Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	302,507	A→G	L23L (TTG→CTG)	paoA ←	aldehyde dehydrogenase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	302,507	0	A	G	100.0%	110.9 / NA	32	L23L (TTG→CTG)	paoA	aldehyde dehydrogenase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/21);  total (11/21)

CACCTTCAGTGTCAGGGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACG  >  NC_000913/302373‑302642                                                                                                                                       |                                                                                                                                           cACCTTCAGTGTCAGGGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCCCTGTTGCGGCACTCCCTTTTATCAGATCGCGACGGGGCAGACTTa                                                                                                                                       >  2:899572/1‑140 (MQ=255)         gtgtCAGGGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTTCGGCGCTCACTTTTATCAAATCCCGACGGGGCAGACTTTAATAGTgg                                                                                                                               >  2:427144/1‑139 (MQ=255)          tgtCAGGGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCCCCGTTTCGGGGCCCCCCTTTATTAAAACGCGACCGGGCAGACTTAAAACGTggg                                                                                                                              >  2:546766/1‑138 (MQ=255)            tCAGGGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGc                                                                                                                            <  1:691888/140‑1 (MQ=255)              aGGGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTTGCCGGCGTTGGTGGGCGCACCTTAATTAGAACGCGGGGGGGCCGAAATTAATCGCGCGGCTc                                                                                                                          >  2:831915/1‑140 (MQ=255)                 ggCATTATCTCTGGTGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTg                                                                                                                      <  2:519038/140‑1 (MQ=255)                 ggCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTg                                                                                                                      <  1:427144/140‑1 (MQ=255)                             ggCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCCGTTTCGGGGCTCACTTTTATCAGATCGCGACGGGGCAGACTTAAAACGTGCGGGTC‑CGG‑CGTTCCCAcccc                                                                                                         >  2:630514/1‑140 (MQ=255)                             ggCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGGTGCCGCCGTTGGGGCGCTCCCTTTAATCAAAACGCGACGGGGCAGACTTAAAATTGGCGGGCC‑GTGCC‑TTCCCAAccc                                                                                                         >  2:1038049/1‑140 (MQ=255)                                            gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTa                                                                                         <  2:866332/140‑1 (MQ=255)                                                  aCGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCCGTTGCGGGGCTCCCTTTTATAAGATCGCGACGGGGTAGAACTAAAACGTGCGGGTCGGGG‑C‑TCCCCAACCC‑ATTATCTTTCGGGGATTCggc                                                                                   >  2:1177686/1‑138 (MQ=255)                                                      ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTgg                                                                               <  1:95869/140‑1 (MQ=255)                                                      ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTgg                                                                               <  1:871581/140‑1 (MQ=255)                                                      ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTgg                                                                               <  1:630514/140‑1 (MQ=255)                                                       tGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGt                                                                              <  1:831915/140‑1 (MQ=255)                                                           gccAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCt                                                                          <  1:683558/140‑1 (MQ=255)                                                                cGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATAc                                                                     <  2:29923/140‑1 (MQ=255)                                                                        gAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTc                                                             <  2:1131552/140‑1 (MQ=255)                                                                        gAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTc                                                             <  1:290113/140‑1 (MQ=255)                                                                                     ggcggTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAg                                                <  1:1052919/140‑1 (MQ=255)                                                                                       cggTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAggg                                              <  2:105936/140‑1 (MQ=255)                                                                                                cTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAag                                     <  1:546766/140‑1 (MQ=255)                                                                                                           cTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTg                                 <  1:1221656/133‑1 (MQ=255)                                                                                                           cTCACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTg                                 >  2:1221656/1‑133 (MQ=255)                                                                                                             cACTTTAATCAGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCgag                        >  1:113287/1‑140 (MQ=255)                                                                                                                       aGATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATg              <  1:1038049/140‑1 (MQ=255)                                                                                                                        gATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGc             >  1:81253/1‑140 (MQ=255)                                                                                                                        gATCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGc             >  1:905675/1‑140 (MQ=255)                                                                                                                         aTCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCt            >  1:405789/1‑140 (MQ=255)                                                                                                                          tCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCtt           >  1:514556/1‑140 (MQ=255)                                                                                                                          tCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCtt           >  2:443058/1‑140 (MQ=255)                                                                                                                          tCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCtt           >  2:537445/1‑140 (MQ=255)                                                                                                                          tCGCGACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCtt           >  2:736027/1‑140 (MQ=255)                                                                                                                              gACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCtttttt       <  1:443058/140‑1 (MQ=255)                                                                                                                              gACGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCtttttt       >  2:1049337/1‑140 (MQ=255)                                                                                                                                cGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTaa     <  1:736027/140‑1 (MQ=255)                                                                                                                                cGGGTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTaa     <  1:1177686/140‑1 (MQ=255)                                                                                                                                   gTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACg  >  2:1169531/1‑140 (MQ=255)                                                                                                                                   gTCAGACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACg  <  1:1169531/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                           CACCTTCAGTGTCAGGGGCATTATCTCTGGCGCGGGTGTAGCTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTC‑GTG‑C‑TTCCCAACCCGATTATCTTCGGGGTATTCGCCTTGGTTGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACG  >  NC_000913/302373‑302642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑