Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	527,577	T→C	intergenic (+36/‑4)	rhsD → / → ybbC	protein RhsD/PF15631 family protein YbbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	527,577	0	T	C	100.0%	301.0 / NA	82	intergenic (+36/‑4)	rhsD/ybbC	protein RhsD/PF15631 family protein YbbC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/39);  total (43/39)

CGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT  >  NC_000913/527449‑527713                                                                                                                                 |                                                                                                                                         cGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAata                                                                                                                               >  1:106007/1‑140 (MQ=255) cGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAata                                                                                                                               <  2:854111/140‑1 (MQ=255)    tttGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAg                                                                                                                            <  2:996074/140‑1 (MQ=255)      tGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGtt                                                                                                                          >  2:324865/1‑140 (MQ=255)              ttAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTATTTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATAttt                                                                                                                  >  2:1104330/1‑140 (MQ=255)               taataataaAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATAtttt                                                                                                                 >  1:1136256/1‑140 (MQ=255)                       aaGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCtt                                                                                                         >  1:466699/1‑140 (MQ=255)                       aaGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCtt                                                                                                         >  2:1046446/1‑140 (MQ=255)                        aGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        <  2:466699/140‑1 (MQ=255)                        aGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        >  1:1224499/1‑140 (MQ=255)                        aGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        >  1:1129243/1‑140 (MQ=255)                          gagaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCa                                                                                                      <  2:748823/140‑1 (MQ=255)                            gaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        <  1:99724/136‑1 (MQ=255)                            gaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        >  2:99724/1‑136 (MQ=255)                            gaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAtt                                                                                                    >  2:1234883/1‑140 (MQ=255)                            gaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAtt                                                                                                    >  1:815794/1‑140 (MQ=255)                             aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        >  2:683692/1‑135 (MQ=255)                             aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCttt                                                                                                        <  1:683692/135‑1 (MQ=255)                             aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttt                                                                                                   >  2:1188809/1‑140 (MQ=255)                             aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttt                                                                                                   >  1:1076811/1‑140 (MQ=255)                                atatTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttttta                                                                                                <  1:1171806/140‑2 (MQ=255)                                atatTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAttttta                                                                                                <  2:106007/140‑2 (MQ=255)                                 tatTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAtttttat                                                                                               <  1:1104330/140‑3 (MQ=255)                                  atTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCAtttttata                                                                                              <  1:1046446/140‑4 (MQ=255)                                    ttttATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACt                                                                                           >  2:694731/1‑140 (MQ=255)                                          cAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGc                                                                                     <  1:1234883/140‑1 (MQ=255)                                          cAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGc                                                                                     <  1:324865/140‑1 (MQ=255)                                           aGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGcc                                                                                    <  1:1188809/140‑1 (MQ=255)                                           aGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGcc                                                                                    <  2:815794/140‑1 (MQ=255)                                             aaaTAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTg                                                                                      <  2:981968/136‑1 (MQ=255)                                             aaaTAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTg                                                                                      >  1:981968/1‑136 (MQ=255)                                                tAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTaa                                                                               <  1:1092113/140‑1 (MQ=255)                                                    gtCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATgag                                                                           >  2:833258/1‑140 (MQ=255)                                                     tCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATgaga                                                                          >  2:162074/1‑140 (MQ=255)                                                       ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTAtt                                                                                        <  2:1173488/124‑1 (MQ=255)                                                       ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTAtt                                                                                        >  1:1173488/1‑124 (MQ=255)                                                       ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGcc                                                                                    >  2:624754/1‑128 (MQ=255)                                                       ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGcc                                                                                    <  1:624754/128‑1 (MQ=255)                                                       ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATgaga                                                                          <  2:570240/138‑1 (MQ=255)                                                       ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATgaga                                                                          >  1:570240/1‑138 (MQ=255)                                                       ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACa                                                                        >  1:20907/1‑140 (MQ=255)                                                       ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACa                                                                        >  2:743968/1‑140 (MQ=255)                                                       ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTCATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACa                                                                        >  2:524982/1‑140 (MQ=255)                                                        cGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAGATATAGTTGAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAg                                                                       <  2:417909/140‑1 (MQ=255)                                                        cGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAg                                                                       <  1:162074/140‑1 (MQ=255)                                                               gAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa                                                                <  1:784474/140‑1 (MQ=255)                                                                          gCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa                                                                >  2:589941/1‑129 (MQ=255)                                                                          gCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa                                                                <  1:589941/129‑1 (MQ=255)                                                                           cATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCtgatga                                                    <  2:1136256/140‑1 (MQ=255)                                                                               ccGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAAc                                                >  2:272364/1‑140 (MQ=255)                                                                                 gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACat                                              >  1:202899/1‑140 (MQ=255)                                                                                 gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACat                                              >  2:888966/1‑140 (MQ=255)                                                                                 gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACat                                              >  1:1220602/1‑140 (MQ=255)                                                                                 gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACat                                              >  2:910861/1‑140 (MQ=255)                                                                                  tATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatt                                             >  1:258849/1‑140 (MQ=255)                                                                                  tATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatt                                             >  2:554132/1‑140 (MQ=255)                                                                                   aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGaaaa                                                 <  2:1049303/135‑1 (MQ=255)                                                                                   aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGaaaa                                                 >  1:1049303/1‑135 (MQ=255)                                                                                   aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatta                                            >  2:1095293/1‑140 (MQ=255)                                                                                   aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatta                                            >  2:192277/1‑140 (MQ=255)                                                                                    tCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa                                               >  1:1000830/1‑136 (MQ=255)                                                                                    tCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa                                               <  2:1000830/136‑1 (MQ=255)                                                                                     cATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatta                                            <  1:773501/138‑1 (MQ=255)                                                                                     cATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatta                                            >  2:773501/1‑138 (MQ=255)                                                                                        gATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTAttttt                                       >  2:465759/1‑140 (MQ=255)                                                                                         aTTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTa                                      >  2:1115210/1‑140 (MQ=255)                                                                                         aTTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTa                                      <  1:1115210/140‑1 (MQ=255)                                                                                          ttAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTAt                                     <  2:202899/140‑1 (MQ=255)                                                                                                 tAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTAt                              <  2:1220602/140‑1 (MQ=255)                                                                                                                 ggggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  1:694731/140‑1 (MQ=255)                                                                                                                 ggggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  2:258849/140‑1 (MQ=255)                                                                                                                 ggggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  1:192277/140‑1 (MQ=255)                                                                                                                  gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAttt             >  2:952422/1‑140 (MQ=255)                                                                                                                  gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAttt             >  2:879504/1‑140 (MQ=255)                                                                                                                     ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  2:807882/136‑1 (MQ=255)                                                                                                                     ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              >  1:807882/1‑136 (MQ=255)                                                                                                                     ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              <  1:263643/136‑1 (MQ=255)                                                                                                                     ccTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt              >  2:263643/1‑136 (MQ=255)                                                                                                                         taAGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATaaa      <  1:833258/140‑1 (MQ=255)                                                                                                                           aGGAGACTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATa    <  2:20907/140‑1 (MQ=255)                                                                                                                             gagaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  1:910861/140‑1 (MQ=255)                                                                                                                             gagaCTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct  <  2:1076811/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         CGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT  >  NC_000913/527449‑527713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑