Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,384,133	(A)5→4	coding (17/1398 nt)	ycjX →	DUF463 domain‑containing protein YcjX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,384,129	0	A	.	100.0%	206.6 / NA	49	coding (13/1398 nt)	ycjX	DUF463 domain‑containing protein YcjX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (29/20);  total (29/20)

CACATCTTATGGAGTTCGCCGGTCGATGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGG  >  NC_000913/1383994‑1384262                                                                                                                                        |                                                                                                                                      cacaTCTTATGGAGTTCGCCGGTCGATGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTaaaat                                                                                                                                   >  2:266078/1‑139 (MQ=255)      cTTATGGAGTTCGCCGGTCGATGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACt                                                                                                                             <  1:755401/140‑1 (MQ=255)                tCGCCGGTCGATGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTg                                                                                                                   >  2:360580/1‑140 (MQ=255)                 cGCCGGTCGATGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTaaaatgaa                                                                                                                                >  2:517680/1‑123 (MQ=255)                 cGCCGGTCGATGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTaaaatgaa                                                                                                                                <  1:517680/127‑5 (MQ=255)                 cGCCGGTCGATGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATgcgc                                                                                                                     <  2:948683/137‑1 (MQ=255)                 cGCCGGTCGATGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATgcgc                                                                                                                     >  1:948683/1‑137 (MQ=255)                           tGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCgggg                                                                                                        <  2:512305/140‑1 (MQ=255)                              caacaCGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGgtgt                                                                                                     <  2:1211454/140‑1 (MQ=255)                              caacaCGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGgtgt                                                                                                     <  1:360580/140‑1 (MQ=255)                                   cGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCgaca                                                                                                <  1:1189098/140‑1 (MQ=255)                                                    aTGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCgacaga                                                                                              <  1:904994/125‑1 (MQ=255)                                                    aTGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCgacaga                                                                                              >  2:904994/1‑125 (MQ=255)                                                    aTGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCt                                                                               >  2:70363/1‑140 (MQ=255)                                                     tggcctggccCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTg                                                                              >  2:857849/1‑140 (MQ=255)                                                           ggccCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCg                                                                                 >  2:788108/1‑131 (MQ=255)                                                           ggccCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCg                                                                                 <  1:788108/131‑1 (MQ=255)                                                             cccccACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGa                                                                      >  1:824228/1‑140 (MQ=255)                                                              ccccACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGAc                                                                     <  2:106067/140‑1 (MQ=255)                                                                          atCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCg                                                         <  2:824228/140‑1 (MQ=255)                                                                          atCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCg                                                         >  1:818990/1‑140 (MQ=255)                                                                           tCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCgg                                                        <  1:857849/140‑1 (MQ=255)                                                                             cTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCa                                                      >  2:41656/1‑140 (MQ=255)                                                                                aCGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTTACCGGACTTAGCCGCAGCGGCaaaa                                                   >  1:812655/1‑140 (MQ=255)                                                                                aCGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGc                                                             >  2:1078319/1‑130 (MQ=255)                                                                                aCGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGc                                                             <  1:1078319/130‑1 (MQ=255)                                                                                aCGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCaaaa                                                   >  1:1161775/1‑140 (MQ=255)                                                                                aCGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCaaaa                                                   >  1:99831/1‑140 (MQ=255)                                                                                aCGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCaaaa                                                   >  1:819991/1‑140 (MQ=255)                                                                                aCGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCaaaa                                                   <  2:1161775/140‑1 (MQ=255)                                                                                  gAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACa                                                 >  1:888491/1‑140 (MQ=255)                                                                                       aTTTTTTTTCTGGTCTTCAGAGCAGCACTGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGtt                                            >  2:816581/1‑140 (MQ=255)                                                                                                cTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACcgc                                   <  2:943925/140‑1 (MQ=255)                                                                                                cTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGCGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACcgc                                   <  1:307010/140‑1 (MQ=255)                                                                                                             cagcaCAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAg                      <  2:818990/140‑1 (MQ=255)                                                                                                             cagcaCAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAg                      <  2:302041/140‑1 (MQ=255)                                                                                                             cagcaCAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAg                      >  2:531653/1‑140 (MQ=255)                                                                                                               gcaCAGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGtt                    >  2:169779/1‑140 (MQ=255)                                                                                                                cacaGGACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTg                   >  2:106687/1‑140 (MQ=255)                                                                                                                     gACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAAt                         <  1:216325/129‑1 (MQ=255)                                                                                                                     gACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAAt                         >  2:216325/1‑129 (MQ=255)                                                                                                                     gACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTaa              >  1:193218/1‑140 (MQ=255)                                                                                                                     gACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTaa              >  2:1056932/1‑140 (MQ=255)                                                                                                                     gACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTaa              >  2:955126/1‑140 (MQ=255)                                                                                                                     gACAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTaa              >  2:864958/1‑140 (MQ=255)                                                                                                                      aCAGCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAat             >  1:423974/1‑140 (MQ=255)                                                                                                                         gCAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATAtt          <  1:70363/140‑1 (MQ=255)                                                                                                                          cAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTc         >  2:640336/1‑140 (MQ=255)                                                                                                                          cAATGAAGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTc         >  1:543345/1‑140 (MQ=255)                                                                                                                               aaGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGcc    >  1:504172/1‑140 (MQ=255)                                                                                                                               aaGCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGcc    >  2:211965/1‑140 (MQ=255)                                                                                                                                 gCGACTT‑AAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCgg  <  2:99831/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      CACATCTTATGGAGTTCGCCGGTCGATGACAACACGGGCAGTTGATAATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGG  >  NC_000913/1383994‑1384262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑