Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	321,292	(T)7→8	intergenic (+211/‑316)	rclR → / → ykgE	DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	321,285	1	.	T	100.0%	208.3 / NA	58	intergenic (+204/‑323)	rclR/ykgE	DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (25/33);  total (25/33)

TTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGA‑TTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTG  >  NC_000913/321146‑321417                                                                                                                                             |                                                                                                                                     tttctatttcCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGa                                                                                                                                       >  2:1200965/1‑140 (MQ=255)  ttctatttcCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGA‑t                                                                                                                                     >  1:549584/1‑140 (MQ=255)    ctatttcCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGA‑ttt                                                                                                                                   >  1:1023037/1‑140 (MQ=255)          ccAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGA‑ttttttttg                                                                                                                             <  2:1022559/140‑3 (MQ=255)            aaCGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGA‑ttttttttgta                                                                                                                           >  2:400597/1‑136 (MQ=255)            aaCGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGA‑ttttttttgta                                                                                                                           >  1:349101/1‑136 (MQ=255)               ggtggtTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTacac                                                                                                                         <  1:989953/140‑1 (MQ=255)               ggtggtTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTacac                                                                                                                         <  1:370318/140‑1 (MQ=255)                  ggtTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCAt                                                                                                                      <  2:350193/140‑1 (MQ=255)                   gtTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTacac                                                                                                                         <  1:783777/136‑1 (MQ=255)                   gtTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTacac                                                                                                                         >  2:783777/1‑136 (MQ=255)                    tttGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCa                                                                                                                    >  2:821438/1‑140 (MQ=255)                    tttGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCa                                                                                                                    <  1:1200965/140‑1 (MQ=255)                    tttGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCa                                                                                                                    <  2:549584/140‑1 (MQ=255)                       gggCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAgtg                                                                                                                 <  1:1183738/140‑1 (MQ=255)                       gggCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAgtg                                                                                                                 <  1:662544/140‑1 (MQ=255)                         gCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCAt                                                                                                                      >  1:420599/1‑133 (MQ=255)                         gCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCAt                                                                                                                      <  2:420599/133‑1 (MQ=255)                             tatataTTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATAtc                                                                                                           >  2:903799/1‑140 (MQ=255)                             tatataTTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATAtc                                                                                                           >  2:741493/1‑140 (MQ=255)                                   ttttttCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATa                                                                                                     <  1:614630/140‑1 (MQ=255)                                       ttCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAAccc                                                                                                 >  2:560284/1‑140 (MQ=255)                                        tCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAAcccc                                                                                                <  1:587819/140‑1 (MQ=255)                                             tGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAAt                                                                                           >  2:1113928/1‑140 (MQ=255)                                                aCTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAAcc                                                                                                  <  1:337748/130‑1 (MQ=255)                                                aCTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAAcc                                                                                                  >  2:337748/1‑130 (MQ=255)                                                aCTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAg                                                                                        <  2:1037696/140‑1 (MQ=255)                                                  tctAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGa                                                                                             >  2:490920/1‑133 (MQ=255)                                                  tctAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGa                                                                                             <  1:490920/133‑1 (MQ=255)                                                     aGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGct                                                                                   <  2:339943/140‑1 (MQ=255)                                                     aGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGct                                                                                   <  2:1177184/140‑1 (MQ=255)                                                     aGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGct                                                                                   <  1:633585/140‑1 (MQ=255)                                                     aGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGct                                                                                   <  1:560284/140‑1 (MQ=255)                                                     aGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGct                                                                                   <  2:1023037/140‑1 (MQ=255)                                                      gCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGctc                                                                                  <  1:501188/140‑1 (MQ=255)                                                         cAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGa                                                                               <  1:987389/140‑1 (MQ=255)                                                          aGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGaa                                                                              <  1:903799/140‑1 (MQ=255)                                                          aGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGaa                                                                              <  1:745841/140‑1 (MQ=255)                                                           gTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAAt                                                                             <  1:821438/140‑1 (MQ=255)                                                            tttttAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATa                                                                            >  2:740604/1‑140 (MQ=255)                                                              tttAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAgg                                                                          <  1:741493/140‑1 (MQ=255)                                                               ttAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGt                                                                         <  1:740604/140‑1 (MQ=255)                                                                                    aTTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCAttttt                                                    <  1:1113928/140‑1 (MQ=255)                                                                                         ttCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATg                                               >  1:972398/1‑140 (MQ=255)                                                                                             aTTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGAtt                                           >  1:588354/1‑140 (MQ=255)                                                                                                        tCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCaa                                >  2:800054/1‑140 (MQ=255)                                                                                                           cGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGGCATTTTTAAATGGATTTCTTATGCCaagaa                             >  2:753714/1‑140 (MQ=255)                                                                                                            gACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAAt                            <  1:800054/140‑1 (MQ=255)                                                                                                             aCTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATg                           >  2:249796/1‑140 (MQ=255)                                                                                                              cTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGa                          >  2:1213941/1‑140 (MQ=255)                                                                                                                 ttACTTGATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGAtcg                       >  1:871929/1‑140 (MQ=255)                                                                                                                       gATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCaaaaa                             <  2:1211021/128‑1 (MQ=255)                                                                                                                       gATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCaaaaa                             >  1:1211021/1‑128 (MQ=255)                                                                                                                       gATACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGAtt                 >  1:574039/1‑140 (MQ=255)                                                                                                                         tACGTGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTc               >  1:149799/1‑140 (MQ=255)                                                                                                                             tGATTATTGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTcccaa           <  1:753714/140‑3 (MQ=255)                                                                                                                               attattGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATa         <  2:574039/140‑1 (MQ=255)                                                                                                                               attattGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATa         <  2:349101/140‑1 (MQ=255)                                                                                                                                 tattGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGAttt                <  1:335122/131‑1 (MQ=255)                                                                                                                                 tattGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGAttt                >  2:335122/1‑131 (MQ=255)                                                                                                                                  attGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATaaaa      >  2:1216661/1‑140 (MQ=255)                                                                                                                                   ttGTCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAAt     >  2:635792/1‑140 (MQ=255)                                                                                                                                      tCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAAtgtg  >  2:1147436/1‑140 (MQ=255)                                                                                                                                      tCGGTGATTTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAAtgtg  >  1:202945/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                     TTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGA‑TTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTG  >  NC_000913/321146‑321417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑