Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	86,365	(G)5→6	coding (736/1725 nt)	ilvI →	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	86,360	1	.	G	100.0%	209.5 / NA	60	L244R (CTG→CGG)	ilvI	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (31/29);  total (31/29)

TGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCT‑GGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACG  >  NC_000913/86223‑86493                                                                                                                                           |                                                                                                                                      tGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCT‑gg                                                                                                                                     <  1:1096223/140‑1 (MQ=255)  gCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCT‑ggg                                                                                                                                    <  2:62853/140‑1 (MQ=255)   ctctGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCT‑gggg                                                                                                                                   >  2:51850/1‑140 (MQ=255)   ctctGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGAGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCT‑gggg                                                                                                                                   >  1:325203/1‑140 (MQ=255)        cAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCT‑ggggggcgt                                                                                                                              >  2:330657/1‑136 (MQ=255)        cAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCAGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCT‑ggggggcgt                                                                                                                              >  1:698623/1‑136 (MQ=255)              cTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGTCATCAGCAGTTGAAAGAAACGGGGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCgg                                                                                                                         >  2:103580/1‑140 (MQ=255)                ggTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGc                                                                                                                        <  2:106108/139‑1 (MQ=255)                ggTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGc                                                                                                                        >  1:106108/1‑139 (MQ=255)                  tAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAAc                                                                                                                     >  1:803911/1‑140 (MQ=255)                  tAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAAc                                                                                                                     <  1:739986/140‑1 (MQ=255)                     cGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCa                                                                                                                  <  2:900368/140‑1 (MQ=255)                     cGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCa                                                                                                                  <  1:1154428/140‑1 (MQ=255)                      ggCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTTTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCa                                                                                                                  >  2:1149199/1‑139 (MQ=255)                      ggCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCa                                                                                                                  <  1:1149199/139‑1 (MQ=255)                      ggCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCAt                                                                                                                 >  1:1190098/1‑140 (MQ=255)                       gCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATc                                                                                                                >  1:887946/1‑140 (MQ=255)                       gCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATc                                                                                                                >  1:196378/1‑140 (MQ=255)                       gCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAAAGGTGGGGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATc                                                                                                                >  1:792930/1‑140 (MQ=255)                          aaaaaaCCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTc                                                                                                             <  1:52551/140‑1 (MQ=255)                               aCCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCa                                                                                                        <  1:417175/140‑1 (MQ=255)                                  ggTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTg                                                                                                     >  1:1177823/1‑140 (MQ=255)                                    ttGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTggg                                                                                                   <  2:196378/140‑1 (MQ=255)                                         tACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGc                                                                                              >  1:783832/1‑140 (MQ=255)                                         tACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGc                                                                                              <  1:275489/140‑1 (MQ=255)                                              aGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGa                                                                                         <  1:330657/140‑1 (MQ=255)                                                gCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGGGTTTCCGGCAACCCATCGTCAGGCACTGGGCATGCTGGGAAt                                                                                       >  1:910565/1‑140 (MQ=255)                                                gCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAAt                                                                                       >  1:84735/1‑140 (MQ=255)                                                gCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGGTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGaaa                                                                                       >  2:550338/1‑139 (MQ=255)                                                 cGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATg                                                                                      <  1:1070990/140‑1 (MQ=255)                                                 cGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATg                                                                                      >  1:905294/1‑140 (MQ=255)                                                   gTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCa                                                                                    <  2:515668/140‑1 (MQ=255)                                                   gTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCa                                                                                    <  1:611692/140‑1 (MQ=255)                                                   gTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCa                                                                                    >  2:605/1‑140 (MQ=255)                                                    tGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCAc                                                                                   <  2:905294/140‑1 (MQ=255)                                                     ggggCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGaa                                                                                        >  1:248263/1‑134 (MQ=255)                                                     ggggCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGaa                                                                                        <  2:248263/134‑1 (MQ=255)                                                     ggggCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCa                                                                                    <  2:30963/138‑1 (MQ=255)                                                     ggggCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCa                                                                                    >  1:30963/1‑138 (MQ=255)                                                          aaTCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTAcc                                                                             >  1:1133001/1‑140 (MQ=255)                                                           aTCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCt                                                                            <  2:887946/140‑1 (MQ=255)                                                            tCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTa                                                                           >  1:791198/1‑140 (MQ=255)                                                              aCGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACg                                                                         <  2:1190098/140‑1 (MQ=255)                                                                      cTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAat                                                                 >  2:939497/1‑140 (MQ=255)                                                                      cTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAat                                                                 >  1:8082/1‑140 (MQ=255)                                                                          cATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGa                                                             <  1:460454/140‑1 (MQ=255)                                                                           aTCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGAc                                                            <  1:51850/140‑1 (MQ=255)                                                                            tCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACg                                                           <  2:8082/140‑1 (MQ=255)                                                                               gcagTTGAAAGAGACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATg                                                        <  1:181167/140‑1 (MQ=255)                                                                                   ttGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATa                                                    >  2:1205980/1‑140 (MQ=255)                                                                                   ttGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATa                                                    >  1:1186045/1‑140 (MQ=255)                                                                                     gaaagaaaCGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAAc                                                  <  1:1093813/140‑1 (MQ=255)                                                                                         gaaaCGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCgg                                              <  1:939497/140‑1 (MQ=255)                                                                                             cGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGAtgt                                          >  1:888515/1‑140 (MQ=255)                                                                                                        ttGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCg                               <  2:325203/140‑1 (MQ=255)                                                                                                        ttGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCg                               >  1:316997/1‑140 (MQ=255)                                                                                                          gAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTc                             >  1:1175757/1‑140 (MQ=255)                                                                                                           aaTCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCg                            <  2:698623/140‑1 (MQ=255)                                                                                                              cTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCgggg                         <  2:1177823/140‑1 (MQ=255)                                                                                                                      tgttTGCTCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGAttt                 >  1:1025860/1‑140 (MQ=255)                                                                                                                             tCATTGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGAcc          <  1:963307/140‑1 (MQ=255)                                                                                                                                ttGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGaa       >  1:458219/1‑140 (MQ=255)                                                                                                                                ttGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGaa       >  2:382284/1‑140 (MQ=255)                                                                                                                                ttGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGaa       >  1:945730/1‑140 (MQ=255)                                                                                                                                ttGATGGGGCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGaa       >  1:961835/1‑140 (MQ=255)                                                                                                                                     ggggCTGGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAacgacg  <  1:1205980/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      TGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCT‑GGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACG  >  NC_000913/86223‑86493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑