Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,161	T→C	D87G (GAC→GGC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,161	0	T	C	100.0%	194.2 / NA	54	D87G (GAC→GGC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/29);  total (25/29)

ATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCG  >  NC_000913/2339025‑2339293                                                                                                                                         |                                                                                                                                     aTGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCAt                                                                                                                                   >  1:952208/1‑140 (MQ=255) aTGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCAt                                                                                                                                   >  1:728881/1‑140 (MQ=255) aTGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCAt                                                                                                                                   >  1:480759/1‑140 (MQ=255)        aTTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGAccgc                                                                                                                            >  1:1185273/1‑140 (MQ=255)        aTTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGAccgc                                                                                                                            >  1:164319/1‑140 (MQ=255)        aTTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGAccgc                                                                                                                            >  1:549857/1‑140 (MQ=255)         ttttCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGAccgcc                                                                                                                           >  2:599753/1‑140 (MQ=255)            tCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAg                                                                                                                        <  2:377559/140‑1 (MQ=255)             cGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTCCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGt                                                                                                                       <  2:1184313/140‑1 (MQ=255)             cGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGt                                                                                                                       <  2:54269/140‑1 (MQ=255)              gCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTc                                                                                                                      <  1:658776/140‑1 (MQ=255)                   aCGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGAccgccg                                                                                                                          <  1:529341/131‑1 (MQ=255)                   aCGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGAccgccg                                                                                                                          >  2:529341/1‑131 (MQ=255)                       aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAAGCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATgggg                                                                                                             <  2:1154458/140‑1 (MQ=255)                       aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATgggg                                                                                                             <  1:919588/140‑1 (MQ=255)                       aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATgggg                                                                                                             <  2:549857/140‑1 (MQ=255)                       aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATgggg                                                                                                             <  2:164319/140‑1 (MQ=255)                       aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATgggg                                                                                                             <  2:728881/140‑1 (MQ=255)                         ttCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGAt                                                                                                           <  1:349245/140‑1 (MQ=255)                          tCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATg                                                                                                          <  2:692455/140‑1 (MQ=255)                                   cGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATgggg                                                                                                             >  1:339161/1‑128 (MQ=255)                                   cGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATgggg                                                                                                             <  2:339161/128‑1 (MQ=255)                                      aTTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGAt                                                                                              <  2:1171206/140‑1 (MQ=255)                                        tGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCg                                                                                                >  1:202571/1‑136 (MQ=255)                                        tGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCg                                                                                                <  2:202571/136‑1 (MQ=255)                                                        ccGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACg                                                                            >  2:1226567/1‑140 (MQ=255)                                                        ccGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACg                                                                            >  2:974017/1‑140 (MQ=255)                                                           tCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                         <  1:599753/140‑1 (MQ=255)                                                               tAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCGGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGCCACGGGCAGAt                                                                     >  1:295979/1‑140 (MQ=255)                                                                      gAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                              <  1:545883/140‑1 (MQ=255)                                                                      gAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                              <  1:1232949/140‑1 (MQ=255)                                                                      gAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                              <  2:1185273/140‑1 (MQ=255)                                                                            aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCGTGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCtt                                                        >  2:1080869/1‑140 (MQ=255)                                                                            aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                              <  1:763795/134‑1 (MQ=255)                                                                            aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                              >  2:763795/1‑134 (MQ=255)                                                                            aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCtt                                                        <  1:974017/140‑1 (MQ=255)                                                                                      tCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTc                                              >  2:874966/1‑140 (MQ=255)                                                                                           cAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGc                                         <  1:740434/140‑1 (MQ=255)                                                                                            aGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGcc                                        <  1:1226567/140‑1 (MQ=255)                                                                                               atatAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAg                                     >  2:180958/1‑140 (MQ=255)                                                                                                tataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGt                                    >  2:118670/1‑140 (MQ=255)                                                                                                tataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGt                                    >  1:1018054/1‑140 (MQ=255)                                                                                                   aaCGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAg                                     >  1:845273/1‑136 (MQ=255)                                                                                                   aaCGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAg                                     <  2:845273/136‑1 (MQ=255)                                                                                                     cGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGtt                               >  2:79953/1‑140 (MQ=255)                                                                                                      gCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTc                              <  2:1018054/140‑1 (MQ=255)                                                                                                         gCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATg                           <  1:874966/140‑1 (MQ=255)                                                                                                          cGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATgg                          >  2:1208701/1‑140 (MQ=255)                                                                                                            agaATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCg                        <  1:947704/140‑1 (MQ=255)                                                                                                            agaATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCg                        <  1:1218366/140‑1 (MQ=255)                                                                                                                           tGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTTAAGTACGCGACgg         >  1:712870/1‑140 (MQ=255)                                                                                                                           tGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcgcg             >  2:1159354/1‑136 (MQ=255)                                                                                                                           tGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcgcg             <  1:1159354/136‑1 (MQ=255)                                                                                                                                aagATCGGGCCAGAGACCGTCGAGTCACCATGGGGATGGTATTTACCGATTACGTCCCCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTAc    <  2:295979/138‑1 (MQ=255)                                                                                                                                  gATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCg  >  1:717320/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     ATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCG  >  NC_000913/2339025‑2339293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑