Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,466,858	T→C	pseudogene (1467/2518 nt)	ydbA →	putative outer membrane protein, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,466,858	0	T	C	100.0%	212.8 / NA	59	pseudogene (1467/2518 nt)	ydbA	putative outer membrane protein, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

ATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCA  >  NC_000913/1466775‑1466991                                                                                    |                                                                                                                                      aTTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATggcgg                                                                               <  1:107671/140‑1 (MQ=255) aTTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATggcgg                                                                               <  1:393055/140‑1 (MQ=255) aTTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATggcgg                                                                               <  1:355574/140‑1 (MQ=255) aTTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATggcgg                                                                               <  1:330418/140‑1 (MQ=255)    gATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATTGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGcggcac                                                                            <  2:617385/140‑1 (MQ=255)    gATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGcggcac                                                                            <  2:847017/140‑1 (MQ=255)          aCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGcggcaccggcac                                                                      >  2:110241/1‑140 (MQ=255)           ccGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACt                                                                     <  1:541911/140‑1 (MQ=255)            cggtgACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTc                                                                    >  2:221519/5‑140 (MQ=11)            cGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTc                                                                    >  2:293443/1‑140 (MQ=255)            cGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTc                                                                    >  1:564526/1‑140 (MQ=255)              gtgACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAg                                                                  >  1:699474/3‑140 (MQ=255)               tgACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATggcgg                                                                               <  1:822633/125‑1 (MQ=255)               tgACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATggcgg                                                                               >  2:822633/2‑126 (MQ=255)               tgACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGa                                                                 >  1:294248/2‑140 (MQ=255)                gACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGAt                                                                <  2:699474/140‑1 (MQ=255)                 aCAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATggcgg                                                                               >  1:72915/1‑124 (MQ=255)                 aCAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATggcgg                                                                               <  2:72915/124‑1 (MQ=255)                   aGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCaa                                                             >  1:954641/1‑140 (MQ=255)                     cgcgaCGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACg                                                           >  2:597765/5‑140 (MQ=255)                      gcgaCGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACgg                                                          >  2:18293/4‑140 (MQ=255)                           ggTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAacg                                                     <  1:110241/140‑1 (MQ=255)                               gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGc                                                 >  1:88189/1‑140 (MQ=255)                                 tAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCa                                               <  1:221519/140‑1 (MQ=255)                                 tAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCa                                               <  2:229963/140‑1 (MQ=255)                                 tAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCa                                               <  2:88189/140‑1 (MQ=255)                                      aGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACcgcg                                          <  2:1066990/140‑1 (MQ=255)                                       gggCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGa                                         >  1:132257/1‑140 (MQ=255)                                       gggCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGa                                         >  2:1179216/1‑140 (MQ=255)                                       gggCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGa                                         >  2:1215457/1‑140 (MQ=255)                                        ggCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGaa                                        <  2:283464/140‑1 (MQ=255)                                         gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGGCACCGCGAAt                                       >  1:845500/1‑140 (MQ=255)                                         gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAAt                                       <  1:838272/140‑1 (MQ=255)                                         gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAAt                                       >  1:331491/1‑140 (MQ=255)                                         gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAAt                                       <  2:845500/140‑1 (MQ=255)                                             cATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACa                                   <  1:682937/140‑1 (MQ=255)                                             cATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACa                                   <  1:230999/140‑1 (MQ=255)                                               tGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGt                                 <  1:18293/140‑1 (MQ=255)                                                  ccGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGa                              <  1:1215457/140‑1 (MQ=255)                                                   cGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCa                                               <  2:551113/122‑1 (MQ=255)                                                   cGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCa                                               >  1:551113/1‑122 (MQ=255)                                                       aCCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAAc                         >  1:3777/1‑140 (MQ=255)                                                       aCCGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAAc                         >  1:416070/1‑140 (MQ=255)                                                         cGATCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCa                       <  2:132257/140‑1 (MQ=255)                                                           aTCCGGAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACt                     >  2:935988/1‑140 (MQ=255)                                                               ggAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTg                 <  1:293443/140‑1 (MQ=255)                                                                gAGTCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGa                >  2:600482/1‑140 (MQ=255)                                                                   tCCATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATgg             >  2:1072021/1‑140 (MQ=255)                                                                     catgggTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGaa           >  2:513980/5‑140 (MQ=255)                                                                     catgggTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGaa           >  2:974272/5‑140 (MQ=255)                                                                     cATGGGTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGaa           <  2:416070/140‑1 (MQ=255)                                                                      atgggTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTg                 >  2:977752/4‑133 (MQ=255)                                                                      atgggTATCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTg                 <  1:977752/130‑1 (MQ=255)                                                                             tCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTcc   >  2:659565/1‑140 (MQ=255)                                                                             tCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTcc   >  2:158238/1‑140 (MQ=255)                                                                             tCCAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTcc   >  1:463590/1‑140 (MQ=255)                                                                              ccAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCa  <  2:463590/140‑1 (MQ=255)                                                                              ccAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCa  <  1:513980/140‑1 (MQ=255)                                                                              ccAGATCGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCa  <  1:1072021/140‑1 (MQ=255)                                                                                    |                                                                                                                                      ATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCA  >  NC_000913/1466775‑1466991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑