| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 35.5% | 41.8 / 16.3 | 31 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (1/19); new base G (11/0); total (12/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA > NC_000913/646619‑646886 | gTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑cccc < 1:468821/140‑1 (MQ=255) tGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑ccccc > 1:73034/1‑140 (MQ=255) gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCt < 2:366917/140‑1 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGTGGTGTGAATC‑tcccccc > 2:236120/1‑138 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCtt > 1:524837/1‑140 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑cccccct > 1:399285/1‑138 (MQ=255) ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCttt < 1:25018/140‑1 (MQ=255) gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAAG‑CCCCCCTTTTTTAATAAAGTTTgtgc > 1:518680/1‑140 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTc < 2:292114/140‑1 (MQ=255) aaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGttt < 2:975851/132‑1 (MQ=255) aaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTGCAATAATGttt > 1:975851/1‑132 (MQ=255) cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGATG‑GCCCCCTTTTTTTATAATTTTTTCGCCCcgccccg > 2:1194712/1‑136 (MQ=255) ggTAAGGATCAGTAGGTCAGCGCTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTTAATAAAATTTTCGCCTCCCGCCGtg > 2:873397/1‑139 (MQ=255) ggATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAg < 1:936295/140‑1 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAgg < 1:236120/140‑1 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTTAATAATGTTTTCGCCCCCCGCCGTAGCCAGCGCCgg > 2:964338/1‑140 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCTTTTTTCAATAATTTTTTCGCCTCCCGCTGTAGCCAGCGCAgg > 1:476621/1‑140 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCTGTAGCCAGCGCAga > 1:1026354/1‑139 (MQ=255) tAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCTTTTTTCCATAATGTTTTCGCCTCCCGCTGTAGCCCGCGCCGGc > 1:1105118/1‑140 (MQ=255) ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATG‑CCCCCCTTTTTTTATAATTTTTTCGCCTCCCGCCGTAGCCAGCGCCGGccc > 1:1163093/1‑140 (MQ=255) gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTTAATAATATTTTTGCCCCCCGCCGTAGCCCGCGCACGcccc > 2:331871/1‑140 (MQ=255) cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATT‑TCCCCCCTTTTTAAAAAAATTTTTGCCTCGCCCCGTCGGCAGCGCCGGccccccccc > 2:241406/1‑137 (MQ=255) cTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTTAATAATGTTTTTGCCTCCCGCTGTAGCCAGCGCCGGccccccccccc > 1:829564/1‑135 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 1:110547/140‑1 (MQ=255) gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCCCACGACCCCCTTTTTG‑AATTATTATTTCGCCTCCCGCTGTTGCCAGCGCGAGCCCCCCCCGCCCCCCCCCGATggca > 2:23136/1‑137 (MQ=255) aTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑GCCCCCTTTTTTAATAATGTTTTTGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCCCGCCACCCCGCGCGGCAACGt > 2:965918/1‑140 (MQ=255) cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCGCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGa < 2:907179/140‑1 (MQ=255) tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGAt < 1:873397/140‑1 (MQ=255) ccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCg < 2:1163093/140‑1 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc < 1:965918/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 2:518680/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg < 2:524837/140‑1 (MQ=255) ccGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTTAATAATGTTTTTGCCCCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAggg > 2:377809/1‑139 (MQ=255) ggAGATAATCGAGATCGGCGGGGGGTCGAAAG‑CCCCCCTTTTTTAAAAATTTTTTCGCCCCCCGCCGTAGGCCGCGCAGGCCCCCCCCCCCCCCCCCCCGTGCAACGTTGGGATCGCCGCTGATCGCCCTCAGTAggggg > 2:1111309/1‑137 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:146514/140‑1 (MQ=255) gaTCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc < 2:396140/140‑1 (MQ=255) gaTCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGACCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc < 2:1026354/140‑1 (MQ=255) gcggGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc < 1:1218960/140‑1 (MQ=255) gcggGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc < 2:476621/140‑1 (MQ=255) cggGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGcc < 1:96603/140‑1 (MQ=255) ttCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCa < 1:1221918/140‑1 (MQ=255) | GTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA > NC_000913/646619‑646886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |