Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,056,607	C→T	intergenic (‑268/+18)	glnL ← / ← glnA	sensory histidine kinase NtrB/glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,056,607	0	C	T	100.0%	284.0 / NA	79	intergenic (‑268/+18)	glnL/glnA	sensory histidine kinase NtrB/glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/43);  total (36/43)

CGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCA  >  NC_000913/4056474‑4056739                                                                                                                                      |                                                                                                                                     cGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGc                                                                                                                                <  2:1368804/140‑1 (MQ=255)      gagaGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTa                                                                                                                           <  1:1157442/140‑1 (MQ=255)             aCTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACt                                                                                                                    >  1:1042381/1‑140 (MQ=255)              cTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACtt                                                                                                                   >  2:1361937/1‑140 (MQ=255)                 aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                <  1:428438/140‑1 (MQ=255)                 aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                <  2:1285681/140‑1 (MQ=255)                 aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                <  1:1199016/140‑1 (MQ=255)                 aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                <  2:533796/140‑1 (MQ=255)                 aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                <  1:124825/140‑1 (MQ=255)                 aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                <  2:141353/140‑1 (MQ=255)                   cTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                >  1:194298/1‑138 (MQ=255)                   cTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                <  2:194298/138‑1 (MQ=255)                   cTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACg                                                                                                              <  1:1365638/140‑1 (MQ=255)                    tGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGc                                                                                                             <  1:321469/140‑1 (MQ=255)                      cTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                >  2:515344/1‑135 (MQ=255)                      cTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                <  1:515344/135‑1 (MQ=255)                      cTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTg                                                                                                           >  2:705482/1‑140 (MQ=255)                        ccGGCCTACCACATCATCA‑CGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTgtag                                                                                                        >  1:135159/1‑140 (MQ=255)                           gCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACg                                                                                                              >  1:950952/1‑132 (MQ=255)                           gCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACg                                                                                                              <  2:950952/132‑1 (MQ=255)                           gCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTgtagta                                                                                                      >  2:1240804/1‑140 (MQ=255)                           gCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTgtagta                                                                                                      >  2:1000561/1‑140 (MQ=255)                           gCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTgtagta                                                                                                      <  1:1030488/140‑1 (MQ=255)                            ccTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTAc                                                                                                     >  2:1051460/1‑140 (MQ=255)                                ccACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                >  1:1359305/1‑125 (MQ=255)                                ccACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGa                                                                                                                <  2:1359305/125‑1 (MQ=255)                                 cacaTCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTc                                                                                                <  1:641074/140‑1 (MQ=255)                                    atcatcACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCaaa                                                                                             <  2:62720/140‑1 (MQ=255)                                    atcatcACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCaaa                                                                                             <  1:91419/140‑1 (MQ=255)                                      catcaCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAAct                                                                                           >  1:1297754/1‑140 (MQ=255)                                      catcaCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAAct                                                                                           >  1:186436/1‑140 (MQ=255)                                         caCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTa                                                                                        <  2:900201/140‑1 (MQ=255)                                          aCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTAc                                                                                       >  1:295100/1‑140 (MQ=255)                                          aCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTAc                                                                                       <  1:1240804/140‑1 (MQ=255)                                           ccGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTAcc                                                                                      <  1:1486595/140‑1 (MQ=255)                                              tgtAGGCCGGATAAGACGCATTTGCGTCGCATCCNGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGa                                                                                   >  1:222301/1‑140 (MQ=255)                                              tgtAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGa                                                                                   >  2:1501350/1‑140 (MQ=255)                                              tgtAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGa                                                                                   >  2:479342/1‑140 (MQ=255)                                              tgtAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGa                                                                                   >  2:961731/1‑140 (MQ=255)                                              tgtAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGa                                                                                   >  1:960837/1‑140 (MQ=255)                                                tAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATg                                                                                 <  1:1573407/140‑1 (MQ=255)                                                tAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATg                                                                                 >  1:1158991/1‑140 (MQ=255)                                                tAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATg                                                                                 >  1:1402550/1‑140 (MQ=255)                                                 aGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGc                                                                                <  1:961731/140‑1 (MQ=255)                                                         tAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCAt                                                                        <  1:1501350/140‑1 (MQ=255)                                                             aCGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgc                                                                    <  1:1281989/140‑1 (MQ=255)                                                             aCGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgc                                                                    >  1:446496/1‑140 (MQ=255)                                                              cGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgca                                                                   <  1:254504/140‑1 (MQ=255)                                                              cGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgca                                                                   <  1:1383961/140‑1 (MQ=255)                                                                cATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgcacg                                                                 <  2:295100/140‑1 (MQ=255)                                                                cATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgcacg                                                                 <  2:1158991/140‑1 (MQ=255)                                                                               cGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTcg                                                  <  2:135159/140‑1 (MQ=255)                                                                                      aTGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCa                                           <  1:1051460/140‑1 (MQ=255)                                                                                        gtgCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCaga                                         <  1:705482/140‑1 (MQ=255)                                                                                         tgCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGTATCCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCagag                                        <  2:1297754/140‑1 (MQ=255)                                                                                         tgCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCagag                                        <  2:934302/140‑1 (MQ=255)                                                                                         tgCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCagag                                        <  2:446496/140‑1 (MQ=255)                                                                                              gCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATg                                   <  2:1042381/140‑1 (MQ=255)                                                                                              gCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATg                                   <  1:660234/140‑1 (MQ=255)                                                                                              gCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATg                                   <  2:960837/140‑1 (MQ=255)                                                                                              gCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATg                                   >  1:1290175/1‑140 (MQ=255)                                                                                                 aCCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTAc                                >  1:722939/1‑140 (MQ=255)                                                                                                 aCCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTAc                                <  2:1402550/140‑1 (MQ=255)                                                                                                     aTCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCAt                            >  2:1298379/1‑140 (MQ=255)                                                                                                          aGGCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAAtt                       >  2:700925/1‑140 (MQ=255)                                                                                                            gCCTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGc                     <  1:1298379/140‑1 (MQ=255)                                                                                                              cTGCCAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCtt                   <  1:1361937/140‑1 (MQ=255)                                                                                                                 ccAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCGATTGCTTCGt                >  2:206017/1‑140 (MQ=255)                                                                                                                 ccAGAGACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGt                >  2:1189226/1‑140 (MQ=255)                                                                                                                   agagACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCa              <  2:186436/140‑1 (MQ=255)                                                                                                                   agagACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCa              >  1:1328047/1‑140 (MQ=255)                                                                                                                   agagACAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCa              <  2:1290175/140‑1 (MQ=255)                                                                                                                     agaCAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGt            >  1:437939/1‑140 (MQ=255)                                                                                                                      gaCAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTg           >  1:1163838/1‑140 (MQ=255)                                                                                                                      gaCAGGCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTg           >  1:718369/1‑140 (MQ=255)                                                                                                                          ggCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAaca       >  1:613625/1‑140 (MQ=255)                                                                                                                           gCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAacac      >  1:651008/1‑140 (MQ=255)                                                                                                                           gCGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAacac      >  2:974548/1‑140 (MQ=255)                                                                                                                            cGAAAAGTTTTCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACg     >  2:760291/1‑140 (MQ=255)                                                                                                                                   ttttCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCa  <  2:906883/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     CGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCA  >  NC_000913/4056474‑4056739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑