Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	958,726	A→G	E358E (GAA→GAG)	serC →	phosphoserine/phosphohydroxythreonine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	958,726	0	A	G	100.0%	274.1 / NA	76	E358E (GAA→GAG)	serC	phosphoserine/phosphohydroxythreonine aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/31);  total (45/31)

TGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTA  >  NC_000913/958594‑958860                                                                                                                                     |                                                                                                                                       tGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTc                                                                                                                                 >  2:273300/1‑140 (MQ=255)      agTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGt                                                                                                                            >  2:708339/1‑140 (MQ=255)         cTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTaa                                                                                                                         >  1:764071/1‑140 (MQ=255)                  ctgGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAAt                                                                                                                >  1:1061147/1‑140 (MQ=255)                  ctgGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAAt                                                                                                                >  1:1052583/1‑140 (MQ=255)                  ctgGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAAt                                                                                                                >  2:735813/1‑140 (MQ=255)                               aCTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATccc                                                                                                   <  1:679336/140‑1 (MQ=255)                                 tGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCca                                                                                                 >  2:604253/1‑140 (MQ=255)                                   aaaGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCcaca                                                                                               >  1:16616/1‑140 (MQ=255)                                    aaGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCAcag                                                                                              <  2:16616/140‑1 (MQ=255)                                      ggTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCAcagcc                                                                                            <  2:764071/140‑1 (MQ=255)                                       gTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCAcagcca                                                                                           >  1:125356/1‑140 (MQ=255)                                          aCCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACagccagcc                                                                                        >  2:248675/1‑140 (MQ=255)                                           ccGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTaa                                                                                                       >  1:716238/1‑124 (MQ=255)                                           ccGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTaa                                                                                                       <  2:716238/124‑1 (MQ=255)                                           ccGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCt                                                                                       >  1:922025/1‑140 (MQ=255)                                              tgtgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATcccc                                                                                                  >  1:269637/1‑126 (MQ=255)                                              tgtgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATcccc                                                                                                  <  2:269637/126‑1 (MQ=255)                                                tgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTggg                                                                                  <  2:1061147/140‑1 (MQ=255)                                                tgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTggg                                                                                  <  2:1052583/140‑1 (MQ=255)                                                 ggTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTCAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTgggg                                                                                 >  2:717126/1‑140 (MQ=255)                                                      gcggAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGttttt                                                                            >  2:537003/1‑140 (MQ=255)                                                      gcggAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTAACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGttttt                                                                            >  2:765376/1‑140 (MQ=255)                                                        ggAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAt                                                                          <  2:251973/140‑1 (MQ=255)                                                          aaTGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAttt                                                                        >  2:90415/1‑140 (MQ=255)                                                            tGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAttt                                                                        >  2:1096413/1‑138 (MQ=255)                                                            tGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAttt                                                                        <  1:1096413/138‑1 (MQ=255)                                                              cgcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgt                                                                    >  1:729306/1‑140 (MQ=255)                                                               gcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgtt                                                                   <  2:125356/140‑1 (MQ=255)                                                               gcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgtt                                                                   <  1:735813/140‑1 (MQ=255)                                                                cgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgttg                                                                  >  1:1233839/1‑140 (MQ=255)                                                                  cTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTa                                                                <  2:416837/140‑1 (MQ=255)                                                                         ttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagtt                                                         <  1:248675/140‑1 (MQ=255)                                                                             aaCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgagt                                                     >  2:410450/1‑140 (MQ=255)                                                                             aaCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgagt                                                     >  1:978792/1‑140 (MQ=255)                                                                             aaCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgagt                                                     >  1:530607/1‑140 (MQ=255)                                                                             aaCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgagt                                                     >  1:1148982/1‑140 (MQ=255)                                                                             aaCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgagt                                                     >  1:1070347/1‑140 (MQ=255)                                                                               cGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTc                                                   >  2:414560/1‑140 (MQ=255)                                                                               cGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTc                                                   >  2:691912/1‑140 (MQ=255)                                                                               cGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTc                                                   >  1:456320/1‑140 (MQ=255)                                                                               cGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTc                                                   >  2:1226905/1‑140 (MQ=255)                                                                               cGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAACCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTc                                                   >  1:1084627/1‑140 (MQ=255)                                                                                  cATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATg                                                >  1:158707/1‑140 (MQ=255)                                                                                    tGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGa                                              >  1:360847/1‑140 (MQ=255)                                                                                    tGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGa                                              >  1:952271/1‑140 (MQ=255)                                                                                         cTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATccc                                         >  1:817993/1‑140 (MQ=255)                                                                                         cTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATccc                                         >  2:716865/1‑140 (MQ=255)                                                                                          tGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCt                                        >  1:559967/1‑140 (MQ=255)                                                                                          tGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCt                                        <  2:817993/140‑1 (MQ=255)                                                                                          tGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCt                                        >  2:1062634/1‑140 (MQ=255)                                                                                           ggAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTg                                       <  1:604253/140‑1 (MQ=255)                                                                                           ggAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTg                                       <  1:1062634/140‑1 (MQ=255)                                                                                           ggAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAACTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTg                                       <  2:1070347/140‑1 (MQ=255)                                                                                                gCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGtt                                  >  2:301146/1‑140 (MQ=255)                                                                                                 cGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTa                                 <  2:530607/140‑1 (MQ=255)                                                                                                  gTTAAAGCGCTGATAGACCTCATGGTTGAGTTCGAACGACGTCACGTTAAATGCCGAAATTTTGATTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTAc                                <  2:729306/140‑1 (MQ=255)                                                                                                  gTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTAc                                <  2:922025/140‑1 (MQ=255)                                                                                                  gTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTCAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCTTGACGTTAc                                <  1:717126/140‑1 (MQ=255)                                                                                                         cgcTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCAt                         <  1:765376/140‑1 (MQ=255)                                                                                                          gcTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATc                        >  1:332903/1‑140 (MQ=255)                                                                                                           cTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAAcc                            >  2:335314/1‑135 (MQ=255)                                                                                                           cTGACAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAAcc                            <  1:335314/135‑1 (MQ=255)                                                                                                              aCAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTc                    >  1:380970/1‑140 (MQ=255)                                                                                                              aCAGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTc                    <  2:380970/140‑1 (MQ=255)                                                                                                                aGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCgt                  <  1:414560/140‑1 (MQ=255)                                                                                                                aGACTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCgt                  <  1:537003/140‑1 (MQ=255)                                                                                                                   cTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTc               <  2:332903/140‑1 (MQ=255)                                                                                                                   cTTCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTc               >  2:128362/1‑140 (MQ=255)                                                                                                                     tCATTGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGa             <  2:1133760/140‑1 (MQ=255)                                                                                                                     tCATGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGa             <  2:978792/140‑1 (MQ=255)                                                                                                                       aTGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATAGCTCGGGTCGATg           >  1:353531/1‑140 (MQ=255)                                                                                                                        tGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATgg          <  1:90415/140‑1 (MQ=255)                                                                                                                        tGGTTGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAACCAGCCTGTGGGGTTTTTCTTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATgg          <  2:1084627/140‑1 (MQ=255)                                                                                                                            tGAGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACt      <  1:716865/140‑1 (MQ=255)                                                                                                                              aGTTCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTAt    >  1:281734/1‑140 (MQ=255)                                                                                                                                ttCGAGCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTa  >  1:555864/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       TGAAGAGTCTTTTGCTGCTGGCCTTCATGCACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTA  >  NC_000913/958594‑958860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑