Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	450,072	C→T	W190* (TGG→TGA)	cyoB ←	cytochrome bo3 ubiquinol oxidase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	450,072	0	C	T	100.0%	312.5 / NA	86	W190* (TGG→TGA)	cyoB	cytochrome bo3 ubiquinol oxidase subunit 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/40);  total (46/40)

TCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGC  >  NC_000913/449936‑450208                                                                                                                                         |                                                                                                                                         tCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAg                                                                                                                                       >  2:478518/1‑140 (MQ=255) tCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAg                                                                                                                                       >  1:510347/1‑140 (MQ=255)  cgcgCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGt                                                                                                                                      >  1:1023168/1‑140 (MQ=255)  cgcgCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGt                                                                                                                                      >  2:963340/1‑140 (MQ=255)   gcgcACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTa                                                                                                                                     >  2:524884/1‑140 (MQ=255)    cgcACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTaa                                                                                                                                    >  1:646112/1‑140 (MQ=255)          gTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGaca                                                                                                                              >  1:710641/1‑140 (MQ=255)          gTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGaca                                                                                                                              <  1:944530/140‑1 (MQ=255)          gTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGaca                                                                                                                              >  2:32135/1‑140 (MQ=255)           tGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGacac                                                                                                                             >  2:721856/1‑140 (MQ=255)            gATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACAcc                                                                                                                            <  2:430575/140‑1 (MQ=255)            gATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACAcc                                                                                                                            <  1:746279/140‑1 (MQ=255)            gATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACAcc                                                                                                                            <  2:545261/140‑1 (MQ=255)               gCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGAc                                                                                                                         >  2:689055/1‑140 (MQ=255)                  cAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTcc                                                                                                                      <  2:732648/140‑1 (MQ=255)                   aGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTccc                                                                                                                     >  2:1016897/1‑140 (MQ=255)                   aGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTccc                                                                                                                     <  1:782079/140‑1 (MQ=255)                   aGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTccc                                                                                                                     >  1:744810/1‑140 (MQ=255)                   aGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTccc                                                                                                                     >  2:782079/1‑140 (MQ=255)                   aGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCA‑CTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCg                                                                                                                    <  2:1225128/140‑1 (MQ=255)                    ggTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCg                                                                                                                    <  1:524884/140‑1 (MQ=255)                    ggTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCg                                                                                                                    <  1:829230/140‑1 (MQ=255)                     gTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACAcc                                                                                                                            >  1:551059/1‑131 (MQ=255)                     gTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACAcc                                                                                                                            <  1:590324/131‑1 (MQ=255)                     gTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACAcc                                                                                                                            <  2:551059/131‑1 (MQ=255)                     gTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACAcc                                                                                                                            >  2:590324/1‑131 (MQ=255)                     gTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCgg                                                                                                                   >  1:799073/1‑140 (MQ=255)                      tAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGa                                                                                                                  <  1:478518/140‑1 (MQ=255)                      tAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGa                                                                                                                  <  2:320547/140‑1 (MQ=255)                      tAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGa                                                                                                                  <  2:510347/140‑1 (MQ=255)                      tAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGa                                                                                                                  <  2:572697/140‑1 (MQ=255)                      tAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTAAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGa                                                                                                                  <  1:1051975/140‑1 (MQ=255)                       aaaTACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGAc                                                                                                                 <  1:1016897/140‑1 (MQ=255)                        aaTACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACt                                                                                                                <  1:689055/140‑1 (MQ=255)                         aTACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTg                                                                                                               <  2:168057/140‑1 (MQ=255)                          tACTGGCATCTTGAACATGGGCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGt                                                                                                              >  2:746236/1‑140 (MQ=255)                             tGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTAct                                                                                                           <  2:646112/140‑1 (MQ=255)                             tGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTAct                                                                                                           <  2:744810/140‑1 (MQ=255)                               gCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTActct                                                                                                         >  2:1092723/1‑140 (MQ=255)                               gCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGCAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTActct                                                                                                         >  1:1222454/1‑140 (MQ=255)                                cATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTa                                                                                                        <  2:995265/140‑1 (MQ=255)                                            ggTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCg                                                                                            <  2:1023168/140‑1 (MQ=255)                                            ggTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCg                                                                                            <  1:1092723/140‑1 (MQ=255)                                             gTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCgg                                                                                           >  2:945978/1‑140 (MQ=255)                                              tCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTTCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGt                                                                                          >  1:260147/1‑140 (MQ=255)                                              tCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGt                                                                                          >  1:948368/1‑140 (MQ=255)                                              tCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGt                                                                                          >  1:1070586/1‑140 (MQ=255)                                              tCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGt                                                                                          >  1:291580/1‑140 (MQ=255)                                                   cccGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATa                                                                                     >  1:976960/1‑140 (MQ=255)                                                        tGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCg                                                                                            >  1:579660/1‑128 (MQ=255)                                                        tGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCg                                                                                            <  2:579660/128‑1 (MQ=255)                                                        tGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgcca                                                                                >  1:947040/1‑140 (MQ=255)                                                        tGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgcca                                                                                >  2:1115288/1‑140 (MQ=255)                                                        tGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgcca                                                                                >  2:794654/1‑140 (MQ=255)                                                        tGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgcca                                                                                >  1:80398/1‑140 (MQ=255)                                                            cgcATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagcca                                                                            >  1:176677/1‑140 (MQ=255)                                                                  ttCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGt                                                                      <  2:775780/140‑1 (MQ=255)                                                                         tGGTAACGAAGAAGTTGATACCGGCAAGCGTCGTACCTATACCGGATAGCTGGAGACTTCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCa                                                               >  2:134211/1‑140 (MQ=255)                                                                            tAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGACTGCGCAAAt                                                            >  1:845081/1‑140 (MQ=255)                                                                                 aagaagTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGcc                                                       <  2:948368/140‑1 (MQ=255)                                                                                   gaagTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGcc                                                       >  2:1062791/1‑138 (MQ=255)                                                                                   gaagTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGcc                                                       <  1:1062791/138‑1 (MQ=255)                                                                                       ttGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGcc                                                 <  2:799073/140‑1 (MQ=255)                                                                                                     cgtcgtACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTaa                                   <  1:945978/140‑1 (MQ=255)                                                                                                      gtcgtACCTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAAc                                  <  1:721856/140‑1 (MQ=255)                                                                                                            ccTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAAt                            <  2:399861/140‑1 (MQ=255)                                                                                                            ccTATACCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAAt                            <  1:28044/140‑1 (MQ=255)                                                                                                               ataCCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATcac                         >  2:862564/1‑140 (MQ=255)                                                                                                                taCCGGATCGCTGCAGCCTCCATATCAAGTAAACGACACCGACTCCCGGACAGTACTCTATCCCCGATAGCGGTGGATAGGCCAGCCAGCTGGTGTTCGAAATTTCGCCCGCGCCGAGAGAAACGTTATCCAGAATcaca                        <  2:1222454/140‑1 (MQ=255)                                                                                                                taCCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATcaca                        <  1:32135/140‑1 (MQ=255)                                                                                                                taCCGGATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATcaca                        >  2:447349/1‑140 (MQ=255)                                                                                                                     gATAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaac                   >  1:28361/1‑140 (MQ=255)                                                                                                                     gATAACTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaac                   >  1:557529/1‑140 (MQ=255)                                                                                                                      aTAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaaca                  >  1:450114/1‑140 (MQ=255)                                                                                                                       tAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaacaa                 >  2:863809/1‑140 (MQ=255)                                                                                                                       tAGCTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaacaa                 >  1:252602/1‑140 (MQ=255)                                                                                                                          cTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACgg              >  1:465690/1‑140 (MQ=255)                                                                                                                          cTGGAGACTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACgg              <  1:1115288/140‑1 (MQ=255)                                                                                                                             gagaCTCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTaa           >  1:1215515/1‑140 (MQ=255)                                                                                                                                  tCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAg      >  1:372164/1‑140 (MQ=255)                                                                                                                                  tCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAg      <  2:80398/140‑1 (MQ=255)                                                                                                                                  tCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAg      >  1:719620/1‑140 (MQ=255)                                                                                                                                  tCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAg      <  2:947040/140‑1 (MQ=255)                                                                                                                                  tCCATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAg      >  2:528127/1‑140 (MQ=255)                                                                                                                                   ccATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGa     <  2:710641/140‑1 (MQ=255)                                                                                                                                    cATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGaa    >  2:39041/1‑140 (MQ=255)                                                                                                                                    cATATTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGaa    <  1:963340/140‑1 (MQ=255)                                                                                                                                      tatTCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGc  <  2:557529/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGC  >  NC_000913/449936‑450208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑