Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,156,878	(C)5→6	coding (256/828 nt)	dkgA →	methylglyoxal reductase DkgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,156,873	1	.	C	100.0%	295.1 / NA	82	R84P (CGC→CCC)	dkgA	methylglyoxal reductase DkgA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (41/41);  total (41/41)

AGAAGTGGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCG‑CCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCAAAAAG  >  NC_000913/3156736‑3157007                                                                                                                                           |                                                                                                                                       aGAAGTGGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCG‑cc                                                                                                                                      <  1:659496/140‑1 (MQ=255)       gggTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCG‑ccccccgc                                                                                                                                >  1:147517/1‑137 (MQ=255)           tATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAg                                                                                                                             <  1:1216037/140‑1 (MQ=255)           tATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAg                                                                                                                             <  2:693711/140‑1 (MQ=255)              cGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGccc                                                                                                                          >  1:115064/1‑140 (MQ=255)                     ttGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGa                                                                                                                   <  1:496780/140‑1 (MQ=255)                     ttGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGa                                                                                                                   <  1:550505/140‑1 (MQ=255)                      tGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCGGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGAc                                                                                                                  <  1:708225/140‑1 (MQ=255)                              ccgcGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGaa                                                                                                          >  1:875324/1‑140 (MQ=255)                               cgcgGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGaaa                                                                                                         >  1:204966/1‑140 (MQ=255)                               cgcgGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGaaa                                                                                                         >  1:144819/1‑140 (MQ=255)                                 cgGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGaaaaa                                                                                                       >  1:656676/1‑140 (MQ=255)                                 cgGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGaaaaa                                                                                                       >  1:745394/1‑140 (MQ=255)                                  ggCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGaaaaaa                                                                                                      >  1:332464/1‑140 (MQ=255)                                   gCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAAc                                                                                                     >  2:84131/1‑140 (MQ=255)                                     cTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTc                                                                                                   <  2:745394/140‑1 (MQ=255)                                        cAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                <  2:707314/140‑1 (MQ=255)                                        cAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                <  2:204966/140‑1 (MQ=255)                                        cAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAg                                                                                                <  2:675724/140‑1 (MQ=255)                                          aGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTTGAACGTTGTCCACAAGCGCCCCCCGCGACGGCCTGCTCGACAGCCTGCAAAATCGCCAGCt                                                                                              <  2:665073/140‑1 (MQ=255)                                             aCGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGa                                                                                           <  2:144819/140‑1 (MQ=255)                                              cGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGAt                                                                                          >  2:1010149/1‑140 (MQ=255)                                              cGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGAt                                                                                          >  1:852608/1‑140 (MQ=255)                                              cGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGAt                                                                                          >  1:1182877/1‑140 (MQ=255)                                                aagaagGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATta                                                                                        >  1:581477/1‑140 (MQ=255)                                                aagaagGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATta                                                                                        >  1:234870/1‑140 (MQ=255)                                                  gaagGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATtata                                                                                      >  2:1139819/1‑140 (MQ=255)                                                    agGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATc                                                                                    <  2:255524/140‑1 (MQ=255)                                                    agGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATc                                                                                    <  1:1038423/140‑1 (MQ=255)                                                    agGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATc                                                                                    <  1:1150198/140‑1 (MQ=255)                                                    agGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATc                                                                                    <  1:1010149/140‑1 (MQ=255)                                                      gtgtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGa                                                                                  >  2:6230/1‑140 (MQ=255)                                                       tgtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGAc                                                                                 >  1:551548/1‑140 (MQ=255)                                                        gtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGAcc                                                                                <  2:147517/140‑1 (MQ=255)                                                        gtCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGAcc                                                                                <  2:115064/140‑1 (MQ=255)                                                         tCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACct                                                                               <  2:875324/140‑1 (MQ=255)                                                         tCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACct                                                                               <  2:278485/140‑1 (MQ=255)                                                         tCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACct                                                                               <  1:1139819/140‑1 (MQ=255)                                                         tCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACct                                                                               <  2:1203917/140‑1 (MQ=255)                                                         tCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACct                                                                               <  1:135559/140‑1 (MQ=255)                                                           ggCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACctct                                                                             <  2:900505/140‑1 (MQ=255)                                                             cAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTAc                                                                           >  2:775922/1‑140 (MQ=255)                                                                      gAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCAc                                                                  >  2:327743/1‑140 (MQ=255)                                                                            tGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGccc                                                            <  2:215335/140‑1 (MQ=255)                                                                              ccTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGt                                                          <  2:551548/140‑1 (MQ=255)                                                                                 cAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTcc                                                       >  1:328574/1‑140 (MQ=255)                                                                                   gTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTcgcg                                                     <  2:1182877/140‑1 (MQ=255)                                                                                        cAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATc                                                <  2:332464/140‑1 (MQ=255)                                                                                        cAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATc                                                <  2:92071/140‑1 (MQ=255)                                                                                           agaagaACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGAc                                             <  1:84131/140‑1 (MQ=255)                                                                                            gaagaaCTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGAcc                                            >  1:1084584/1‑140 (MQ=255)                                                                                             aagaaCTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCa                                           <  1:702031/140‑1 (MQ=255)                                                                                             aagaaCTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCa                                           >  1:500242/1‑140 (MQ=255)                                                                                                     ttCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCg                                   >  1:486167/1‑140 (MQ=255)                                                                                                     ttCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCg                                   >  1:373179/1‑140 (MQ=255)                                                                                                     ttCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCg                                   >  1:852851/1‑140 (MQ=255)                                                                                                     ttCATCACCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCg                                   >  1:904009/1‑140 (MQ=255)                                                                                                       catcaCCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGaa                                 <  2:234870/140‑1 (MQ=255)                                                                                                        atcaCCACTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAg                                <  1:327743/140‑1 (MQ=255)                                                                                                          caccacTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCa                              >  1:319436/1‑140 (MQ=255)                                                                                                              acTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGATCATTATGTCGAAGCATGGa                          <  1:6230/140‑1 (MQ=255)                                                                                                               cTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCAtt                                         <  1:571256/124‑1 (MQ=255)                                                                                                               cTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCAtt                                         >  2:571256/1‑124 (MQ=255)                                                                                                               cTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGaa                         >  1:192931/1‑140 (MQ=255)                                                                                                               cTAAGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGACCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGaa                         >  1:909340/1‑140 (MQ=255)                                                                                                                  aGCTGTGGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAgg                      >  1:1195474/1‑140 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCTAAGCATGGAAAGGCATGa                 >  1:943034/1‑140 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGa                 >  2:944403/1‑140 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGa                 >  2:548124/1‑140 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGa                 >  1:37732/1‑140 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGa                 >  2:1163469/1‑140 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGa                 >  1:425960/1‑140 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGa                 >  1:886794/1‑140 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGa                 >  1:591381/1‑140 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATATCGAAGCAt                             <  1:226800/128‑1 (MQ=255)                                                                                                                       tgGAACGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATATCGAAGCAt                             >  2:226800/1‑128 (MQ=255)                                                                                                                          aaCGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCg              <  2:425960/140‑1 (MQ=255)                                                                                                                          aaCGACGACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCg              <  2:37732/140‑1 (MQ=255)                                                                                                                           acgacgACCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGa             <  2:852851/140‑1 (MQ=255)                                                                                                                            cgacgaCCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGaa            <  2:581477/140‑1 (MQ=255)                                                                                                                              acgacCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAAtt          >  2:771861/1‑140 (MQ=255)                                                                                                                                 acCACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCa       >  1:353588/1‑140 (MQ=255)                                                                                                                                  ccACAAGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTACTGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCaa      <  2:1084584/140‑1 (MQ=255)                                                                                                                                      aaGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCAAAAag  <  2:1195474/140‑1 (MQ=255)                                                                                                                                      aaGCGCCCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCAAAAag  <  2:656676/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       AGAAGTGGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTGGAACGACGACCACAAGCG‑CCCCCGCGAAGCCCTGCTCGACAGCCTGAAAAAACTCCAGCTTGATTATATCGACCTCTACTTAATGCACTGGCCCGTTCCCGCTATCGACCATTATGTCGAAGCATGGAAAGGCATGATCGAATTGCAAAAAG  >  NC_000913/3156736‑3157007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑