Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,393,439	T→C	N71N (AAT→AAC)	mppA →	murein tripeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,393,439	0	T	C	100.0%	225.2 / NA	62	N71N (AAT→AAC)	mppA	murein tripeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/35);  total (27/35)

CCGAGCGGCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAATCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGGCGCAAGATTTTGTCT  >  NC_000913/1393302‑1393575                                                                                                                                          |                                                                                                                                         ccGAGCGGCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCa                                                                                                                                        >  1:21985/1‑140 (MQ=255)      cGGCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACg                                                                                                                                   <  1:711364/140‑1 (MQ=255)       ggCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGa                                                                                                                                  <  2:636796/140‑1 (MQ=255)               aCTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAgggg                                                                                                                          <  1:88639/140‑1 (MQ=255)                  ggCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGgaga                                                                                                                       <  1:261071/140‑1 (MQ=255)                   gCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGAt                                                                                                                      >  2:31935/1‑140 (MQ=255)                      gagaAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGt                                                                                                                   >  2:346409/1‑140 (MQ=255)                        gaAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCGGAACGAAAAAGGGGAGATTGTcc                                                                                                                 >  1:334162/1‑140 (MQ=255)                        gaAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTcc                                                                                                                 >  1:1050290/1‑140 (MQ=255)                        gaAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTcc                                                                                                                 >  2:261170/1‑140 (MQ=255)                        gaAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTcc                                                                                                                 >  2:1017457/1‑140 (MQ=255)                            cAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCgg                                                                                                             <  1:534489/140‑1 (MQ=255)                                 gCTGGAGCGCCACATTAAAGAAAAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGCCCTGCCAGAGCTTCATGTCATTCGCGATCTGTTTGAGCGTCTGGTGAACCAGAACGACAAAGGGGAGACTGTCCCCgacactt                                                                                                        >  2:811701/1‑134 (MQ=255)                                    ggTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGa                                                                                                     <  1:80805/140‑1 (MQ=255)                                      tGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACt                                                                                                   <  2:952244/140‑1 (MQ=255)                                      tGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACt                                                                                                   <  1:826473/140‑1 (MQ=255)                                          ccACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAg                                                                                                >  2:546481/1‑139 (MQ=255)                                          ccACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAg                                                                                                <  1:546481/139‑1 (MQ=255)                                          ccACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGt                                                                                               >  1:1161341/1‑140 (MQ=255)                                             caTTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGa                                                                                            <  1:965231/140‑1 (MQ=255)                                                     aTGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAAt                                                                                    <  1:361485/140‑1 (MQ=255)                                                      tGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATg                                                                                   <  2:508357/140‑1 (MQ=255)                                                      tGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATg                                                                                   <  2:1050290/140‑1 (MQ=255)                                                       gAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGaaaa                                                                                         >  2:660999/1‑133 (MQ=255)                                                       gAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGaaaa                                                                                         <  1:660999/133‑1 (MQ=255)                                                        aGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGAc                                                                                 >  1:57125/1‑140 (MQ=255)                                                        aGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGAc                                                                                 >  2:525058/1‑140 (MQ=255)                                                        aGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGAc                                                                                 >  1:133418/1‑140 (MQ=255)                                                        aGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGAc                                                                                 >  1:38553/1‑140 (MQ=255)                                                          ccTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACaa                                                                               >  1:91356/1‑140 (MQ=255)                                                               gTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTa                                                                          >  1:399308/1‑140 (MQ=255)                                                                tCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTAt                                                                         <  2:765968/140‑1 (MQ=255)                                                                 cGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATc                                                                        <  2:811036/140‑1 (MQ=255)                                                                   cTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTg                                                                      <  2:1156885/140‑1 (MQ=255)                                                                   cTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTg                                                                      <  2:1161341/140‑1 (MQ=255)                                                                   cTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTg                                                                      <  2:57125/140‑1 (MQ=255)                                                                   cTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTg                                                                      <  1:261170/140‑1 (MQ=255)                                                                   cTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTg                                                                      <  2:218968/140‑1 (MQ=255)                                                                   cTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTg                                                                      <  2:21985/140‑1 (MQ=255)                                                                   cTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTg                                                                      <  1:847336/140‑1 (MQ=255)                                                                       aTCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACt                                                                  >  1:282863/1‑140 (MQ=255)                                                                           cGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAACGACAACCGTATCTGGACTTTTa                                                              >  2:895703/1‑140 (MQ=255)                                                                           cGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGCTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTa                                                              <  2:242929/140‑1 (MQ=255)                                                                             cTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTAcc                                                            <  2:133418/140‑1 (MQ=255)                                                                                  gCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTgcg                                                       <  1:1017457/140‑1 (MQ=255)                                                                                  gCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTgcg                                                       >  2:567261/1‑140 (MQ=255)                                                                                        ggCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATaa                                                 >  2:178023/1‑140 (MQ=255)                                                                                               cAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCaaaa                                          <  1:869631/140‑1 (MQ=255)                                                                                                agagATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAAt                                         <  1:246993/140‑1 (MQ=255)                                                                                                    aTTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGc                                     <  1:525058/140‑1 (MQ=255)                                                                                                           tCATTCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGc                              <  2:38553/140‑1 (MQ=255)                                                                                                             aTTCGCGATCTGTTTGAAGGTCTGGTGAACCGGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGcac                            <  2:334162/140‑1 (MQ=255)                                                                                                              ttCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGcaca                           >  1:562619/1‑140 (MQ=255)                                                                                                               tCGCGATCTGTTTGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCacac                          <  2:91356/140‑1 (MQ=255)                                                                                                                         tttGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGcac                            <  2:41447/128‑1 (MQ=255)                                                                                                                         tttGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGcac                            >  1:41447/1‑128 (MQ=255)                                                                                                                         tttGAAGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGgc                >  1:188947/1‑140 (MQ=255)                                                                                                                              aGGTCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGGCGCAAg           <  2:562619/140‑1 (MQ=255)                                                                                                                                 tCTGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGGCGCAAGAtt        >  2:470940/1‑140 (MQ=255)                                                                                                                                   tGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGGCGCAAGAtttt      >  1:569224/1‑140 (MQ=255)                                                                                                                                   tGGTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGGCGCAAGAtttt      >  2:915352/1‑140 (MQ=255)                                                                                                                                     gTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGGCGCAAGATTTTGt    >  2:1047832/1‑140 (MQ=255)                                                                                                                                     gTGAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGGCGCAAGATTTTGt    <  1:31935/140‑1 (MQ=255)                                                                                                                                       gAACCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGGCGCAAGATTTTGTCt  <  1:567261/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CCGAGCGGCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAATCAGAACGAAAAAGGGGAGATTGTCCCCGGCGTTGCGACTCAGTGGAAAAGTAATGACAACCGTATCTGGACTTTTACCCTGCGCGATAACGCAAAATGGGCGGATGGCACACCGGTAACGGCGCAAGATTTTGTCT  >  NC_000913/1393302‑1393575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑