                         >  2:1274094/1‑140 (MQ=255)                          cGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGttt                                                                                                         <  2:1529051/140‑1 (MQ=255)                              ccGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATc                                                                                                     >  2:462505/1‑140 (MQ=255)                               cGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCa                                                                                                    >  1:860970/1‑140 (MQ=255)                                gTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCAt                                                                                                   <  2:174845/140‑1 (MQ=255)                                       aTCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtc                                                                                            <  2:288396/140‑1 (MQ=255)                                          tGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtcctc                                                                                         >  1:1211126/1‑140 (MQ=255)                                           gTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATAGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtcctcc                                                                                        >  2:1434196/1‑140 (MQ=255)                                            tAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCa                                                                                       <  1:1434196/140‑1 (MQ=255)                                              gCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGa                                                                                     <  2:376445/140‑1 (MQ=255)                                                  ttCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAAt                                                                                 >  1:1134747/1‑140 (MQ=255)                                                   tCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATc                                                                                >  2:573627/1‑140 (MQ=255)                                                   tCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATc                                                                                <  1:834270/140‑1 (MQ=255)                                                   tCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATc                                                                                >  1:1444181/1‑140 (MQ=255)                                                    ccccGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCg                                                                               >  2:140578/1‑140 (MQ=255)                                                    ccccGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCg                                                                               <  1:1558494/140‑1 (MQ=255)                                                       cGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCgggg                                                                            <  1:1286700/140‑1 (MQ=255)                                                         aCGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGAt                                                                          <  2:1134747/140‑1 (MQ=255)                                                         aCGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGAt                                                                          <  1:462505/140‑1 (MQ=255)                                                              aaaCAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGAtggtgg                                                                     >  2:1322367/1‑140 (MQ=255)                                                                     cTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                              <  2:1211126/140‑1 (MQ=255)                                                                     cTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                              <  1:923825/140‑1 (MQ=255)                                                                     cTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                              <  1:1322367/140‑1 (MQ=255)                                                                       gTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCcgc                                                            <  2:1487634/140‑1 (MQ=255)                                                                        ttGAGCGCCAACGCACCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCcgcg                                                           >  2:1182152/1‑140 (MQ=255)                                                                              gcCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAg                                                     >  2:536129/1‑140 (MQ=255)                                                                                    gcgcCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAgcg                                               <  2:93017/140‑1 (MQ=255)                                                                                     cgcCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAgcgc                                              <  1:536129/140‑1 (MQ=255)                                                                                      gcCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGcgcg                                             <  2:698126/140‑1 (MQ=255)                                                                                                     aaTCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcg                              >  1:1138947/1‑140 (MQ=255)                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGcgcg                            >  2:1261640/1‑140 (MQ=255)                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGcgcg                            >  2:1247191/1‑140 (MQ=255)                                                                                                            aGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcg                              >  2:988569/1‑133 (MQ=255)                                                                                                            aGAAAGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcg                              <  1:988569/133‑1 (MQ=255)                                                                                                              aaaGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGa                     >  1:94965/1‑140 (MQ=255)                                                                                                               aaGCAGACGCCGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGAt                    <  1:1274094/140‑1 (MQ=255)                                                                                                                        ccGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAg                          <  1:86397/125‑1 (MQ=255)                                                                                                                        ccGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAg                          >  2:86397/1‑125 (MQ=255)                                                                                                                        ccGGTGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCAc           >  1:1462027/1‑140 (MQ=255)                                                                                                                            tGGCAATTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCt       <  1:1261640/140‑1 (MQ=255)                                                                                                                                 aTTAGGAAACAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGt  <  2:860970/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAGT  >  NC_000913/3859609‑3859876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑