Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,112,313	A→G	intergenic (‑99/‑2)	yiiQ ← / → yiiR	DUF1454 domain‑containing protein YiiQ/DUF805 domain‑containing protein YiiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,112,313	0	A	G	100.0%	294.6 / NA	80	intergenic (‑99/‑2)	yiiQ/yiiR	DUF1454 domain‑containing protein YiiQ/DUF805 domain‑containing protein YiiR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/46);  total (34/46)

GAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATA  >  NC_000913/4112177‑4112444                                                                                                                                         |                                                                                                                                    gAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCAt                                                                                                                                  <  2:516767/140‑1 (MQ=255)  aaCATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATg                                                                                                                                 >  1:642650/1‑140 (MQ=255)  aaCATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATg                                                                                                                                 >  1:287824/1‑140 (MQ=255)   aCATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGa                                                                                                                                >  1:1077447/1‑140 (MQ=255)    cATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGAc                                                                                                                               <  1:1097826/140‑1 (MQ=255)        agagAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATa                                                                                                                           <  2:642650/140‑1 (MQ=255)          agaAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACa                                                                                                                         >  1:503730/1‑140 (MQ=255)            aaaaaaCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGc                                                                                                                       <  2:180292/140‑1 (MQ=255)              aaaaCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCaa                                                                                                                     <  1:704258/140‑1 (MQ=255)                        acacCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATtct                                                                                                           <  2:503730/140‑1 (MQ=255)                            ccTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCAtt                                                                                                       >  1:265754/1‑140 (MQ=255)                             cTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCCCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCAttt                                                                                                      <  1:361580/140‑1 (MQ=255)                               ggCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTaa                                                                                                    <  1:203790/140‑1 (MQ=255)                               ggCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTaa                                                                                                    <  2:1077447/140‑1 (MQ=255)                               ggCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTaa                                                                                                    <  1:977800/140‑1 (MQ=255)                                 cTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAg                                                                                                  >  1:936348/1‑140 (MQ=255)                                  ttCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAgg                                                                                                 <  2:941558/140‑1 (MQ=255)                                  ttCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAgg                                                                                                 <  2:1010384/140‑1 (MQ=255)                                    cATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGc                                                                                               >  1:411428/1‑140 (MQ=255)                                      ttGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGt                                                                                             >  1:207888/1‑140 (MQ=255)                                      ttGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGt                                                                                             >  2:1149516/1‑140 (MQ=255)                                          cAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTg                                                                                         >  1:424032/1‑140 (MQ=255)                                           aGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTgg                                                                                        >  2:619979/1‑140 (MQ=255)                                           aGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTgg                                                                                        <  1:1149516/140‑1 (MQ=255)                                             tACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGAc                                                                                      >  2:697835/1‑140 (MQ=255)                                               ccTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGc                                                                                    >  2:831473/1‑140 (MQ=255)                                                cTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGcc                                                                                   >  2:239872/1‑140 (MQ=255)                                                  tttGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGt                                                                                 <  2:936348/140‑1 (MQ=255)                                                   ttGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTg                                                                                >  2:782596/1‑140 (MQ=255)                                                    tGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGa                                                                               >  2:152599/1‑140 (MQ=255)                                                    tGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGa                                                                               >  2:1162651/1‑140 (MQ=255)                                                       tATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGAttt                                                                            <  2:1159126/140‑1 (MQ=255)                                                         tGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCt                                                                          >  2:73744/1‑140 (MQ=255)                                                          gACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTAtt                                                                                          <  2:960588/123‑1 (MQ=255)                                                          gACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTAtt                                                                                          >  1:960588/1‑123 (MQ=255)                                                           aCTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTgg                                                                        <  2:287824/140‑1 (MQ=255)                                                             tGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGAt                                                                      <  1:73744/140‑1 (MQ=255)                                                             tGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGAt                                                                      <  1:697835/140‑1 (MQ=255)                                                             tGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGAt                                                                      <  2:424032/140‑1 (MQ=255)                                                                cTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTg                                                                   >  1:1021281/1‑140 (MQ=255)                                                                  ccGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGa                                                                 <  1:653942/140‑1 (MQ=255)                                                                  ccGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGa                                                                 <  2:230569/140‑1 (MQ=255)                                                                  ccGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGa                                                                 <  1:239872/140‑1 (MQ=255)                                                                  ccGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGa                                                                 <  2:705682/140‑1 (MQ=255)                                                                  ccGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGa                                                                 <  1:782596/140‑1 (MQ=255)                                                                     aTTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGAt                                                                      >  2:747546/1‑132 (MQ=255)                                                                     aTTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGAt                                                                      <  1:747546/132‑1 (MQ=255)                                                                     aTTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAg                                                              <  2:484198/140‑1 (MQ=255)                                                                     aTTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAg                                                              >  1:602458/1‑140 (MQ=255)                                                                     aTTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAg                                                              >  2:597381/1‑140 (MQ=255)                                                                             gATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGa                                                                 <  2:734331/129‑1 (MQ=255)                                                                             gATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGa                                                                 >  1:734331/1‑129 (MQ=255)                                                                                  cTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCGAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGc                                                 <  2:1132930/140‑1 (MQ=255)                                                                                       gCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCa                                            >  2:581308/1‑140 (MQ=255)                                                                                       gCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCa                                            <  1:152599/140‑1 (MQ=255)                                                                                         ccTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAgg                                              >  2:917448/1‑136 (MQ=255)                                                                                         ccTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAgg                                              <  1:917448/136‑1 (MQ=255)                                                                                            tGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTg                                       <  1:1162651/140‑1 (MQ=255)                                                                                               cAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTg                                    <  1:581308/140‑1 (MQ=255)                                                                                                 aaaGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCt                                  <  2:602458/140‑1 (MQ=255)                                                                                                       tAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTtctc                            >  1:1113029/1‑140 (MQ=255)                                                                                                       tAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTtctc                            >  2:385079/1‑140 (MQ=255)                                                                                                       tAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTtctc                            >  1:803615/1‑140 (MQ=255)                                                                                                       tAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATT‑GACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCa                           >  1:1097368/1‑140 (MQ=255)                                                                                                        aGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCa                           >  1:544653/1‑140 (MQ=255)                                                                                                           aTCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTg                        <  2:544653/140‑1 (MQ=255)                                                                                                           aTCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATT‑GACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTgg                       <  2:1097368/140‑1 (MQ=255)                                                                                                            tCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTgg                       <  2:265754/140‑1 (MQ=255)                                                                                                            tCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTgg                       <  1:1018244/140‑1 (MQ=255)                                                                                                             ccTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGc                      <  1:408719/140‑1 (MQ=255)                                                                                                             ccTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGc                      <  2:1169039/140‑1 (MQ=255)                                                                                                              cTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCg                     <  2:1021281/140‑1 (MQ=255)                                                                                                                ggAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCggg                   <  1:831473/140‑1 (MQ=255)                                                                                                                  aaaaGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTa                 <  2:207888/140‑1 (MQ=255)                                                                                                                         aaCCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCt          <  2:411428/140‑1 (MQ=255)                                                                                                                           ccATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTAc        >  2:467961/1‑140 (MQ=255)                                                                                                                            cATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACt       <  2:803615/140‑1 (MQ=255)                                                                                                                             aTCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTc      <  1:372743/140‑1 (MQ=255)                                                                                                                                aaGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGat   >  1:628486/1‑140 (MQ=255)                                                                                                                                 aGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGata  >  1:828322/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    GAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATA  >  NC_000913/4112177‑4112444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑