Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	668,817	T→C	S74G (AGC→GGC)	rsfS ←	ribosomal silencing factor RsfS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	668,817	0	T	C	100.0%	220.9 / NA	62	S74G (AGC→GGC)	rsfS	ribosomal silencing factor RsfS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/33);  total (29/33)

CATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGATTTGCC  >  NC_000913/668680‑668952                                                                                                                                          |                                                                                                                                        cATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGt                                                                                                                                       >  1:1079518/1‑140 (MQ=255) cATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGt                                                                                                                                       >  1:472782/1‑140 (MQ=255)     ttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTc                                                                                                                                   <  2:1165433/140‑1 (MQ=255)             cACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTa                                                                                                                           >  1:147068/1‑140 (MQ=255)                ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGc                                                                                                                        <  1:924364/140‑1 (MQ=255)                                  cACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCg                                                                                                      <  1:1030253/140‑1 (MQ=255)                                     gCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTg                                                                                                   <  2:744339/140‑1 (MQ=255)                                          aaCTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCga                                                                                              <  2:147068/140‑1 (MQ=255)                                          aaCTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCga                                                                                              <  2:472782/140‑1 (MQ=255)                                               cAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTc                                                                                         <  1:96831/140‑1 (MQ=255)                                                 gagTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCt                                                                                       >  1:659320/1‑140 (MQ=255)                                                  agTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTg                                                                                      >  1:695621/1‑140 (MQ=255)                                                      tttCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGcaca                                                                                  >  1:949728/1‑140 (MQ=255)                                                            gTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTgg                                                                            >  1:951757/1‑140 (MQ=255)                                                              tCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTc                                                                          >  2:898684/1‑140 (MQ=255)                                                                  aCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCa                                                                      >  2:534082/1‑140 (MQ=255)                                                                  aCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCa                                                                      >  2:560341/1‑140 (MQ=255)                                                                  aCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCa                                                                      <  1:1073360/140‑1 (MQ=255)                                                                  aCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCa                                                                      <  1:898684/140‑1 (MQ=255)                                                                   cAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCaa                                                                     <  1:744927/140‑1 (MQ=255)                                                                       cGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGa                                                                 <  2:659320/140‑1 (MQ=255)                                                                       cGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGa                                                                 <  2:867564/140‑1 (MQ=255)                                                                       cGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGa                                                                 >  1:867564/1‑140 (MQ=255)                                                                         aCGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACa                                                               >  1:529178/1‑140 (MQ=255)                                                                         aCGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACa                                                               >  1:612073/1‑140 (MQ=255)                                                                                tctTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATg                                                        >  2:243416/1‑140 (MQ=255)                                                                                         aTGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGa                                               <  2:529178/140‑1 (MQ=255)                                                                                         aTGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACATTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGa                                               <  2:1079518/140‑1 (MQ=255)                                                                                          tGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGAc                                              >  2:380085/1‑140 (MQ=255)                                                                                          tGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGAc                                              >  2:1009877/1‑140 (MQ=255)                                                                                             cATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTa                                           <  2:951757/140‑1 (MQ=255)                                                                                                ggACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTAccc                                        <  1:1130778/140‑1 (MQ=255)                                                                                                ggACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTAccc                                        >  2:561030/1‑140 (MQ=255)                                                                                                ggACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTAccc                                        >  2:322878/1‑140 (MQ=255)                                                                                                 gACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACgg                                                    >  1:23688/1‑127 (MQ=255)                                                                                                 gACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACgg                                                    <  2:23688/127‑1 (MQ=255)                                                                                                 gACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCg                                       <  1:104431/140‑1 (MQ=255)                                                                                                  aCAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGt                                      >  1:500627/1‑140 (MQ=255)                                                                                                    aaTCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTAc                                    <  1:560341/140‑1 (MQ=255)                                                                                                    aaTCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTAc                                    <  1:534082/140‑1 (MQ=255)                                                                                                    aaTCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTAc                                    <  1:860420/140‑1 (MQ=255)                                                                                                      tCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGt                                            >  1:54187/1‑130 (MQ=255)                                                                                                      tCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGt                                            <  2:54187/130‑1 (MQ=255)                                                                                                      tCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAg                                  >  1:723684/1‑140 (MQ=255)                                                                                                      tCACATCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAg                                  >  1:731738/1‑140 (MQ=255)                                                                                                       cacaTCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAga                                 >  2:668724/1‑140 (MQ=255)                                                                                                           tCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGa                                               >  1:1225552/1‑122 (MQ=255)                                                                                                           tCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGa                                               <  2:1225552/122‑1 (MQ=255)                                                                                                           tCGCCCAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAgatgat                             >  1:972988/1‑140 (MQ=255)                                                                                                               ccAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATg                         <  1:972074/140‑1 (MQ=255)                                                                                                               ccAAATCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATg                         <  1:668724/140‑1 (MQ=255)                                                                                                                   aTCCACGACAATCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGt                     <  2:1038622/140‑1 (MQ=255)                                                                                                                              tCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTg          >  1:225206/1‑140 (MQ=255)                                                                                                                              tCCAGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTg          >  1:570060/1‑140 (MQ=255)                                                                                                                                 aGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGAt       <  2:612073/140‑1 (MQ=255)                                                                                                                                 aGTCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGAt       <  1:243416/140‑1 (MQ=255)                                                                                                                                   tCGGCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGAttt     >  2:665496/1‑140 (MQ=255)                                                                                                                                     ggCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGATTTGc   <  2:723684/140‑1 (MQ=255)                                                                                                                                     ggCGCCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGATTTGc   <  2:731738/140‑1 (MQ=255)                                                                                                                                      gcgcCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGATTTGcc  <  2:570060/140‑1 (MQ=255)                                                                                                                                      gcgcCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGATTTGcc  <  1:322878/140‑1 (MQ=255)                                                                                                                                      gcgcCGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGATTTGcc  <  2:225206/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGGACATAACATGACGGCTGGACGTACCCGTACAGATGATCATGCAGTCGGTGATGCTGGATTTGCC  >  NC_000913/668680‑668952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑