Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,761	G→A	G311S (GGT→AGT)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,761	0	G	A	100.0%	195.6 / NA	55	G311S (GGT→AGT)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/29);  total (26/29)

GACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGA  >  NC_000913/1336626‑1336894                                                                                                                                        |                                                                                                                                      gACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGc                                                                                                                                   <  1:117427/140‑1 (MQ=255)        ttCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCtgt                                                                                                                            <  2:150794/140‑1 (MQ=255)           tcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGc                                                                                                                         <  1:1075184/140‑1 (MQ=255)             aCTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGctt                                                                                                                       >  2:96866/1‑140 (MQ=255)             aCTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGctt                                                                                                                       <  1:1174433/140‑1 (MQ=255)             aCTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGctt                                                                                                                       <  1:795928/140‑1 (MQ=255)                      cAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAAt                                                                                                              <  2:663065/140‑1 (MQ=255)                      cAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAAt                                                                                                              <  1:987119/140‑1 (MQ=255)                              gcgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTg                                                                                                      >  2:217502/1‑140 (MQ=255)                              gcgcAAACATGGCGTGGTAGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTg                                                                                                      >  2:495012/1‑140 (MQ=255)                               cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATc                                                                                                             >  2:685044/1‑132 (MQ=255)                               cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATc                                                                                                             <  1:685044/132‑1 (MQ=255)                               cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGa                                                                                                           <  2:237262/134‑1 (MQ=255)                               cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGa                                                                                                           >  1:237262/1‑134 (MQ=255)                               cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGt                                                                                                     <  2:80408/140‑1 (MQ=255)                                   aaCATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAcc                                                                                                 <  1:217502/140‑1 (MQ=255)                                   aaCATGGCGTGGTAGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAcc                                                                                                 <  1:495012/140‑1 (MQ=255)                                     cATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCt                                                                                               <  1:475885/140‑1 (MQ=255)                                      aTGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTa                                                                                                    >  1:562283/1‑134 (MQ=255)                                      aTGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTa                                                                                                    <  2:562283/134‑1 (MQ=255)                                            tggtggGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTAc                                                                                        <  1:629387/140‑1 (MQ=255)                                              gtggGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACAt                                                                                      >  1:780280/1‑140 (MQ=255)                                              gtggGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACAt                                                                                      >  2:243328/1‑140 (MQ=255)                                                         cgtcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCg                                                                           >  2:707888/1‑140 (MQ=255)                                                           tcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCggg                                                                         <  1:243328/140‑1 (MQ=255)                                                                     tGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAg                                                               >  2:1187072/1‑140 (MQ=255)                                                                      ggTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGa                                                              <  1:96866/140‑1 (MQ=255)                                                                      ggTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGa                                                              <  1:1187072/140‑1 (MQ=255)                                                                          aTGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGc                                                                   <  2:509743/131‑1 (MQ=255)                                                                          aTGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGc                                                                   >  1:509743/1‑131 (MQ=255)                                                                          aTGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCg                                                                  >  2:1028305/1‑132 (MQ=255)                                                                          aTGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCg                                                                  <  1:1028305/132‑1 (MQ=255)                                                                          aTGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAg                                                          >  1:871752/1‑140 (MQ=255)                                                                              tCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCg                                                      >  2:1232461/1‑140 (MQ=255)                                                                                 ggATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGt                                                   <  2:1015153/140‑1 (MQ=255)                                                                                 ggATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGt                                                   <  1:707888/140‑1 (MQ=255)                                                                                        cTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCg                                             >  2:1147579/1‑139 (MQ=255)                                                                                        cTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCg                                             <  1:1147579/139‑1 (MQ=255)                                                                                        cTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGa                                            >  1:687590/1‑140 (MQ=255)                                                                                         tACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGa                                            <  1:463325/139‑1 (MQ=255)                                                                                         tACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGa                                            >  2:463325/1‑139 (MQ=255)                                                                                            cGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGaaaaa                                        >  2:174894/1‑140 (MQ=255)                                                                                              ttGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAata                                      >  2:679984/1‑140 (MQ=255)                                                                                                ggCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATg                                    >  2:978257/1‑140 (MQ=255)                                                                                                ggCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATg                                    >  2:1067483/1‑140 (MQ=255)                                                                                                   ggATCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCa                                 <  2:871752/140‑1 (MQ=255)                                                                                                      tCGCGCCACCATTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAg                              >  2:1142186/1‑140 (MQ=255)                                                                                                                aTTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCAt                    <  2:687590/140‑1 (MQ=255)                                                                                                                aTTGCCAATATGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCAt                    <  1:1142186/140‑1 (MQ=255)                                                                                                                        tatGTCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTa            >  2:1024082/1‑140 (MQ=255)                                                                                                                            tCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGc                      <  2:661594/126‑1 (MQ=255)                                                                                                                            tCGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGc                      >  1:661594/1‑126 (MQ=255)                                                                                                                             cGCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCg       >  1:213443/1‑140 (MQ=255)                                                                                                                              gCCAGAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCgg      >  2:1054772/1‑140 (MQ=255)                                                                                                                                  gAATATAGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGa  <  1:1044459/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGA  >  NC_000913/1336626‑1336894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑