Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,718,375	A→G	F224L (TTT→CTT)	yiaF ←	DUF3053 domain‑containing protein YiaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,718,375	0	A	G	100.0%	294.6 / NA	80	F224L (TTT→CTT)	yiaF	DUF3053 domain‑containing protein YiaF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/43);  total (37/43)

AAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCA  >  NC_000913/3718243‑3718509                                                                                                                                     |                                                                                                                                       aaGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGa                                                                                                                                 >  2:1157691/1‑140 (MQ=255)   ggTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATg                                                                                                                               >  2:990786/1‑140 (MQ=255)   ggTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATg                                                                                                                               <  1:379689/140‑1 (MQ=255)   ggTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATg                                                                                                                               <  2:345910/140‑1 (MQ=255)     ttGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCt                                                                                                                             <  2:1106536/140‑1 (MQ=255)      tGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCtg                                                                                                                            >  2:49795/1‑140 (MQ=255)              aCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTa                                                                                                                    <  2:141149/140‑1 (MQ=255)              aCTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTa                                                                                                                    <  1:1157691/140‑1 (MQ=255)                  tgtgAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATgg                                                                                                                >  2:1079746/1‑140 (MQ=255)                     gAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGgcgc                                                                                                             <  2:329082/140‑1 (MQ=255)                     gAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGgcgc                                                                                                             <  1:123554/140‑1 (MQ=255)                      aaTCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCa                                                                                                            <  2:750849/140‑1 (MQ=255)                            cACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCTGGTAATGGCGCAATCAGt                                                                                                      <  1:840086/140‑1 (MQ=255)                            cACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAAt                                                                                                          >  2:516545/1‑136 (MQ=255)                            cACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAAt                                                                                                          <  1:516545/136‑1 (MQ=255)                            cACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGt                                                                                                      <  2:641247/140‑1 (MQ=255)                            cACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGt                                                                                                      <  1:1079746/140‑1 (MQ=255)                              cGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGttt                                                                                                    <  1:1165318/140‑1 (MQ=255)                                 cgcgGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGt                                                                                                      <  2:441219/135‑1 (MQ=255)                                 cgcgGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGt                                                                                                      >  1:441219/1‑135 (MQ=255)                                   cgGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTtat                                                                                               <  2:714227/140‑1 (MQ=255)                                             cgcgACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGc                                                                                     <  2:263238/140‑1 (MQ=255)                                               cgACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCct                                                                                   >  1:719928/1‑140 (MQ=255)                                                gACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCctg                                                                                  >  2:1209545/1‑140 (MQ=255)                                                gACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCctg                                                                                  >  2:679029/1‑140 (MQ=255)                                                     cGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCtgtg                                                                             <  2:719928/140‑1 (MQ=255)                                                        agagTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAg                                                                          >  2:995849/1‑140 (MQ=255)                                                            tAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCgg                                                                      <  1:49795/140‑1 (MQ=255)                                                               aGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGaa                                                                   >  1:499787/1‑140 (MQ=255)                                                                    gATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGaa                                                                   >  1:1010194/1‑135 (MQ=255)                                                                    gATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGaa                                                                   <  2:1010194/135‑1 (MQ=255)                                                                    gATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGa                                                              >  1:135842/1‑140 (MQ=255)                                                                     aTAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGAt                                                             >  2:1105776/1‑140 (MQ=255)                                                                      tAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATg                                                            >  1:664188/1‑140 (MQ=255)                                                                                ggCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGa                                                  <  1:1105776/140‑1 (MQ=255)                                                                                  cTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACa                                                <  1:995849/140‑1 (MQ=255)                                                                                  cTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACa                                                <  1:1072871/140‑1 (MQ=255)                                                                                  cTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACa                                                <  2:173615/140‑1 (MQ=255)                                                                                    tttACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACaaa                                              >  1:1150277/1‑140 (MQ=255)                                                                                     tgtcgctTTATTGCGTTGCAATACAAGCTGTAGTCCAGGCCTGATGAACCGGCTGATGATGAGCCGGTGAGGGCGCAATCAGTGTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAGCTGGATGGAATGTGCGAAAAAg                                             <  2:820858/135‑1 (MQ=255)                                                                                       aCTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCt                                           <  1:885439/140‑1 (MQ=255)                                                                                       aCTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCt                                           <  1:1036393/140‑1 (MQ=255)                                                                                        ctctTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTc                                          <  2:499787/140‑1 (MQ=255)                                                                                           tttATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCa                                         >  2:120903/1‑138 (MQ=255)                                                                                           tttATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCa                                         <  1:120903/138‑1 (MQ=255)                                                                                           tttATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCAcc                                       >  2:797937/1‑140 (MQ=255)                                                                                           tttATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCAcc                                       >  2:307257/1‑140 (MQ=255)                                                                                           ttaaTTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCAcc                                       >  2:1211799/4‑140 (MQ=255)                                                                                            ttATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCt                                      <  2:135842/140‑1 (MQ=255)                                                                                                  ggTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCt                                           >  1:663964/1‑129 (MQ=255)                                                                                                  ggTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCt                                           <  2:663964/129‑1 (MQ=255)                                                                                                  ggTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTAc                                >  2:382914/1‑140 (MQ=255)                                                                                                  ggTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTAc                                >  2:1052340/1‑140 (MQ=255)                                                                                                   gTTGCAGTAACTGCTGTAGTCCAGGGCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCCCTTGCCTGCTGTGAAGTCTGGAACTGGATGCCATTTGAGACAAAGCACACCTGAGTGcc                               <  2:940915/140‑1 (MQ=255)                                                                                                   gTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCt                                      >  1:676933/1‑133 (MQ=255)                                                                                                   gTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCt                                      <  2:676933/133‑1 (MQ=255)                                                                                                     tGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCtt                             >  2:1028440/1‑140 (MQ=255)                                                                                                     tGCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCtt                             >  1:1222621/1‑140 (MQ=255)                                                                                                      gCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTg                            <  1:307257/140‑1 (MQ=255)                                                                                                      gCAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTg                            <  1:797937/140‑1 (MQ=255)                                                                                                       cAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCt                                           >  1:556604/1‑124 (MQ=255)                                                                                                       cAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCt                                           <  2:556604/124‑1 (MQ=255)                                                                                                       cAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGc                           >  1:584924/1‑140 (MQ=255)                                                                                                       cAGTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGc                           >  2:517691/1‑140 (MQ=255)                                                                                                         gTAACTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTg                         >  2:39920/1‑140 (MQ=255)                                                                                                           aaCTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAg                       <  2:1150277/140‑1 (MQ=255)                                                                                                            aCTGCTGTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGc                      <  2:584924/140‑1 (MQ=255)                                                                                                                ctgTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATg                  <  2:1222621/140‑1 (MQ=255)                                                                                                                 tgTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGt                 <  2:664188/140‑1 (MQ=255)                                                                                                                  gTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTa                <  1:511996/140‑1 (MQ=255)                                                                                                                  gTAGTCCAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTa                <  1:1052340/140‑1 (MQ=255)                                                                                                                       ccAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGtctc           <  1:1209545/140‑1 (MQ=255)                                                                                                                       ccAGGCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGtctc           <  1:382914/140‑1 (MQ=255)                                                                                                                          ggCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCaa        >  1:137879/1‑140 (MQ=255)                                                                                                                           gCCTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAAc       >  1:928036/1‑140 (MQ=255)                                                                                                                             cTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCa     <  1:1211799/140‑1 (MQ=255)                                                                                                                             cTGATTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCa     <  1:679029/140‑1 (MQ=255)                                                                                                                                aTTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCa  >  2:53811/1‑140 (MQ=255)                                                                                                                                aTTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCa  >  2:440678/1‑140 (MQ=255)                                                                                                                                aTTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCa  >  2:265195/1‑140 (MQ=255)                                                                                                                                aTTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCa  >  2:181306/1‑140 (MQ=255)                                                                                                                                aTTAAGCGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCa  >  1:84889/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       AAGGTTGAGAAGAACTGTGTGAATCCGCACGTCGCGGACTAAGCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAGGCCTGATTAAACGCCTGATGCTGTGCCGGTAATGGCGCAATCAGTTTGTTATATTCACTTGCCTGCTGTGAAGTCGGGAACTGGATGCCATTTGCGACAAAGCTCACCTGAGTACCTTGCTGAGCAATGTAGTCTCCAACCATCA  >  NC_000913/3718243‑3718509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑