Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,465,380	C→T	L267L (CTG→CTA)	treB ←	trehalose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,465,380	0	C	T	100.0%	215.3 / NA	60	L267L (CTG→CTA)	treB	trehalose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/36);  total (24/36)

GAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATG  >  NC_000913/4465248‑4465515                                                                                                                                     |                                                                                                                                        gAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAAc                                                                                                                                  <  1:333043/140‑1 (MQ=255)  aaGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGaa                                                                                                                                   >  1:893874/1‑138 (MQ=255)  aaGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGaa                                                                                                                                   <  2:893874/138‑1 (MQ=255)  aaGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACa                                                                                                                                 <  1:12387/140‑1 (MQ=255)      cAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACg                                                                                                                             >  2:536090/1‑140 (MQ=255)      cAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACg                                                                                                                             >  1:172041/1‑140 (MQ=255)       aaaCAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACACCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACgg                                                                                                                            >  1:267284/1‑140 (MQ=255)        aaCAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACggg                                                                                                                           <  2:296585/140‑1 (MQ=255)         aCAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGt                                                                                                                          <  2:633482/140‑1 (MQ=255)         aCAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGt                                                                                                                          <  2:532771/140‑1 (MQ=255)           aaTGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTac                                                                                                                        <  1:561707/140‑1 (MQ=255)           aaTGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTac                                                                                                                        <  2:625246/140‑1 (MQ=255)           aaTGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTac                                                                                                                        <  2:1216260/140‑1 (MQ=255)                tgcGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTaccacca                                                                                                                   <  1:1091654/140‑1 (MQ=255)                          ggAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATagag                                                                                                         <  2:862539/140‑1 (MQ=255)                           gAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAgg                                                                                                        <  1:667510/140‑1 (MQ=255)                            aGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGt                                                                                                       >  1:248568/1‑140 (MQ=255)                                 aGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATcc                                                                                                  <  2:102423/140‑1 (MQ=255)                                      ccGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCAc                                                                                             <  1:1034580/140‑1 (MQ=255)                                        ggTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGa                                                                                           <  2:223084/140‑1 (MQ=255)                                         gTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGAt                                                                                          <  1:1062558/140‑1 (MQ=255)                                                   gACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAgg                                                                                <  1:164782/140‑1 (MQ=255)                                                   gACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAgg                                                                                <  1:53666/140‑1 (MQ=255)                                                   gACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAgg                                                                                <  2:745081/140‑1 (MQ=255)                                                   gACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAgg                                                                                <  1:623448/140‑1 (MQ=255)                                                   gACGTACCGCAAAGGCAACACCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAgg                                                                                <  2:267284/140‑1 (MQ=255)                                                    aCGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGc                                                                               <  1:209601/140‑1 (MQ=255)                                                     cGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCg                                                                              <  1:912187/140‑1 (MQ=255)                                                        aCCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGt                                                                           >  2:1224086/1‑140 (MQ=255)                                                                        cATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAgc                                                           <  2:522868/140‑1 (MQ=255)                                                                         aTCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAgcg                                                          <  1:1140458/140‑1 (MQ=255)                                                                          tCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAgcgc                                                         >  1:1030413/1‑140 (MQ=255)                                                                           cGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGcc                                                        >  2:845632/1‑140 (MQ=255)                                                                              cAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCAAGt                                                     <  2:1030413/140‑1 (MQ=255)                                                                                    tGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCt                                               >  1:141971/1‑140 (MQ=255)                                                                                    tGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCt                                               >  2:560129/1‑140 (MQ=255)                                                                                    tGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCt                                               >  2:1200143/1‑140 (MQ=255)                                                                                           aaaCGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTg                                        <  1:536090/140‑1 (MQ=255)                                                                                                           gcATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTgcgc                        >  2:1055764/1‑140 (MQ=255)                                                                                                            cATGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGcc                       <  1:376140/140‑1 (MQ=255)                                                                                                             aTGGGCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCt                      <  1:1161792/140‑1 (MQ=255)                                                                                                                ggCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGAt                   >  1:653659/1‑140 (MQ=255)                                                                                                                ggCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGAt                   >  2:434134/1‑140 (MQ=255)                                                                                                                ggCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGAt                   >  1:526594/1‑140 (MQ=255)                                                                                                                ggCGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGAt                   >  2:1189075/1‑140 (MQ=255)                                                                                                                  cGAGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAg                 <  1:1100768/140‑1 (MQ=255)                                                                                                                    aGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGcc               >  2:638265/1‑140 (MQ=255)                                                                                                                    aGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGcc               >  2:1067974/1‑140 (MQ=255)                                                                                                                    aGGAACACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGcc               >  1:924098/1‑140 (MQ=255)                                                                                                                       aaCACCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTAc            >  1:197299/1‑140 (MQ=255)                                                                                                                         cacCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGcc               >  1:1031700/1‑135 (MQ=255)                                                                                                                         cacCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGcc               <  2:1031700/135‑1 (MQ=255)                                                                                                                          acCGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACttt         >  2:357022/1‑140 (MQ=255)                                                                                                                           ccGCGAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTg        >  1:385886/1‑140 (MQ=255)                                                                                                                            cgcgAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTgg       <  2:330901/140‑1 (MQ=255)                                                                                                                            cgcgAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTgg       <  2:141971/140‑1 (MQ=255)                                                                                                                            cgcgAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTgg       <  1:560129/140‑1 (MQ=255)                                                                                                                            cgcgAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTgg       <  1:845632/140‑1 (MQ=255)                                                                                                                             gcgAGGATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGc      <  1:357022/140‑1 (MQ=255)                                                                                                                                 ggATTAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATg  >  1:140422/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        GAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGATCAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATG  >  NC_000913/4465248‑4465515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑