Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	809,220	A→G	I13T (ATC→ACC)	bioA ←	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	809,220	0	A	G	100.0%	147.7 / NA	41	I13T (ATC→ACC)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/24);  total (17/24)

TGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACC  >  NC_000913/809088‑809353                                                                                                                                     |                                                                                                                                      gggATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCg                                                                                                                                <  2:677748/139‑1 (MQ=255)   gATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGtt                                                                                                                              >  2:707671/1‑140 (MQ=255)   gATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGtt                                                                                                                              >  2:397291/1‑140 (MQ=255)      cgccgcCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGt                                                                                                                           >  1:1045290/1‑140 (MQ=255)       gccgccCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTc                                                                                                                          <  1:190405/140‑1 (MQ=255)       gccgccCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTc                                                                                                                          <  1:397291/140‑1 (MQ=255)       gccgccCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTc                                                                                                                          <  2:1144218/140‑1 (MQ=255)       gccgccCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTc                                                                                                                          <  1:707671/140‑1 (MQ=255)           cccACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAg                                                                                                                      <  2:88489/140‑1 (MQ=255)                   gacgacATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATc                                                                                                              >  1:69154/1‑140 (MQ=255)                        cATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCg                                                                                                         <  1:249196/140‑1 (MQ=255)                             cGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTc                                                                                                    >  2:792631/1‑140 (MQ=255)                                 aaCCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCATCCGTTGTCATaa                                                                                                <  2:1045290/140‑1 (MQ=255)                                  aCCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGt                                                                                                        >  1:315035/1‑131 (MQ=255)                                  aCCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGt                                                                                                        <  2:315035/131‑1 (MQ=255)                                  aCCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAAt                                                                                               >  1:991877/1‑140 (MQ=255)                                       gCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACt                                                                                          >  2:44625/1‑140 (MQ=255)                                           cTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTa                                                                                      >  1:982296/1‑140 (MQ=255)                                            tGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTaa                                                                                     >  2:213017/1‑140 (MQ=255)                                                      cAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGa                                                                           <  1:792631/140‑1 (MQ=255)                                                      cAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGa                                                                           <  2:991877/140‑1 (MQ=255)                                                      cAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGa                                                                           <  2:876653/140‑1 (MQ=255)                                                        aaaTCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATAGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGaaa                                                                         <  1:1204981/140‑1 (MQ=255)                                                              gCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGCTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGAttt                                                                   <  2:982296/140‑1 (MQ=255)                                                               cTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTa                                                                  >  2:311733/1‑140 (MQ=255)                                                               cTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTa                                                                  >  2:600188/1‑140 (MQ=255)                                                                     aCCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTa                                                            <  2:910276/140‑1 (MQ=255)                                                                     aCCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTa                                                            <  1:311733/140‑1 (MQ=255)                                                                      ccTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTAc                                                           <  1:547724/140‑1 (MQ=255)                                                                              gcTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTac                                                   <  1:102825/140‑1 (MQ=255)                                                                              gcTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTATAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTac                                                   <  2:69154/140‑1 (MQ=255)                                                                                tCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTacac                                                 >  2:422859/1‑140 (MQ=255)                                                                                           tAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTaacaa                                      <  1:213017/140‑1 (MQ=255)                                                                                                        gggAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGtt                         <  1:600188/140‑1 (MQ=255)                                                                                                        gggAGGTCATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGtt                         <  2:40639/140‑1 (MQ=255)                                                                                                               cATGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGgaga                  <  1:44625/140‑1 (MQ=255)                                                                                                                aTGGATGTGTATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGaa                 >  1:858009/1‑140 (MQ=255)                                                                                                                       tgtATGGGTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCAt          >  1:70266/1‑140 (MQ=255)                                                                                                                          aTGGGTGCCAGGCATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGCAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGc       >  2:110230/1‑140 (MQ=255)                                                                                                                            gggTGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTc     <  1:23842/140‑1 (MQ=255)                                                                                                                               tGCCAGGTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCAcc  >  1:720294/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      TGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACC  >  NC_000913/809088‑809353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑