Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,094	(G)8→9	noncoding (72/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,086	1	.	G	93.9%	162.5 / 3.2	49	noncoding (80/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (20/26);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGAC  >  NC_000913/4605948‑4606217                                                                                                                                             |                                                                                                                                    cacaAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGATTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑g                                                                                                                                    <  2:236892/140‑1 (MQ=255)    aaaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑gggg                                                                                                                                 <  2:1180125/140‑1 (MQ=255)     aaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑ggggg                                                                                                                                <  2:933128/140‑1 (MQ=255)       cAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑ggggggg                                                                                                                              <  1:168288/140‑1 (MQ=255)        aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑gggggggg                                                                                                                             >  2:1215948/1‑140 (MQ=255)          ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑ggggggggga                                                                                                                           >  1:573365/1‑138 (MQ=255)             cGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑gggggggggactt                                                                                                                        <  1:164753/140‑6 (MQ=255)                  ttACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAAcc                                                                                                                    >  1:771011/1‑140 (MQ=255)                    aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAAcccc                                                                                                                  <  1:751545/140‑1 (MQ=255)                    aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAAccca                                                                                                                  <  1:1215948/140‑2 (MQ=255)                      aTTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGTATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCa                                                                                                                <  1:1088956/140‑1 (MQ=255)                      aTTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCa                                                                                                                <  2:389171/140‑1 (MQ=255)                      aTTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCa                                                                                                                <  1:972553/140‑1 (MQ=255)                            cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGt                                                                                                         >  2:383025/1‑140 (MQ=255)                            cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGt                                                                                                         <  1:817919/140‑1 (MQ=255)                              aCCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                        <  1:1022804/140‑1 (MQ=255)                              aCCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTa                                                                                                        <  1:585690/140‑1 (MQ=255)                                cATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                      <  2:771011/140‑1 (MQ=255)                                cATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                      <  2:559456/140‑1 (MQ=255)                                    gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGaca                                                                                                  >  1:466220/1‑140 (MQ=255)                                      gCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                <  2:640964/140‑1 (MQ=255)                                        cTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                              <  2:466220/140‑1 (MQ=255)                                        cTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                              <  1:1096028/140‑1 (MQ=255)                                           gCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                                           >  2:1051728/1‑140 (MQ=255)                                           gCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                                           >  1:779066/1‑140 (MQ=255)                                            cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                          >  1:736305/1‑140 (MQ=255)                                                       ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                               <  2:1214851/140‑1 (MQ=255)                                                         catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                          >  2:380849/1‑127 (MQ=255)                                                         catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                          <  1:380849/127‑1 (MQ=255)                                                         catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                             >  1:769088/1‑140 (MQ=255)                                                             atGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                         >  2:575429/1‑140 (MQ=255)                                                                  gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                    >  2:864787/1‑140 (MQ=255)                                                                     gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                 <  2:469259/140‑1 (MQ=255)                                                                       tAATGAATTTGAATCAGGGGTGAAAAATCAAGGAAGAAACAAGAAAGGGAGTAAAGATAATTGGGGC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                               <  2:1192734/140‑1 (MQ=25)                                                                       tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                               <  2:1109783/140‑1 (MQ=255)                                                                        aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                               >  2:115861/1‑139 (MQ=255)                                                                        aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                               <  1:115861/139‑1 (MQ=255)                                                                         aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                             <  2:666825/140‑1 (MQ=255)                                                                         aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                             <  2:736305/140‑1 (MQ=255)                                                                               ttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                       <  1:260391/140‑1 (MQ=255)                                                                                 gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                     <  1:1051728/140‑1 (MQ=255)                                                                                 gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGAAACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTCGcc                                                     >  1:434309/1‑139 (MQ=255)                                                                                  aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAGGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                    <  2:315420/140‑1 (MQ=255)                                                                                  aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                    <  1:613105/140‑3 (MQ=255)                                                                                      aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTc                                                >  2:1077834/1‑140 (MQ=255)                                                                                               aaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                    >  2:337419/1‑127 (MQ=255)                                                                                               aaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                    <  1:337419/127‑1 (MQ=255)                                                                                                  tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGGCTTGAACCCCCACGTCCGCAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACCGTCATATTACttttt                                    >  2:340639/1‑140 (MQ=18)                                                                                                  tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCATCTTACttttt                                    >  2:778286/1‑140 (MQ=255)                                                                                                  tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACttttt                                    >  2:102808/1‑140 (MQ=255)                                                                                                           aaaCAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCg                           >  2:671680/1‑140 (MQ=255)                                                                                                                  aaaGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                    <  1:864787/140‑1 (MQ=255)                                                                                                                  aaaGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCCCGtt                    <  1:671680/140‑1 (MQ=255)                                                                                                                                tAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg       >  1:752072/1‑139 (MQ=255)                                                                                                                                tAATTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg       <  2:752072/139‑1 (MQ=255)                                                                                                                                 aaTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggggg     >  1:1156094/1‑140 (MQ=255)                                                                                                                                    tGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGAc  >  1:707797/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                    CACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGAC  >  NC_000913/4605948‑4606217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑