Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,541,175	(A)7→6	coding (219/603 nt)	fimB →	regulator for fimA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,541,169	0	A	.	100.0%	338.3 / NA	79	coding (213/603 nt)	fimB	regulator for fimA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (50/29);  total (50/29)

CAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCT  >  NC_000913/4541030‑4541303                                                                                                                                            |                                                                                                                                       caAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTa                                                                                                                                        >  1:811923/1‑140 (MQ=255) caAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTa                                                                                                                                        >  2:111567/1‑140 (MQ=255)      aCCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTaaaaaa                                                                                                                                   <  2:500806/140‑1 (MQ=255)          ggCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTaaaaaaggct                                                                                                                               >  1:938181/1‑136 (MQ=255)            ccTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCttt                                                                                                                            >  2:1041358/1‑140 (MQ=255)               cATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTca                                                                                                                         <  1:492854/140‑1 (MQ=255)                aTGCAGCACGTAATTATTGTCTGACTTTTCTTTGTTTTATTCATGGATTCCGGGCGAGTGAAATTTGTCGATTGTGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTcaa                                                                                                                        >  2:872389/1‑140 (MQ=255)                 tGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTcaac                                                                                                                       >  2:522597/1‑140 (MQ=255)                 tGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTcaac                                                                                                                       >  2:481219/1‑140 (MQ=255)                    agcaCGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCaacaac                                                                                                                    <  1:769414/140‑1 (MQ=255)                     gcaCGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACg                                                                                                                   >  1:786872/1‑140 (MQ=255)                      caCGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGc                                                                                                                  >  2:714293/1‑140 (MQ=255)                        cGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCAc                                                                                                                <  1:481219/140‑1 (MQ=255)                        cGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCAc                                                                                                                <  1:522597/140‑1 (MQ=255)                          tAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTc                                                                                                                          <  1:133335/128‑1 (MQ=255)                          tAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTc                                                                                                                          >  2:133335/1‑128 (MQ=255)                          tAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCAccc                                                                                                              >  1:657558/1‑140 (MQ=255)                           aaTTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCg                                                                                                             <  2:938181/140‑1 (MQ=255)                           aaTTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCg                                                                                                             <  1:892938/140‑1 (MQ=255)                                     tGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATa                                                                                                   >  2:999632/1‑140 (MQ=255)                                       aCTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTa                                                                                                          >  2:955405/1‑131 (MQ=255)                                       aCTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTa                                                                                                          <  2:781399/131‑1 (MQ=255)                                       aCTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTa                                                                                                          >  1:781399/1‑131 (MQ=255)                                       aCTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTa                                                                                                          <  1:955405/131‑1 (MQ=255)                                       aCTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATaaa                                                                                                 <  2:498951/140‑1 (MQ=255)                                             ctttgTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCg                                                                                                             >  2:1003245/1‑122 (MQ=255)                                             ctttgTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCg                                                                                                             <  1:1003245/122‑1 (MQ=255)                                                       ttCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTaa                                                                                 >  2:364235/1‑140 (MQ=255)                                                         cATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAGGTTCAGGCTTTaaaa                                                                               <  2:814398/140‑1 (MQ=255)                                                            ggTTTCCGGGCGAGTGAAATTTGTCGATTGGGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAAc                                                                            >  2:418069/1‑140 (MQ=255)                                                            ggTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑GAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAAc                                                                            >  2:974857/1‑140 (MQ=255)                                                                    ggCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAg                                                                    <  1:999632/140‑1 (MQ=255)                                                                        aGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTaaaaaa                                                                             <  1:213366/127‑1 (MQ=255)                                                                        aGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTaaaaaa                                                                             >  2:213366/1‑127 (MQ=255)                                                                        aGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAg                                                                    >  2:63560/1‑136 (MQ=255)                                                                        aGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAg                                                                    <  1:63560/136‑1 (MQ=255)                                                                          tGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCg                                                              <  2:657558/140‑1 (MQ=255)                                                                                       ttGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCg                                                 <  2:786872/140‑1 (MQ=255)                                                                                       ttGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCg                                                 <  2:811923/140‑1 (MQ=255)                                                                                         gAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑GAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCa                                               <  1:974857/140‑1 (MQ=255)                                                                                           ggATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATg                                             <  2:76173/140‑1 (MQ=255)                                                                                                 cGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTGCCCGCATGCTgaga                                       <  2:189306/140‑1 (MQ=255)                                                                                                 cGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTgaga                                       >  1:179674/1‑140 (MQ=255)                                                                                                    atatTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCg                                    <  2:1228539/140‑1 (MQ=255)                                                                                                      atTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTAcc                                                   >  1:960488/1‑123 (MQ=255)                                                                                                      atTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTAcc                                                   <  2:960488/123‑1 (MQ=255)                                                                                                      atTGATCTTAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAg                                  <  1:111567/140‑1 (MQ=255)                                                                                                              tAAGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCACGCTGAGAGCGAGTGGGTAtt                          >  1:1035930/1‑140 (MQ=255)                                                                                                               aaGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTAttt                         <  2:179674/140‑1 (MQ=255)                                                                                                               aaGGCAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTAttt                         >  1:291921/1‑140 (MQ=255)                                                                                                                   cAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTAt                     <  1:545895/140‑1 (MQ=255)                                                                                                                   cAAAGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTAt                     <  1:963093/140‑1 (MQ=255)                                                                                                                      aGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTAt                     >  1:611942/1‑137 (MQ=255)                                                                                                                      aGTGTATATATATCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTAt                     <  2:611942/137‑1 (MQ=255)                                                                                                                            tatatatCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTaa              >  2:832956/1‑138 (MQ=255)                                                                                                                            tatatatCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTaa              <  1:832956/138‑1 (MQ=255)                                                                                                                                 atCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAAt       >  1:290203/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  2:737250/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  1:1045087/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  1:155724/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  2:822616/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  1:31791/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  1:452414/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  1:541160/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  2:706845/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  2:647683/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  2:455597/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  2:479485/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  1:971247/1‑140 (MQ=255)                                                                                                                                  tCCATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATc      >  2:1068444/1‑140 (MQ=255)                                                                                                                                    cATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTAt                     <  1:284414/123‑1 (MQ=255)                                                                                                                                    cATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTAt                     >  2:284414/1‑123 (MQ=255)                                                                                                                                    cATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCg    >  2:1198282/1‑140 (MQ=255)                                                                                                                                    cATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCg    >  1:815490/1‑140 (MQ=255)                                                                                                                                    cATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCg    >  2:746332/1‑140 (MQ=255)                                                                                                                                    cATCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCg    >  2:1082056/1‑140 (MQ=255)                                                                                                                                     aTCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCACATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGc   >  1:1195911/1‑140 (MQ=255)                                                                                                                                      tCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCt  >  1:377476/1‑140 (MQ=255)                                                                                                                                      tCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCt  >  2:5172/1‑140 (MQ=255)                                                                                                                                      tCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCt  >  2:1097664/1‑140 (MQ=255)                                                                                                                                      tCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCt  >  2:82147/1‑140 (MQ=255)                                                                                                                                      tCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCt  >  2:507877/1‑140 (MQ=255)                                                                                                                                      tCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCt  >  1:994790/1‑140 (MQ=255)                                                                                                                                      tCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCt  >  1:251462/1‑140 (MQ=255)                                                                                                                                      tCGATT‑AAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCt  >  1:701700/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       CAAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCT  >  NC_000913/4541030‑4541303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑