Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,932,121	A→G	N269S (AAC→AGC)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,121	0	A	G	100.0%	338.5 / NA	94	N269S (AAC→AGC)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (59/35);  total (59/35)

GTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACG  >  NC_000913/3931984‑3932251                                                                                                                                          |                                                                                                                                   gtCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGcc                                                                                                                                  >  1:674390/1‑140 (MQ=255)     aTTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGa                                                                                                                              <  2:576237/140‑1 (MQ=255)       tACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAg                                                                                                                            <  2:1371184/140‑1 (MQ=255)        aCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGc                                                                                                                           <  1:891880/140‑1 (MQ=255)           ggggTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGAt                                                                                                                        <  1:541758/140‑1 (MQ=255)            gggTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTACCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAGAGAGCCCGGAAGCGAtt                                                                                                                       >  2:1428335/1‑140 (MQ=255)              gTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTaa                                                                                                                     >  2:809739/1‑140 (MQ=255)               tCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAg                                                                                                                    >  2:1359491/1‑140 (MQ=255)                  aGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAAc                                                                                                                 >  2:685483/1‑140 (MQ=255)                  aGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAAc                                                                                                                 <  1:685483/140‑1 (MQ=255)                     cgcTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCg                                                                                                              <  2:768656/140‑1 (MQ=255)                        tGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGttc                                                                                                           >  2:1317798/1‑140 (MQ=255)                            tGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTcc                                                                                                       >  2:1548415/1‑140 (MQ=255)                             gCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCt                                                                                                      >  2:389962/1‑140 (MQ=255)                             gCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCt                                                                                                      >  2:819601/1‑140 (MQ=255)                             gCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCt                                                                                                      >  2:919671/1‑140 (MQ=255)                               tGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGt                                                                                                    <  1:1548415/140‑1 (MQ=255)                               tGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGt                                                                                                    <  1:389962/140‑1 (MQ=255)                                 atatGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTg                                                                                                  <  1:1428335/140‑1 (MQ=255)                                    tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCa                                                                                               >  2:1468809/1‑140 (MQ=255)                                    tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCa                                                                                               >  2:1038853/1‑140 (MQ=255)                                    tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCa                                                                                               >  2:1030991/1‑140 (MQ=255)                                    tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCa                                                                                               >  1:1526237/1‑140 (MQ=255)                                      gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCAcc                                                                                             >  1:335840/1‑140 (MQ=255)                                      gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCAcc                                                                                             >  2:355184/1‑140 (MQ=255)                                      gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCAcc                                                                                             <  1:819601/140‑1 (MQ=255)                                         cACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGa                                                                                          >  2:36756/1‑140 (MQ=255)                                           cTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACt                                                                                        >  2:699525/1‑140 (MQ=255)                                              tGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTggg                                                                                     >  2:1322343/1‑140 (MQ=255)                                                gTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGCCTGGgcg                                                                                   <  2:335840/140‑1 (MQ=255)                                                  aaGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCt                                                                                 <  1:721637/140‑1 (MQ=255)                                                    gTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGa                                                                               <  1:809739/140‑1 (MQ=255)                                                      tCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATc                                                                             >  2:285987/1‑140 (MQ=255)                                                      tCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATc                                                                             >  1:67010/1‑140 (MQ=255)                                                      tCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCC‑GAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATcc                                                                            >  1:1535481/1‑140 (MQ=255)                                                          tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATcc                                                                            <  2:1055715/137‑1 (MQ=255)                                                          tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATcc                                                                            >  1:1055715/1‑137 (MQ=255)                                                          tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCgc                                                                         >  2:715199/1‑140 (MQ=255)                                                          tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCgc                                                                         >  1:145654/1‑140 (MQ=255)                                                             tCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCgctgc                                                                      >  2:1384448/1‑140 (MQ=255)                                                               gCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATcc                                                                            >  2:205533/1‑132 (MQ=255)                                                               gCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATcc                                                                            <  1:205533/132‑1 (MQ=255)                                                               gCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGctgctg                                                                    >  1:171343/1‑140 (MQ=255)                                                               gCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGctgctg                                                                    >  2:42879/1‑140 (MQ=255)                                                                 cTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGat                                                                  <  1:1317798/140‑1 (MQ=255)                                                                    gCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCTCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGatcat                                                               >  2:537751/1‑140 (MQ=255)                                                                    gCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGatcat                                                               >  2:564698/1‑140 (MQ=255)                                                                          ttCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATcgccgc                                                         <  1:1468809/140‑1 (MQ=255)                                                                              ccGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTg                                                     >  1:854017/1‑140 (MQ=255)                                                                               cgtcgtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACGGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTgg                                                    >  2:377784/1‑140 (MQ=255)                                                                                gtcgtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGc                                                   <  2:674390/140‑1 (MQ=255)                                                                                 tcgtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCg                                                  <  1:285987/140‑1 (MQ=255)                                                                                    tATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACg                                               >  1:16938/1‑140 (MQ=255)                                                                                    tATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACg                                               >  2:1499953/1‑140 (MQ=255)                                                                                         ccttccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAAt                                          >  2:960685/1‑140 (MQ=255)                                                                                            tccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCGCCGGACTGGGTGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGc                                       >  2:392301/1‑140 (MQ=255)                                                                                            tccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCa                                                        >  1:22012/1‑123 (MQ=255)                                                                                            tccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCa                                                        <  2:22012/123‑1 (MQ=255)                                                                                             ccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGcc                                      >  2:1216333/1‑140 (MQ=255)                                                                                                    ccTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAgg                               <  1:1359491/140‑1 (MQ=255)                                                                                                    ccTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAgg                               <  2:145654/140‑1 (MQ=255)                                                                                                      ttAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCg                             <  2:854017/140‑1 (MQ=255)                                                                                                      ttAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCg                             >  2:509260/1‑140 (MQ=255)                                                                                                       tAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAgg                               <  2:509647/137‑1 (MQ=255)                                                                                                       tAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAgg                               >  1:509647/1‑137 (MQ=255)                                                                                                       tAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCgg                            >  2:1235545/1‑140 (MQ=255)                                                                                                        aaTTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGt                           <  1:1322343/140‑1 (MQ=255)                                                                                                             cTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGt                           >  2:1387352/1‑135 (MQ=255)                                                                                                             cTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGt                           <  1:1387352/135‑1 (MQ=255)                                                                                                              ttCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTAtctc                     >  1:166511/1‑140 (MQ=255)                                                                                                                cGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTg                   >  2:1552618/1‑140 (MQ=255)                                                                                                                 ggTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTgg                  <  1:715199/140‑1 (MQ=255)                                                                                                                 ggTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTgg                  <  1:919671/140‑1 (MQ=255)                                                                                                                  gTCAGGGAGCTCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGc                 <  1:537751/140‑1 (MQ=255)                                                                                                                  gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCGCCGGACTGGGTGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGc                 <  1:392301/140‑1 (MQ=255)                                                                                                                  gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATctct                    <  2:1497220/137‑1 (MQ=255)                                                                                                                  gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATctct                    >  1:1497220/1‑137 (MQ=255)                                                                                                                  gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGc                 >  2:299229/1‑140 (MQ=255)                                                                                                                  gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGc                 <  1:564698/140‑1 (MQ=255)                                                                                                                  gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCAGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGc                 >  1:569897/1‑140 (MQ=255)                                                                                                                      gggAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGtct             >  1:236485/1‑140 (MQ=255)                                                                                                                       ggAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGt                           >  2:1555606/1‑125 (MQ=255)                                                                                                                       ggAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGt                           <  1:1555606/125‑1 (MQ=255)                                                                                                                           cgcTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCa        >  1:1575351/1‑140 (MQ=255)                                                                                                                           cgcTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCa        >  1:1309826/1‑140 (MQ=255)                                                                                                                            gcTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCAt       >  2:455480/1‑140 (MQ=255)                                                                                                                             cTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCAtt      >  1:1582499/1‑140 (MQ=255)                                                                                                                              tgttgtTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTg     <  2:1526237/140‑1 (MQ=255)                                                                                                                               gttgttAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGa    <  2:999065/140‑1 (MQ=255)                                                                                                                                ttgttAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGAc   >  1:196576/1‑140 (MQ=255)                                                                                                                                 tgttAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGAcg  >  2:575922/1‑140 (MQ=255)                                                                                                                                 tgttAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGAcg  >  2:1103012/1‑140 (MQ=255)                                                                                                                                 tgttAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGAcg  >  1:939020/1‑140 (MQ=255)                                                                                                                                 tgttAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGGCTTTTCATTGTcg  >  1:1478468/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   GTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACG  >  NC_000913/3931984‑3932251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑