Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	951,191	(A)6→5	intergenic (‑111/+81)	pflA ← / ← pflB	pyruvate formate‑lyase activating enzyme/pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	951,186	0	A	.	100.0%	254.5 / NA	60	intergenic (‑106/+86)	pflA/pflB	pyruvate formate‑lyase activating enzyme/pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (29/31);  total (29/31)

TTCAAAGGAGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTT  >  NC_000913/951048‑951321                                                                                                                                           |                                                                                                                                        ttCAAAGGAGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACaa                                                                                                                                        >  2:868162/1‑140 (MQ=255)  tCAAAGGAGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACaaa                                                                                                                                       >  2:537865/1‑140 (MQ=255)      aGGAGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACaaaaagg                                                                                                                                   >  2:451021/1‑138 (MQ=255)         aGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGctc                                                                                                                               >  1:608297/1‑140 (MQ=255)         aGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGctc                                                                                                                               >  1:345457/1‑140 (MQ=255)          gTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGctct                                                                                                                              <  1:185434/140‑1 (MQ=255)           tGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCtctc                                                                                                                             >  1:211081/1‑140 (MQ=255)           tGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCtctc                                                                                                                             <  2:231781/140‑1 (MQ=255)                    gcAATAGCTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTGCGTCGAGTCTGTTTTGGCAGTCGCCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTGGCGGTaaaa                                                                                                                    <  2:381533/139‑1 (MQ=255)                      aaTAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTaaaaaa                                                                                                                  <  2:345457/140‑1 (MQ=255)                              aCATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCAc                                                                                                          >  2:809029/1‑140 (MQ=255)                              aCATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCAc                                                                                                          >  2:723156/1‑140 (MQ=255)                                  tGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTc                                                                                                      >  2:1009297/1‑140 (MQ=255)                                  tGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTc                                                                                                      >  1:677658/1‑140 (MQ=255)                                    cGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGt                                                                                                    >  1:764918/1‑140 (MQ=255)                                    cGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGt                                                                                                    <  1:868162/140‑1 (MQ=255)                                    cGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGt                                                                                                    <  1:451021/140‑1 (MQ=255)                                      gtgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGt                                                                                                    <  2:129525/138‑1 (MQ=255)                                      gtgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGt                                                                                                    >  1:129525/1‑138 (MQ=255)                                      gtgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTgg                                                                                                  <  2:764918/140‑1 (MQ=255)                                      gtgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTgg                                                                                                  <  1:1009297/140‑1 (MQ=255)                                      gtgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTgg                                                                                                  <  1:809029/140‑1 (MQ=255)                                       tgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGa                                                                                                 <  1:537865/140‑1 (MQ=255)                                           tctcCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCt                                                                                             <  1:1050619/140‑1 (MQ=255)                                                  aTGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGt                                                                                      <  2:968468/140‑1 (MQ=255)                                                   tgtgGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTa                                                                                     <  1:423552/140‑1 (MQ=255)                                                   tgtgGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTa                                                                                     <  2:1131722/140‑1 (MQ=255)                                                      ggCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTgg                                                                                                  >  1:1170158/1‑124 (MQ=255)                                                      ggCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTgg                                                                                                  <  2:1170158/124‑1 (MQ=255)                                                       gCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGcc                                                                                              >  2:1177535/1‑127 (MQ=255)                                                       gCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGcc                                                                                              <  1:1177535/127‑1 (MQ=255)                                                             cTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTAc                                                                           <  1:723156/140‑1 (MQ=255)                                                               gAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTg                                                                         <  2:393207/140‑1 (MQ=255)                                                                 ggCCGTGTTGGTGCGCAGCTCGAAAGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTa                                                                       >  2:710829/1‑140 (MQ=255)                                                                      tgtTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAtt                                                                  >  2:1131949/1‑140 (MQ=255)                                                                      tgtTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAtt                                                                  >  1:534652/1‑140 (MQ=255)                                                                           gTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGt                                                             <  1:1131949/140‑1 (MQ=255)                                                                            tGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTc                                                            >  2:505027/1‑140 (MQ=255)                                                                            tGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTc                                                            >  2:663569/1‑140 (MQ=255)                                                                            tGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTc                                                            >  2:306403/1‑140 (MQ=255)                                                                                  gCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCt                                                      <  1:515550/140‑1 (MQ=255)                                                                                  gCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCt                                                      >  2:876888/1‑140 (MQ=255)                                                                                   cTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa                                                     <  1:876888/140‑1 (MQ=255)                                                                                        aagCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTAc                                                <  1:710829/138‑1 (MQ=255)                                                                                         ggCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACa                                               <  2:396098/140‑1 (MQ=255)                                                                                                    gTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa                                                     >  2:325927/1‑123 (MQ=255)                                                                                                    gTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTa                                                     <  1:325927/123‑1 (MQ=255)                                                                                                    gTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTg                                    <  1:306403/140‑1 (MQ=255)                                                                                                    gTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTg                                    >  2:143222/1‑140 (MQ=255)                                                                                                    gTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTAGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTg                                    >  2:1071823/1‑140 (MQ=255)                                                                                                            tGGCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACg                            <  1:745332/140‑1 (MQ=255)                                                                                                             ggCAGTCACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTGGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGa                           >  1:293118/1‑140 (MQ=255)                                                                                                                   cACCTTAAAGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGtaata                     >  2:351877/1‑140 (MQ=255)                                                                                                                         aaaGTATAGATAGCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTcc               <  2:608297/140‑1 (MQ=255)                                                                                                                              atagatagCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctg          <  2:534652/140‑1 (MQ=255)                                                                                                                                agatagCTGAC‑AAAAAGGCTCTGGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctgct        <  2:293118/140‑1 (MQ=255)                                                                                                                                agatagCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctgct        >  2:706752/1‑140 (MQ=255)                                                                                                                                agatagCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctgct        <  1:706752/140‑1 (MQ=255)                                                                                                                                agatagCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctgct        >  1:801968/1‑140 (MQ=255)                                                                                                                                  atagCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGt      <  1:351877/140‑1 (MQ=255)                                                                                                                                  atagCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGt      >  1:75201/1‑140 (MQ=255)                                                                                                                                    agCTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTc    >  2:722780/1‑140 (MQ=255)                                                                                                                                      cTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt  >  2:428676/1‑140 (MQ=255)                                                                                                                                      cTGAC‑AAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt  >  1:672868/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        TTCAAAGGAGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTT  >  NC_000913/951048‑951321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑