Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,868,482	G→A	G525G (GGG→GGA)	yeaG →	protein kinase YeaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,868,482	0	G	A	100.0%	198.1 / NA	55	G525G (GGG→GGA)	yeaG	protein kinase YeaG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/27);  total (28/27)

TTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGCGGACG  >  NC_000913/1868351‑1868616                                                                                                                                    |                                                                                                                                       tttATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGtt                                                                                                                                >  2:446487/1‑140 (MQ=255)    aTCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGa                                                                                                                             >  2:982851/1‑140 (MQ=255)           aaGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAg                                                                                                                      <  1:446487/140‑1 (MQ=255)              agaTCCAGACGGC‑‑ACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTctct                                                                                                                 <  1:1161556/140‑1 (MQ=255)              agaTCCAGACGGC‑‑ACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTctct                                                                                                                 <  1:949713/140‑1 (MQ=255)                     gACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACg                                                                                                            <  1:735423/140‑1 (MQ=255)                        ggCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCg                                                                                                         >  2:799188/1‑140 (MQ=255)                          ccTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAg                                                                                                       >  2:170545/1‑140 (MQ=255)                          ccTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAg                                                                                                       >  1:833240/1‑140 (MQ=255)                                         aTTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCgag                                                                                        >  1:1080421/1‑140 (MQ=255)                                         aTTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCgag                                                                                        >  1:775244/1‑140 (MQ=255)                                                  atGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACcggcg                                                                               <  1:292776/140‑1 (MQ=255)                                                  atGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACcggcg                                                                               <  1:170545/140‑1 (MQ=255)                                                   tGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACcggcgg                                                                              <  2:1224454/140‑1 (MQ=255)                                                    gggCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCggg                                                                             >  2:556590/1‑140 (MQ=255)                                                     ggCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATTAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCgggg                                                                            <  1:17947/140‑1 (MQ=255)                                                     ggCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACcggcg                                                                               >  2:1093480/1‑137 (MQ=255)                                                     ggCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACcggcg                                                                               <  1:1093480/137‑1 (MQ=255)                                                      gCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGa                                                                           >  1:248347/1‑140 (MQ=255)                                                          aaCATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAg                                                                       <  1:982851/140‑1 (MQ=255)                                                                  cGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCaa                                                               <  1:556590/140‑1 (MQ=255)                                                                       gTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGAt                                                          >  2:577052/1‑140 (MQ=255)                                                                          aTGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTc                                                       <  2:248347/140‑1 (MQ=255)                                                                          aTGTTACCTACGCGCATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTc                                                       <  2:1067050/140‑1 (MQ=255)                                                                                 cTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACg                                                <  2:833240/140‑1 (MQ=255)                                                                                  tACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACga                                               >  1:89213/1‑140 (MQ=255)                                                                                  tACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACg                                                <  1:310425/139‑1 (MQ=255)                                                                                  tACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACg                                                >  2:310425/1‑139 (MQ=255)                                                                                   aCGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACgag                                              >  1:82410/1‑140 (MQ=255)                                                                                    cgcgGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACgaga                                             >  1:542054/1‑140 (MQ=255)                                                                                     gcgGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGAt                                            >  1:856629/1‑140 (MQ=255)                                                                                     gcgGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGAt                                            >  2:1000254/1‑140 (MQ=255)                                                                                     gcgGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGAt                                            >  1:1157406/1‑140 (MQ=255)                                                                                        gATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGt                                         <  2:82410/140‑1 (MQ=255)                                                                                        gATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGt                                         <  2:472357/140‑1 (MQ=255)                                                                                        gATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGt                                         <  1:1000254/140‑1 (MQ=255)                                                                                        gATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGt                                         <  1:329256/140‑1 (MQ=255)                                                                                        gATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGt                                         <  1:1199342/140‑1 (MQ=255)                                                                                        gATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGt                                         <  2:1157406/140‑1 (MQ=255)                                                                                        gATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGt                                         <  1:27077/140‑1 (MQ=255)                                                                                          tttCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTa                                       <  2:1080421/140‑1 (MQ=255)                                                                                              tGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACtt                                   >  1:525497/1‑140 (MQ=255)                                                                                                gATTCAGGATCAGGAGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCg                                 <  2:542054/140‑1 (MQ=255)                                                                                                     aggatcaggaGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGt                                         <  2:904241/127‑1 (MQ=255)                                                                                                     aggatcaggaGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGt                                         >  1:904241/1‑127 (MQ=255)                                                                                                     aggatcaggaGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTg                            >  2:681328/1‑140 (MQ=255)                                                                                                     aggatcaggaGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTg                            >  2:769021/1‑140 (MQ=255)                                                                                                     aggatcaggaGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTg                            >  1:979610/1‑140 (MQ=255)                                                                                                         tcaggaGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTgcgcg                        <  2:856629/140‑1 (MQ=255)                                                                                                             gaGTATCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCag                    >  2:38783/1‑140 (MQ=255)                                                                                                                 aTCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCg                >  2:258056/1‑140 (MQ=255)                                                                                                                  tCGCGATCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGa               >  2:962444/1‑140 (MQ=255)                                                                                                                       aTCCGGATACCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAAc          >  2:1068239/1‑140 (MQ=255)                                                                                                                               aCCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGCGGACg  >  2:196167/1‑140 (MQ=255)                                                                                                                               aCCGGACAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGCGGACg  <  2:89213/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       TTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTCCGAATATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGCGGACG  >  NC_000913/1868351‑1868616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑