Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,569,333	A→G	F386S (TTT→TCT)	gadC ←	L‑glutamate:4‑aminobutyrate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,569,333	0	A	G	100.0%	280.5 / NA	77	F386S (TTT→TCT)	gadC	L‑glutamate:4‑aminobutyrate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/44);  total (33/44)

AGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGA  >  NC_000913/1569199‑1569460                                                                                                                                       |                                                                                                                                aggaaACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGc                                                                                                                            <  1:494542/140‑1 (MQ=255)     aaCAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGa                                                                                                                        <  2:494728/140‑1 (MQ=255)          tAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATaa                                                                                                                   >  2:1069229/1‑140 (MQ=255)             aCGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGca                                                                                                                <  1:416087/140‑1 (MQ=255)              cGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGcac                                                                                                               <  1:1069229/140‑1 (MQ=255)              cGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGcac                                                                                                               <  2:608167/140‑1 (MQ=255)                  aTAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAg                                                                                                           >  2:940093/1‑140 (MQ=255)                   tAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGa                                                                                                          <  1:813334/140‑1 (MQ=255)                     aTTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATa                                                                                                        <  2:113429/140‑1 (MQ=255)                               gcagACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCaccacc                                                                                              >  1:104687/1‑140 (MQ=255)                                cagACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCg                                                                                             >  2:754917/1‑140 (MQ=255)                                cagACCGACAATTGCCACGACCAGTTTCACCCCTTTACCA‑CAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGt                                                                                            >  2:803907/1‑140 (MQ=255)                                    ccGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAg                                                                                         <  2:482809/140‑1 (MQ=255)                                        cAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATcacca                                                                                                >  2:481408/1‑129 (MQ=255)                                        cAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATcacca                                                                                                <  1:481408/129‑1 (MQ=255)                                        cAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGcc                                                                                     >  2:268286/1‑140 (MQ=255)                                           ttGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGt                                                                                  <  2:395628/140‑1 (MQ=255)                                             gCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAgcg                                                                                       <  1:326481/133‑1 (MQ=255)                                             gCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAgcg                                                                                       >  2:326481/1‑133 (MQ=255)                                             gCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGc                                                                                >  1:457500/1‑140 (MQ=255)                                              ccACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCg                                                                               <  1:13007/140‑1 (MQ=255)                                               cACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGa                                                                              >  2:110885/1‑140 (MQ=255)                                                 cGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATc                                                                            >  2:528520/1‑140 (MQ=255)                                                   aCCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGGCAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCag                                                                          <  2:921197/140‑1 (MQ=255)                                                     cAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCagga                                                                        <  1:550842/140‑1 (MQ=255)                                                          tCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGAc                                                                   <  1:714068/140‑1 (MQ=255)                                                            aCCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACAt                                                                 >  2:660043/1‑140 (MQ=255)                                                             ccccTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACAtg                                                                <  1:437031/140‑1 (MQ=255)                                                             ccccTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACAtg                                                                <  1:332042/140‑1 (MQ=255)                                                                  ttACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATgttgtt                                                           >  2:1190812/1‑140 (MQ=255)                                                                  ttACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATgttgtt                                                           >  1:953965/1‑140 (MQ=255)                                                                        ccaGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGcc                                                     <  1:940093/140‑1 (MQ=255)                                                                          aGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCCCCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTAcc                                                        <  2:451530/135‑1 (MQ=255)                                                                          aGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTAcc                                                        >  1:451530/1‑135 (MQ=255)                                                                          aGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCAc                                                   <  1:1014460/140‑1 (MQ=255)                                                                          aGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCAc                                                   <  1:179430/140‑1 (MQ=255)                                                                          aGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCAc                                                   <  1:528520/140‑1 (MQ=255)                                                                          aGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCAc                                                   <  1:804877/140‑1 (MQ=255)                                                                           gggATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCAcc                                                  >  2:544803/1‑140 (MQ=255)                                                                              atatTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGt                                               <  1:660043/140‑1 (MQ=255)                                                                               tatTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTa                                              <  1:803907/140‑1 (MQ=255)                                                                               tatTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTa                                              <  2:223537/140‑1 (MQ=255)                                                                               tatTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTa                                              <  1:353211/140‑1 (MQ=255)                                                                                atTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTAc                                                         >  2:423445/1‑128 (MQ=255)                                                                                atTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTAc                                                         <  1:423445/128‑1 (MQ=255)                                                                                     aTGTGCGTTTTAAGTCAGGATGTTTAATAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTg                                        <  2:1142489/140‑1 (MQ=255)                                                                                     aTGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTg                                        <  2:48196/140‑1 (MQ=255)                                                                                       gtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTa                                              <  2:526125/132‑1 (MQ=255)                                                                                       gtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTa                                              >  1:526125/1‑132 (MQ=255)                                                                                       gtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAg                                      >  2:1125244/1‑140 (MQ=255)                                                                                       gtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAg                                      >  2:1229953/1‑140 (MQ=255)                                                                                       gtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAg                                      >  1:1011219/1‑140 (MQ=255)                                                                                        tgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAgg                                     >  1:1043160/1‑140 (MQ=255)                                                                                        tgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAACCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAgg                                     >  2:607200/1‑140 (MQ=255)                                                                                         gCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGt                                               <  2:1019293/129‑1 (MQ=255)                                                                                         gCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGt                                               >  1:1019293/1‑129 (MQ=255)                                                                                          cGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCAc                                                   >  2:44069/1‑124 (MQ=255)                                                                                          cGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCAc                                                   <  1:44069/124‑1 (MQ=255)                                                                                          cGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGgat                                   >  1:1185881/1‑140 (MQ=255)                                                                                          cGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGgat                                   >  2:1228177/1‑140 (MQ=255)                                                                                          cGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCAAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGgat                                   >  1:548431/1‑140 (MQ=255)                                                                                             tttAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGgatgat                                <  2:104687/140‑1 (MQ=255)                                                                                                    cAGGATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAAcgcg                         <  1:754917/140‑1 (MQ=255)                                                                                                     aGGATGTTTAAGAACCAACACAATGTAGCAAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGa                        <  2:548431/140‑1 (MQ=255)                                                                                                      ggATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGAt                       <  2:767767/140‑1 (MQ=255)                                                                                                       gATGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATa                      >  1:699669/1‑140 (MQ=255)                                                                                                        aTGTTTAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAg                     <  1:1229953/140‑1 (MQ=255)                                                                                                           tttAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACg                  <  1:268286/140‑1 (MQ=255)                                                                                                           tttAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACg                  <  1:164757/140‑1 (MQ=255)                                                                                                           tttAAGAACCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACg                  <  2:699669/140‑1 (MQ=255)                                                                                                           tttAAGAACCAACACAATGTAACCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACg                  <  1:607200/140‑1 (MQ=255)                                                                                                                  aCCAACACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCAc           <  2:1011219/140‑1 (MQ=255)                                                                                                                     aaCACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAg        >  1:531067/1‑140 (MQ=255)                                                                                                                     aaCACAATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAg        >  1:590959/1‑140 (MQ=255)                                                                                                                       cacaATGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCt      <  2:934640/140‑1 (MQ=255)                                                                                                                           aTGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGa  >  2:151169/1‑140 (MQ=255)                                                                                                                           aTGTAGCCAATAGACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGa  >  2:1004461/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                AGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGA  >  NC_000913/1569199‑1569460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑