Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,915	A→G	G359G (GGT→GGC)	rnd ←	RNase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,915	0	A	G	100.0%	152.8 / NA	43	G359G (GGT→GGC)	rnd	RNase D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/19);  total (24/19)

CGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACG  >  NC_000913/1886779‑1887044                                                                                                                                         |                                                                                                                                  cGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTcgcca                                                                                                                                >  2:548485/1‑140 (MQ=255)   aaGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTCCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACg                                                                                                                              <  2:243092/140‑1 (MQ=255)                 ggTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCa                                                                                                                >  1:963286/1‑140 (MQ=255)                     ttGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTc                                                                                                            <  1:1199832/140‑1 (MQ=255)                             gTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAAt                                                                                                    >  2:95156/1‑140 (MQ=255)                             gTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAAt                                                                                                    >  2:1045558/1‑140 (MQ=255)                             gTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAAt                                                                                                    >  2:60191/1‑140 (MQ=255)                             gTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAAt                                                                                                    >  2:7837/1‑140 (MQ=255)                                   tGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCt                                                                                              <  1:95156/140‑1 (MQ=255)                                     ccGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGc                                                                                            >  1:229503/1‑140 (MQ=255)                                            cGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCa                                                                                     <  1:273495/140‑1 (MQ=255)                                                       cTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGc                                                                          >  2:551836/1‑140 (MQ=255)                                                       cTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGc                                                                          <  1:551836/140‑1 (MQ=255)                                                        tGAGCGCGCCCAGTCCGGCTTCGGAAGA‑TTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCa                                                                        >  2:21723/1‑140 (MQ=255)                                                         gAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCa                                                                        >  2:1036034/1‑140 (MQ=255)                                                                    gTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAgtt                                                             <  2:963286/140‑1 (MQ=255)                                                                    gTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAgtt                                                             >  1:815957/1‑140 (MQ=255)                                                                               gAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTcagca                                                                >  1:115506/1‑126 (MQ=255)                                                                               gAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTcagca                                                                <  2:115506/126‑1 (MQ=255)                                                                               gAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGc                                                  >  2:1157174/1‑140 (MQ=255)                                                                                          tGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGc                                       >  2:260990/1‑140 (MQ=255)                                                                                            cagATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGAt                                         >  1:552436/3‑136 (MQ=255)                                                                                            cGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGAt                                         <  2:552436/136‑1 (MQ=255)                                                                                                  ttCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAAtt                               <  1:1045558/140‑1 (MQ=255)                                                                                                  ttCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAAtt                               <  1:60191/140‑1 (MQ=255)                                                                                                   tCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTc                              >  1:393623/1‑140 (MQ=255)                                                                                                     cTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCgg                            <  1:1157174/140‑1 (MQ=255)                                                                                                       gCAATAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGgcg                          <  1:7837/140‑1 (MQ=255)                                                                                                           tAAATTGTGTAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGa                      >  1:780158/1‑140 (MQ=255)                                                                                                                 gtgtAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTAt                <  1:260990/140‑1 (MQ=255)                                                                                                                  tgtAATGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATg               >  1:736103/1‑140 (MQ=255)                                                                                                                     aaTGCTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGt            >  2:614632/1‑140 (MQ=255)                                                                                                                         cTTCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCg        <  1:175714/140‑1 (MQ=255)                                                                                                                           tCCGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCt      <  2:815957/140‑1 (MQ=255)                                                                                                                            ccGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGTAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTc     >  2:722284/1‑140 (MQ=255)                                                                                                                            ccGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTc     >  2:275139/1‑140 (MQ=255)                                                                                                                            ccGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTc     >  1:88740/1‑140 (MQ=255)                                                                                                                            ccGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTc     >  2:1161154/1‑140 (MQ=255)                                                                                                                            ccGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTc     >  2:835121/1‑140 (MQ=255)                                                                                                                             cGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCa    <  2:229503/140‑1 (MQ=255)                                                                                                                             cGCCATCAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCa    <  1:1161154/140‑1 (MQ=255)                                                                                                                                   cAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACg  <  2:576805/136‑1 (MQ=255)                                                                                                                                   cAGCTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACg  >  1:576805/1‑136 (MQ=255)                                                                                                                                      cTCGCCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCt      <  1:268642/129‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  CGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACG  >  NC_000913/1886779‑1887044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑