Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,257	T→C	Q68R (CAG→CGG)	yaaA ←	peroxide stress resistance protein YaaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,257	0	T	C	100.0%	138.1 / NA	42	Q68R (CAG→CGG)	yaaA	peroxide stress resistance protein YaaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/24);  total (18/24)

GCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCA  >  NC_000913/6121‑6389                                                                                                                                          |                                                                                                                                     gCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGgccgcc                                                                                                                                   >  1:1034510/1‑140 (MQ=255) gCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGgccgcc                                                                                                                                   <  2:1154867/140‑1 (MQ=255) gCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGgccgcc                                                                                                                                   <  1:314714/140‑1 (MQ=255)  ccGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCa                                                                                                                                  <  2:78319/140‑1 (MQ=255)    ggAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGt                                                                                                                                >  1:684537/1‑140 (MQ=255)      aaaGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCa                                                                                                                              <  2:343907/140‑1 (MQ=255)      aaaGCATTCGCAAATGCTGTTGGGAAAAATCGAAATCGGCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGAAATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCa                                                                                                                              <  2:587837/140‑1 (MQ=255)        aGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATg                                                                                                                            <  2:684537/140‑1 (MQ=255)           aTTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGaaa                                                                                                                         >  1:955166/1‑140 (MQ=255)           aTTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAAACTGGCCGCCAGTCATGaaa                                                                                                                         >  1:608270/1‑140 (MQ=255)               gCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAagcga                                                                                                                     <  2:945908/140‑1 (MQ=255)                cAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAagcgag                                                                                                                    <  2:188410/140‑1 (MQ=255)                cAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAagcgag                                                                                                                    <  1:474253/140‑1 (MQ=255)                 aaaTGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAagcgagc                                                                                                                   <  2:10350/140‑1 (MQ=255)                 aaaTGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAagcgagc                                                                                                                   <  2:861979/140‑1 (MQ=255)                  aaTGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAagcgagcg                                                                                                                  <  1:20077/140‑1 (MQ=255)                  aaTGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAagcgagcg                                                                                                                  <  2:1162705/140‑1 (MQ=255)                      cTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGt                                                                                                              <  1:500261/140‑1 (MQ=255)                        gTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTg                                                                                                            <  2:833027/140‑1 (MQ=255)                                 aatcgaaatcgTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGcc                                                                                                   >  1:209480/1‑140 (MQ=255)                                                     aaGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCAt                                                                               >  2:58558/1‑140 (MQ=255)                                                     aaGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCAt                                                                               >  1:172057/1‑140 (MQ=255)                                                       ggTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGGGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCa                                                                             <  2:786157/140‑1 (MQ=255)                                                          ttCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCg                                                                                          >  1:1023387/1‑124 (MQ=255)                                                          ttCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCg                                                                                          <  2:1023387/124‑1 (MQ=255)                                                                   cAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCt                                                                 >  1:435518/1‑140 (MQ=255)                                                                   cAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCt                                                                 >  1:545131/1‑140 (MQ=255)                                                                                    ccattttAAACACA‑AAAATAGCCTGGGGGTGTTTCGCGGGGGTGAAATTTGGCCGCCAGTCATGAAACCGAGGGGCTTTAATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTc                                                <  2:545131/136‑1 (MQ=255)                                                                                    cACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTc                                                >  1:402586/1‑140 (MQ=255)                                                                                     aCCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTcc                                               <  2:1033952/140‑1 (MQ=255)                                                                                     aCCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTcc                                               >  2:182591/1‑140 (MQ=255)                                                                                      ccTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcg                                              <  1:58558/140‑1 (MQ=255)                                                                                      ccTTAAAACGCC‑AGAACCGCCGGGCGGGCATTCGCCGGCGTAAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcg                                              <  2:955166/140‑1 (MQ=255)                                                                                       cTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcgc                                             >  1:22507/1‑140 (MQ=255)                                                                                       cTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcgc                                             >  1:1082167/1‑140 (MQ=255)                                                                                       cTTTAAACCCCAAGAACCGCCGGGGGGGTATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcg                                              <  2:318024/140‑1 (MQ=255)                                                                                        tttAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcgcg                                            <  1:951366/140‑1 (MQ=255)                                                                                        tttAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTAAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcgcg                                            <  1:1011785/140‑1 (MQ=255)                                                                                          tAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGcc                                          >  1:533840/1‑140 (MQ=255)                                                                                             ccccc‑AGAATCGCCTGGCGGGCTTTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCa                                       <  2:22507/137‑1 (MQ=255)                                                                                             ccccc‑AGAATCCCCTGGCGGGCATCCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCa                                       <  2:172057/137‑1 (MQ=255)                                                                                                 caaGAACCGCCTGGCGGGCATTCGCCGGCGTAAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATgg                                    <  2:96063/138‑1 (MQ=255)                                                                                                 c‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGa                                   >  1:867109/1‑140 (MQ=255)                                                                                                 c‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGa                                   >  2:258719/1‑140 (MQ=255)                                                                                                          ccTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcgcg                                            <  1:661044/123‑1 (MQ=255)                                                                                                          ccTGGCGGGCATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcgcg                                            >  2:661044/1‑123 (MQ=255)                                                                                                                gggCATTCGCGGGCGTAAATTCGGGCGGCCAGTCATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGaa                     <  2:867109/140‑1 (MQ=255)                                                                                                                   cATTCGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTg                  >  1:876128/1‑140 (MQ=255)                                                                                                                       cGCCGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTa              <  2:1034510/140‑1 (MQ=255)                                                                                                                         ccGGCGTGAAATCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAAc            <  2:533840/140‑1 (MQ=255)                                                                                                                                  aaTCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGc   <  2:402586/140‑1 (MQ=255)                                                                                                                                   aTCTGGCCGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCa  <  2:209480/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     GCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCC‑AGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCA  >  NC_000913/6121‑6389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑