Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	834,668	(A)6→7	coding (1599/2151 nt)	dinG →	ATP‑dependent DNA helicase DinG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	834,662	1	.	A	100.0%	181.7 / NA	51	S531R (AGC→AGA)	dinG	ATP‑dependent DNA helicase DinG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (26/25);  total (26/25)

TGTGGCGCTGGATTCCCCCTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGC‑AAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGCCGCGTTACCGTTTAGTTGAACTG  >  NC_000913/834524‑834794                                                                                                                                            |                                                                                                                                     tgtgGCGCTGGATTCCCCCTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGC‑a                                                                                                                                     >  2:1088767/1‑140 (MQ=255)         tGGATTCCCCCTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGC‑aaaaaaaca                                                                                                                             <  2:1053128/140‑4 (MQ=255)           gATTCCCCCTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTGCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGC‑aaaa                                                                                                                                  <  2:870476/133‑1 (MQ=255)           gATTCCCCCTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGC‑aaaa                                                                                                                                  >  1:870476/1‑133 (MQ=255)               cccccTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCgg                                                                                                                        <  2:360462/140‑1 (MQ=255)               cccccTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCgg                                                                                                                        <  1:1088767/140‑1 (MQ=255)               cccccTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCgg                                                                                                                        <  1:1172465/140‑1 (MQ=255)                 cccTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTa                                                                                                                      <  1:332136/140‑1 (MQ=255)                     ttAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCgg                                                                                                                        <  2:779394/134‑1 (MQ=255)                     ttAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCgg                                                                                                                        >  1:779394/1‑134 (MQ=255)                     ttAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGtt                                                                                                                  >  1:691004/1‑140 (MQ=255)                          cACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTAc                                                                                                             >  1:587611/1‑140 (MQ=255)                            cTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTg                                                                                                           >  2:184135/1‑140 (MQ=255)                             tGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCgg                                                                                                                        <  2:767603/126‑1 (MQ=255)                             tGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCgg                                                                                                                        >  1:767603/1‑126 (MQ=255)                                  aCAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTa                                                                                                                      <  1:7997/123‑1 (MQ=255)                                  aCAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTa                                                                                                                      >  2:7997/1‑123 (MQ=255)                                      ggCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAg                                                                                                   >  1:547078/1‑138 (MQ=255)                                      ggCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAg                                                                                                   <  2:547078/138‑1 (MQ=255)                                      ggCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCg                                                                                                 >  1:1116380/1‑140 (MQ=255)                                       gCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCgg                                                                                                <  1:178352/140‑1 (MQ=255)                                       gCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCgg                                                                                                <  1:184135/140‑1 (MQ=255)                                                ttATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGAt                                                                                       >  2:1044667/1‑140 (MQ=255)                                                 tATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATg                                                                                      <  2:1150457/140‑1 (MQ=255)                                                     ccccGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAgc                                                                                  <  2:691004/140‑1 (MQ=255)                                                        cGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCt                                                                               >  2:1234459/1‑140 (MQ=255)                                                        cGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCt                                                                               >  2:1059657/1‑140 (MQ=255)                                                           aTGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTtc                                                                            <  2:1116380/140‑1 (MQ=255)                                                             gcgcGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTtctc                                                                          <  1:1044667/140‑1 (MQ=255)                                                              cgcgTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAgc                                                                                  <  1:920419/131‑1 (MQ=255)                                                              cgcgTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAgc                                                                                  >  2:920419/1‑131 (MQ=255)                                                                cgTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAgc                                                                                  >  1:111223/1‑129 (MQ=255)                                                                cgTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAgc                                                                                  <  2:111223/129‑1 (MQ=255)                                                                        cTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGAc                                                               <  1:1217561/140‑1 (MQ=255)                                                                                gACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTAc                                                       >  2:1147817/1‑140 (MQ=255)                                                                                     cGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGt                                                     >  1:85448/1‑137 (MQ=255)                                                                                     cGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGt                                                     <  2:85448/137‑1 (MQ=255)                                                                                     cGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTg                                                  >  2:391522/1‑140 (MQ=255)                                                                                     cGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTg                                                  >  1:722530/1‑140 (MQ=255)                                                                                     cGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTg                                                  >  1:1204896/1‑140 (MQ=255)                                                                                           gcagcaTATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTg                                            <  1:1234459/140‑1 (MQ=255)                                                                                           gcagcaTATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTg                                            <  2:722530/140‑1 (MQ=255)                                                                                               caTATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTgg                                        >  1:916820/1‑140 (MQ=255)                                                                                               caTATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTgg                                        >  1:1088243/1‑140 (MQ=255)                                                                                                    tGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAg                                   >  2:415781/1‑140 (MQ=255)                                                                                                           aTGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATc                            >  2:274717/1‑140 (MQ=255)                                                                                                               cggcCTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGcc                        >  1:655256/1‑140 (MQ=255)                                                                                                                   cTTTTTCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGCCGCGt                    <  1:1059657/140‑1 (MQ=255)                                                                                                                    tttttCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGCCGCGtt                   <  2:916820/140‑1 (MQ=255)                                                                                                                        tCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGCCGCGTTACCg               <  2:655256/140‑1 (MQ=255)                                                                                                                        tCCGTAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGCCGCGTTACCg               <  2:587611/140‑1 (MQ=255)                                                                                                                            tAAGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGCCGCGTTACCGTTTa           >  2:924729/1‑140 (MQ=255)                                                                                                                             aaGCAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTGGACTATGTGACGGATTTACGTCTGATGTTGATGGTTCAGGGCGATCAGCCGCGTTACCGTTTAg          >  2:983139/1‑140 (MQ=255)                                                                                                                                cAGGTGGAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGCCGCGTTACCGTTTAGTTg       >  1:302808/1‑140 (MQ=255)                                                                                                                                     ggAGAGCAAAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGCCGCGTTACCGTTTAGTTGAACTg  <  1:1147817/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     TGTGGCGCTGGATTCCCCCTTTAACCACTGCGAACAGGGCAAAATTGTTATTCCCCGGATGCGCGTTGAGCCTTCCATCGACAACGAAGAGCAGCATATTGCCGAAATGGCGGCCTTTTTCCGTAAGCAGGTGGAGAGC‑AAAAAACATCTCGGTATGTTGGTACTGTTTGCCAGCGGACGGGCGATGCAGCGCTTTCTCGACTATGTGACGGATTTACGTCTGATGTTGCTGGTTCAGGGCGATCAGCCGCGTTACCGTTTAGTTGAACTG  >  NC_000913/834524‑834794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑