Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	134,761	C→T	intergenic (+11/+27)	yacL → / ← speD	UPF0231 family protein YacL/S‑adenosylmethionine decarboxylase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	134,761	0	C	T	100.0%	216.8 / NA	61	intergenic (+11/+27)	yacL/speD	UPF0231 family protein YacL/S‑adenosylmethionine decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/31);  total (30/31)

GGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGT  >  NC_000913/134624‑134894                                                                                                                                          |                                                                                                                                      ggAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCtggtgg                                                                                                                                     <  2:902012/140‑1 (MQ=255)   aaTTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAg                                                                                                                                   <  2:583857/140‑1 (MQ=255)       cGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTg                                                                                                                               <  2:446232/140‑1 (MQ=255)          tGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGa                                                                                                                            >  2:620059/1‑140 (MQ=255)          tGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGAAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGa                                                                                                                            >  1:667274/1‑140 (MQ=255)            gCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGc                                                                                                                          <  1:350083/140‑1 (MQ=255)            gCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCAGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGc                                                                                                                          <  1:491864/140‑1 (MQ=255)                 gAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGc                                                                                                                     <  1:1125666/140‑1 (MQ=255)                    aTGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCag                                                                                                                  <  2:260724/140‑1 (MQ=255)                       gAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGc                                                                                                               <  1:963863/140‑1 (MQ=255)                             gggATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGc                                                                                                                          >  2:506848/1‑123 (MQ=255)                             gggATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGc                                                                                                                          <  1:506848/123‑1 (MQ=255)                                 tGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAg                                                                                                     >  2:920326/1‑140 (MQ=255)                                       actacGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCa                                                                                               >  1:702534/1‑140 (MQ=255)                                        ctacGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAg                                                                                                     >  2:867563/1‑133 (MQ=255)                                        ctacGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAg                                                                                                     <  1:867563/133‑1 (MQ=255)                                           cgacgaAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAAc                                                                                                       <  2:86641/128‑1 (MQ=255)                                           cgacgaAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAAc                                                                                                       >  1:86641/1‑128 (MQ=255)                                             acgaAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTgc                                                                                         >  2:228698/1‑140 (MQ=255)                                             acgaAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTgc                                                                                         >  1:311018/1‑140 (MQ=255)                                             acgaAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTAGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTgc                                                                                         >  2:1070656/1‑140 (MQ=255)                                              cgaAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTaaa                                                                                                        <  1:156443/124‑1 (MQ=255)                                              cgaAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTaaa                                                                                                        >  2:156443/1‑124 (MQ=255)                                               gaagaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTgcgc                                                                                       <  1:920326/140‑1 (MQ=255)                                               gaagaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTgcgc                                                                                       <  2:702534/140‑1 (MQ=255)                                               gaagaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTgcgc                                                                                       <  1:406549/140‑1 (MQ=255)                                               gaagaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTACAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTgcgc                                                                                       <  1:822832/140‑1 (MQ=255)                                                aagaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGcc                                                                                      >  1:594066/1‑140 (MQ=255)                                                aagaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGcc                                                                                      <  1:340054/140‑1 (MQ=255)                                                aagaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGcc                                                                                      >  1:398459/1‑140 (MQ=255)                                                 agaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGccc                                                                                     <  2:1166565/140‑1 (MQ=255)                                                  gaaAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCg                                                                                    >  1:514545/1‑140 (MQ=255)                                                           tCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGAAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAAtc                                                                           <  2:667274/140‑1 (MQ=255)                                                              cTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTcg                                                                        <  1:470475/140‑1 (MQ=255)                                                                aTGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTcgcg                                                                      <  2:398459/140‑1 (MQ=255)                                                                  gcggcgTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCa                                                                    <  1:228698/140‑1 (MQ=255)                                                                   cggcgTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCAt                                                                   >  2:1128059/1‑140 (MQ=255)                                                                      cgTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCAtttc                                                                >  2:274906/1‑140 (MQ=255)                                                                      cgTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCAtttc                                                                >  1:564652/1‑140 (MQ=255)                                                                        ttGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCAtttctt                                                              >  1:63368/1‑140 (MQ=255)                                                                         tGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCAtttcttt                                                             <  2:311018/140‑1 (MQ=255)                                                                          gAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCAtttctttc                                                            <  1:274906/140‑1 (MQ=255)                                                                                  tCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCAcagcgc                                                    >  2:762952/1‑140 (MQ=255)                                                                                       aGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCgg                                               >  2:1020662/1‑140 (MQ=255)                                                                                         gTCGTGGCGGCTTACCGCAATTTCGT‑CAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTaa                                            >  2:1031974/1‑140 (MQ=255)                                                                                                           aaTTTCGTGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTg                           <  1:762952/140‑1 (MQ=255)                                                                                                                  tGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTa                    >  2:554229/1‑140 (MQ=255)                                                                                                                  tGCAGCAGAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTa                    >  1:1011890/1‑140 (MQ=255)                                                                                                                      gcagAAGTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTc                <  2:63368/140‑1 (MQ=255)                                                                                                                            gTAAATAAATCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCgg          >  2:929881/1‑140 (MQ=255)                                                                                                                             taaataaaTCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGt         <  2:1011890/140‑1 (MQ=255)                                                                                                                              aaataaatCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGtt        <  2:564652/140‑1 (MQ=255)                                                                                                                              aaataaatCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGtt        <  2:514545/140‑1 (MQ=255)                                                                                                                              aaataaatCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGtt        <  1:554229/140‑1 (MQ=255)                                                                                                                               aataaatCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGttt       >  2:1094713/1‑140 (MQ=255)                                                                                                                                ataaatCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTg      >  2:545024/1‑140 (MQ=255)                                                                                                                                 taaatCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTgg     >  1:955002/1‑140 (MQ=255)                                                                                                                                    atCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGtt        >  2:1040525/1‑134 (MQ=255)                                                                                                                                    atCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGtt        <  1:1040525/134‑1 (MQ=255)                                                                                                                                    atCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt  >  2:166770/1‑140 (MQ=255)                                                                                                                                    atCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt  >  1:1151263/1‑140 (MQ=255)                                                                                                                                    atCTGGTGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGgtgt  <  1:1094713/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GGAATTCGCTGGCGATGAAATGGAAGAGGGGATGAACTACTACGACGAAGAAAGCCTGTCGCTATGCGGCGTTGAGGATTTTCTGCAGGTCGTGGCGGCTTACCGCAATTTCGTGCAGCAGAAGTAAATAAATCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGT  >  NC_000913/134624‑134894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑