| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,000,875 | (C)5→6 | coding (531/2259 nt) | xdhA → | putative xanthine dehydrogenase molybdenum‑binding subunit XdhA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,000,870 | 1 | . | C | 95.7% | 158.1 / 0.1 | 46 | coding (526/2259 nt) | xdhA | putative xanthine dehydrogenase molybdenum‑binding subunit XdhA |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (21/23); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
GCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GA‑CCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGA > NC_000913/3000734‑3001003 | gCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GA‑ccc > 2:1196102/1‑140 (MQ=255) ccAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GA‑cccc > 1:579089/1‑140 (MQ=255) cAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GA‑ccccc < 2:288508/140‑1 (MQ=255) aaGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GA‑ccccccgt > 2:479352/1‑137 (MQ=255) aGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GA‑ccccccgtt < 2:579089/140‑5 (MQ=255) ggcgCTGGCAGAAGACGCTGCACGAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTAtt < 1:1152910/140‑1 (MQ=255) ggcgCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTAtt < 1:676669/140‑1 (MQ=255) ggcgCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTAtt < 2:35859/140‑1 (MQ=255) gcgcTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTc > 2:194142/1‑140 (MQ=255) cgcTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCa < 1:588921/140‑1 (MQ=255) gCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACAtt < 1:212025/140‑1 (MQ=255) cTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGa > 2:740575/1‑140 (MQ=255) cTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGa < 1:1056779/140‑1 (MQ=255) aTCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTaa > 2:253577/1‑140 (MQ=255) cGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTg > 2:430044/1‑140 (MQ=255) gTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGc < 1:430044/140‑1 (MQ=255) tGGCAATTTACTGAGACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg > 1:766075/1‑140 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGGC‑CCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGc > 2:925499/1‑139 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGGC‑CCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGc < 1:925499/139‑1 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg > 2:334864/1‑140 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg > 1:164436/1‑140 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg > 2:712574/1‑140 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg > 2:1041961/1‑140 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg > 2:238001/1‑140 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg > 2:128021/1‑140 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg < 2:514031/140‑1 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg > 2:510835/1‑140 (MQ=255) tGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGACACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCg < 2:140864/140‑1 (MQ=255) ggCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGt < 1:510835/140‑1 (MQ=255) aaTTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGtgga < 1:194142/140‑1 (MQ=255) aaCAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGa > 2:824545/1‑140 (MQ=255) aaGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATTACTcg < 1:334864/140‑1 (MQ=255) cGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAAt > 2:536930/1‑140 (MQ=255) gATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAAtt > 1:632993/1‑140 (MQ=255) tGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTAc > 1:641266/1‑140 (MQ=255) gACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATc < 1:128021/140‑1 (MQ=255) gACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATc < 2:632993/140‑1 (MQ=255) tAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTcc < 1:646604/140‑1 (MQ=255) ccAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCAcc < 1:1041961/140‑1 (MQ=255) ccgccgACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGt < 1:1196102/140‑1 (MQ=255) ccgACTACCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTcg < 1:253577/140‑1 (MQ=255) aCCAGGTACAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGt < 2:641266/140‑1 (MQ=255) cAGGGGCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTc > 2:229358/1‑140 (MQ=255) aGGGGCACTATCA‑TACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCa > 1:1207341/1‑140 (MQ=255) gggCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAgg > 2:306379/1‑140 (MQ=255) gggCACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAgg > 1:1096015/1‑140 (MQ=255) cACTATCA‑TACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGgcgc < 2:1207341/140‑1 (MQ=255) cACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGgcgc < 1:238001/140‑1 (MQ=255) cACTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGgcgc < 1:712574/140‑1 (MQ=255) cTATCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTg > 1:400922/1‑140 (MQ=255) aTCA‑GACCCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGa < 1:740575/140‑1 (MQ=255) | GCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCA‑GA‑CCCCCGTTATTCAACATTGTCACATGGAAAGCGTAACATCGCTGGCGTGGATGGAGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGA > NC_000913/3000734‑3001003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |