Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,963,498	T→G	Q518P (CAG→CCG)	flhA ←	flagellar biosynthesis protein FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,963,498	0	T	G	100.0%	237.4 / NA	66	Q518P (CAG→CCG)	flhA	flagellar biosynthesis protein FlhA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/34);  total (32/34)

CGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCTGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACTGTGTACCCCTGAATCTGCGCCTG  >  NC_000913/1963365‑1963634                                                                                                                                      |                                                                                                                                         cGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGa                                                                                                                                    >  2:1062018/1‑140 (MQ=255)   ccAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTTTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGAcg                                                                                                                                  <  2:294969/140‑1 (MQ=255)   ccAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGAcg                                                                                                                                  <  1:1056407/140‑1 (MQ=255)   ccAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGAcg                                                                                                                                  <  2:586617/140‑1 (MQ=255)   ccAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGAcg                                                                                                                                  >  1:1164350/1‑140 (MQ=255)   ccAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGAcg                                                                                                                                  <  1:1062018/140‑1 (MQ=255)    cAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGAcgc                                                                                                                                 <  1:1109209/140‑1 (MQ=255)       cGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGAt                                                                                                                              >  1:564444/1‑140 (MQ=255)                    gTGCGCATATTGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTg                                                                                                                 <  1:846178/140‑1 (MQ=255)                              cgcgAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTga                                                                                                       >  1:508032/1‑140 (MQ=255)                              cgcgAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTga                                                                                                       <  1:805238/140‑1 (MQ=255)                               gcgAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTgac                                                                                                      >  1:66833/1‑140 (MQ=255)                               gcgAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTgac                                                                                                      >  1:31674/1‑140 (MQ=255)                                        tACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACa                                                                                             >  2:677244/1‑140 (MQ=255)                                           tttttCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGct                                                                                          <  2:1212505/140‑1 (MQ=255)                                                cATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCg                                                                                     <  1:1076585/140‑1 (MQ=255)                                                  tCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCg                                                                                     >  1:36805/1‑138 (MQ=255)                                                  tCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCg                                                                                     <  2:36805/138‑1 (MQ=255)                                                      ggagATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGc                                                                               <  2:1126401/140‑1 (MQ=255)                                                       gagaTTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCt                                                                              <  1:296495/140‑1 (MQ=255)                                                       gagaTTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCt                                                                              <  2:66833/140‑1 (MQ=255)                                                             ttGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTaa                                                                        <  2:739759/140‑1 (MQ=255)                                                                   caCTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGAGCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAggg                                                                  >  2:273933/1‑139 (MQ=255)                                                                           gCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGa                                                          <  2:559312/140‑1 (MQ=255)                                                                            cAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGAt                                                         <  2:564444/140‑1 (MQ=255)                                                                            cAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGAt                                                         <  2:1117123/140‑1 (MQ=255)                                                                              gtgtGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGc                                                       <  1:799353/140‑1 (MQ=255)                                                                               tgtgGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGAt                                                         <  1:612181/137‑1 (MQ=255)                                                                               tgtgGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGAt                                                         >  2:612181/1‑137 (MQ=255)                                                                               tgtgGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCg                                                      >  2:356333/1‑140 (MQ=255)                                                                               tgtgGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCg                                                      >  2:979488/1‑140 (MQ=255)                                                                                gtggtgAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGt                                                     >  2:215110/1‑140 (MQ=255)                                                                                    tgAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTGCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCt                                                 >  2:1080050/1‑140 (MQ=255)                                                                                    tgAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCt                                                 >  1:927695/1‑140 (MQ=255)                                                                                    tgAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCt                                                 >  2:504719/1‑140 (MQ=255)                                                                                        cGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGc                                                       <  1:1187180/130‑1 (MQ=255)                                                                                        cGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGc                                                       >  2:1187180/1‑130 (MQ=255)                                                                                        cGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTacca                                             >  2:609815/1‑140 (MQ=255)                                                                                        cGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTacca                                             >  1:384909/1‑140 (MQ=255)                                                                                        cGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTacca                                             <  1:215110/140‑1 (MQ=255)                                                                                         gTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGa                                                          >  1:938390/1‑126 (MQ=255)                                                                                         gTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGa                                                          <  2:938390/126‑1 (MQ=255)                                                                                         gTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTaccac                                            >  1:121912/1‑140 (MQ=255)                                                                                           gacgacGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCg                                          >  2:932815/1‑140 (MQ=255)                                                                                           gacgacGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCg                                          >  2:275093/1‑140 (MQ=255)                                                                                             cgacgCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTg                                        <  1:609815/140‑1 (MQ=255)                                                                                              gacgCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGc                                       <  2:1164350/140‑1 (MQ=255)                                                                                                cgCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTg                                     <  1:323221/140‑1 (MQ=255)                                                                                                cgCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTg                                     >  2:475409/1‑140 (MQ=255)                                                                                                cgCCAGGAACGAGATCTTCCGTCAGATTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTg                                     <  2:927695/140‑1 (MQ=255)                                                                                                       aaCGAGATCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCaa                              <  2:614237/140‑1 (MQ=255)                                                                                                          gagaTCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCa                           <  1:562875/140‑1 (MQ=255)                                                                                                          gagaTCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCa                           <  2:31674/140‑1 (MQ=255)                                                                                                           agaTCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCAc                          >  2:978619/1‑140 (MQ=255)                                                                                                           agaTCTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCAc                          >  1:178598/1‑140 (MQ=255)                                                                                                               cTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACtgtg                      <  1:504719/140‑1 (MQ=255)                                                                                                               cTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACtgtg                      <  2:384909/140‑1 (MQ=255)                                                                                                               cTTCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACtgtg                      <  2:121912/140‑1 (MQ=255)                                                                                                                 tCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCa                           <  1:1055592/133‑1 (MQ=255)                                                                                                                 tCCGTCAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCa                           >  2:1055592/1‑133 (MQ=255)                                                                                                                      cAGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACTGTGTACCCCt               >  2:365165/1‑140 (MQ=255)                                                                                                                       aGCTTTGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACTGTGTACCCCTg              >  1:882745/1‑140 (MQ=255)                                                                                                                          tttGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACTGTGTACCCCTGAAt           >  1:13987/1‑140 (MQ=255)                                                                                                                            tGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACTGTGTACCCCTGAATCt         >  2:836737/1‑140 (MQ=255)                                                                                                                            tGGCATCTCCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCA‑CGTGCTGGCCTCAACCACTGTGTACCCCTGAATCTg        >  1:324102/1‑140 (MQ=255)                                                                                                                                   tcCGGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACTGTGTACCCCTGAATCTGCGCCTg  <  1:979488/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CGCCAGCGTTTCGAGAATGGTGCGCATATCGCGAATCGGTACTTTTTCATCGAGGAGATTTTGCAGCACTTTATGCAGTGTGGTGAGCGTGACGACGCCAGGAACGAGATCTTCCGTCAGCTTTGGCATCTCCTGGGCGACGCGATCCAACAGCTGTTGCGCCTCCTGACGACCAAACAGCTCTGCGGCATGCTGGCTAATGAGGTGGTTAAGATGCGTTGCTACCACCGTGCTGGCCTCAACCACTGTGTACCCCTGAATCTGCGCCTG  >  NC_000913/1963365‑1963634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑