Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	83,945	A→G	intergenic (‑237/‑423)	leuL ← / → leuO	leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	83,945	0	A	G	100.0%	255.4 / NA	71	intergenic (‑237/‑423)	leuL/leuO	leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/41);  total (30/41)

GCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGACAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATA  >  NC_000913/83810‑84075                                                                                                                                        |                                                                                                                                    gCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCaaa                                                                                                                                 >  2:394068/1‑140 (MQ=255) gCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCaaa                                                                                                                                 >  1:605244/1‑140 (MQ=255)  cATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCaaaa                                                                                                                                >  2:1002510/1‑140 (MQ=255)  cATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCaaaa                                                                                                                                >  1:744184/1‑140 (MQ=255)       gTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCAc                                                                                                                           >  1:382124/1‑140 (MQ=255)        tAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCAcg                                                                                                                          <  1:954255/140‑1 (MQ=255)         aaaaTTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCAcgc                                                                                                                         <  1:1201782/140‑1 (MQ=255)         aaaaTTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCAcgc                                                                                                                         <  1:568691/140‑1 (MQ=255)          aaaTTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCAcgcg                                                                                                                        <  2:299772/140‑1 (MQ=255)              tAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGc                                                                                                                    <  1:1078439/140‑1 (MQ=255)              tAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGc                                                                                                                    <  2:182949/140‑1 (MQ=255)              tAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGc                                                                                                                    <  1:126380/140‑1 (MQ=255)              tAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGc                                                                                                                    <  2:382124/140‑1 (MQ=255)                gCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCaa                                                                                                                  >  2:447653/1‑140 (MQ=255)                 cTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCaaa                                                                                                                 >  1:1132946/1‑140 (MQ=255)                    aTTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAAcc                                                                                                              <  1:225554/140‑1 (MQ=255)                     ttGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCt                                                                                                             >  2:353271/1‑140 (MQ=255)                      tgtgCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTa                                                                                                            <  1:63511/140‑1 (MQ=255)                        tgctgcGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATc                                                                                                          <  2:1132946/140‑1 (MQ=255)                          ctgcGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGc                                                                                                                    <  1:264964/128‑1 (MQ=255)                          ctgcGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGc                                                                                                                    >  2:264964/1‑128 (MQ=255)                          ctgcGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCt                                                                                                        >  2:708995/1‑140 (MQ=255)                           tgcGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCtg                                                                                                       >  1:817813/1‑140 (MQ=255)                              ggtggtTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCtgatg                                                                                                    >  1:647996/1‑140 (MQ=255)                                  gtTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAGAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGAttt                                                                                                >  1:896859/1‑140 (MQ=255)                                  gtTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAAcc                                                                                                              <  2:126184/126‑1 (MQ=255)                                  gtTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAAcc                                                                                                              >  1:126184/1‑126 (MQ=255)                                  gtTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGAttt                                                                                                >  2:107533/1‑140 (MQ=255)                                  gtTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGAttt                                                                                                <  1:882297/140‑1 (MQ=255)                                       aGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTAtt                                                                                           <  1:394068/140‑1 (MQ=255)                                       aGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTAtt                                                                                           >  1:325330/1‑140 (MQ=255)                                       aGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTAtt                                                                                           >  2:1169795/1‑140 (MQ=255)                                       aGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTAtt                                                                                           <  2:479348/140‑1 (MQ=255)                                       aGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTAtt                                                                                           <  2:647996/140‑1 (MQ=255)                                                        tCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCg                                                                          <  2:576428/140‑1 (MQ=255)                                                          tGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑t                                                                       >  2:403978/1‑140 (MQ=255)                                                          tGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑t                                                                       >  2:1233005/1‑140 (MQ=255)                                                          tGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑t                                                                       >  1:1029887/1‑140 (MQ=255)                                                          tGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑t                                                                       >  1:288162/1‑140 (MQ=255)                                                            cTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑ttt                                                                     >  2:624359/1‑140 (MQ=255)                                                            cTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑ttt                                                                     >  1:47532/1‑140 (MQ=255)                                                               aaCTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑tttttt                                                                  >  2:48604/1‑140 (MQ=255)                                                                aCTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑ttttttt                                                                 <  2:605244/140‑1 (MQ=255)                                                                     cGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGata                                                            <  1:48604/140‑1 (MQ=255)                                                                      gACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGatat                                                           >  2:777771/1‑140 (MQ=255)                                                                         gCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑‑TTTTTTTGATATTGAt                                                       <  2:47532/140‑1 (MQ=255)                                                                          cTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGAt                                                       <  1:1169795/140‑1 (MQ=255)                                                                          cTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGAt                                                       <  2:230037/140‑1 (MQ=255)                                                                             ttCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTg                                                    <  1:708995/140‑1 (MQ=255)                                                                             ttCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTg                                                    <  2:460249/140‑1 (MQ=255)                                                                              tCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTgg                                                   >  2:257357/1‑140 (MQ=255)                                                                               cATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGt                                                  <  2:1029887/140‑1 (MQ=255)                                                                                aTCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGATTTTTTTTTGATATTGATTTGGt                                                  <  1:618028/140‑1 (MQ=255)                                                                                  cGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGaa                                               <  2:222344/140‑1 (MQ=255)                                                                                  cGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGaa                                               <  1:624359/140‑1 (MQ=255)                                                                                      tGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAAtatt                                           <  1:107533/140‑1 (MQ=255)                                                                                       gACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAAtatta                                          >  1:301063/1‑140 (MQ=255)                                                                                           tatTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAGAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGa                                      <  2:896859/140‑1 (MQ=255)                                                                                           tatTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGa                                      <  1:257357/140‑1 (MQ=255)                                                                                                  gCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATc                                    >  2:567367/1‑135 (MQ=255)                                                                                                  gCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATc                                    <  1:567367/135‑1 (MQ=255)                                                                                                   cATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTaa                              >  1:1073160/1‑140 (MQ=255)                                                                                                       aaTTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGtt                          <  1:287926/140‑1 (MQ=255)                                                                                                       aaTTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGtt                          <  2:1073160/140‑1 (MQ=255)                                                                                                        aTTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGtt                          >  1:767618/1‑139 (MQ=255)                                                                                                        aTTTTTGCATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTGTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGtt                          <  2:767618/139‑1 (MQ=255)                                                                                                               cATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTa                  <  1:1002510/140‑1 (MQ=255)                                                                                                               cATCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTa                  <  1:353271/140‑1 (MQ=255)                                                                                                                aTCTAATCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTaa                 <  1:777771/140‑1 (MQ=255)                                                                                                                      tCAACGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCAtt           <  2:288162/140‑1 (MQ=255)                                                                                                                         aCGAGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTaaa        <  2:749317/140‑1 (MQ=255)                                                                                                                            aGGAAAAAGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAatat     >  2:661489/1‑140 (MQ=255)                                                                                                                               aaaaaGGGGGCAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAAtatata  >  1:750631/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    GCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTACCGACGCTTTTCATCGGTTGACATATTTCAGCATAAATTTTTGCATCTAATCAACGAGGAAAAAGGGGACAAAATGCACGCGTTGCAAAACCTATCCTGATGATTTGTATTGAATTATATGTTTTGCGA‑TTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATA  >  NC_000913/83810‑84075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑