Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,340,476	T→C	K1232E (AAA→GAA)	yfaL ←	putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,340,476	0	T	C	100.0%	247.6 / NA	68	K1232E (AAA→GAA)	yfaL	putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/32);  total (36/32)

TCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGG  >  NC_000913/2340340‑2340611                                                                                                                                         |                                                                                                                                        tCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTg                                                                                                                                      >  2:19083/1‑140 (MQ=255)        tACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTgccacgca                                                                                                                               >  1:230431/1‑139 (MQ=255)         aCAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTgccacgcca                                                                                                                              >  1:725833/1‑140 (MQ=255)                 tCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCcg                                                                                                                      <  2:265554/140‑1 (MQ=255)                  ccccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACACTGCcgc                                                                                                                     >  2:439019/1‑140 (MQ=255)                   cccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCcgcg                                                                                                                    >  2:912732/1‑140 (MQ=255)                    ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCgcgc                                                                                                                   >  1:509807/1‑140 (MQ=255)                     cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACACTGCCGCGCa                                                                                                                  <  1:439019/140‑1 (MQ=255)                         ggCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGa                                                                                                              <  1:35855/140‑1 (MQ=255)                               aTAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCg                                                                                                        >  1:421352/1‑140 (MQ=255)                                 aaGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGct                                                                                                      <  2:348526/140‑1 (MQ=255)                                   ggCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctga                                                                                                    <  2:421352/140‑1 (MQ=255)                                    gCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgac                                                                                                   <  1:912732/140‑1 (MQ=255)                                        ttACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACAGTCGCTGACTGat                                                                                               >  1:465709/1‑140 (MQ=255)                                        ttACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGat                                                                                               >  2:473013/1‑140 (MQ=255)                                         tACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctga                                                                                                    <  2:820105/134‑1 (MQ=255)                                         tACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctga                                                                                                    >  1:820105/1‑134 (MQ=255)                                         tACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGata                                                                                              >  2:1032542/1‑140 (MQ=255)                                          aCGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGatat                                                                                             <  1:19083/140‑1 (MQ=255)                                           cgccgcATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATAtt                                                                                            <  1:1106534/140‑1 (MQ=255)                                              cgcATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGcc                                                                                         >  1:1030156/1‑140 (MQ=255)                                               gcATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGccc                                                                                        >  1:300996/1‑140 (MQ=255)                                               gcATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGccc                                                                                        >  1:89908/1‑140 (MQ=255)                                               gcATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGccc                                                                                        >  1:628000/1‑140 (MQ=255)                                                  tCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTg                                                                                     >  2:1206236/1‑140 (MQ=255)                                                   ccGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGa                                                                                    <  1:1206236/140‑1 (MQ=255)                                                       cAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACAGTCGCTGACTGATATTGCCCGTGACTcc                                                                                <  2:465709/140‑1 (MQ=255)                                                       cAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTcc                                                                                <  2:725833/140‑1 (MQ=255)                                                       cAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTcc                                                                                <  1:1157447/140‑1 (MQ=255)                                                       cAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTcc                                                                                <  2:628000/140‑1 (MQ=255)                                                          ccGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCAc                                                                             >  2:130597/1‑140 (MQ=255)                                                            gTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACtt                                                                           <  1:1032542/140‑1 (MQ=255)                                                             tGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACttt                                                                          >  2:545461/1‑140 (MQ=255)                                                             tGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACttt                                                                          >  2:229321/1‑140 (MQ=255)                                                             tGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCAACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACttt                                                                          <  2:230431/140‑1 (MQ=255)                                                                           tGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGtt                                                            >  2:731316/1‑140 (MQ=255)                                                                             aTTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGc                                                          >  1:366232/1‑140 (MQ=255)                                                                             aTTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGc                                                          >  2:1233257/1‑140 (MQ=255)                                                                              ttACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCt                                                         >  2:809248/1‑140 (MQ=255)                                                                                aCCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTc                                                       >  2:231597/1‑140 (MQ=255)                                                                                     ttCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGc                                                  >  1:438907/1‑140 (MQ=255)                                                                                     ttCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGc                                                  >  1:605595/1‑140 (MQ=255)                                                                                      tCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCa                                                 <  1:130597/140‑1 (MQ=255)                                                                                      tCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCa                                                 <  1:1233257/140‑1 (MQ=255)                                                                                      tCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCa                                                 <  2:605595/140‑1 (MQ=255)                                                                                       cACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCAt                                                >  1:491634/1‑140 (MQ=255)                                                                                       cACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCAt                                                >  2:863993/1‑140 (MQ=255)                                                                                        aCCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATc                                               <  2:509807/140‑1 (MQ=255)                                                                                          cGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGt                                             <  2:438907/140‑1 (MQ=255)                                                                                          cGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGt                                             <  1:731316/140‑1 (MQ=255)                                                                                            tCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCa                                           >  2:421760/1‑140 (MQ=255)                                                                                              aTCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACt                                         >  2:56187/1‑140 (MQ=255)                                                                                                cGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGa                                       <  2:971720/140‑1 (MQ=255)                                                                                                cGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGa                                       <  1:863993/140‑1 (MQ=255)                                                                                                        aCCCTGCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGtgctg                               >  2:434931/1‑140 (MQ=255)                                                                                                             gCCGTCTGGGCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATc                          >  2:943511/1‑140 (MQ=255)                                                                                                              ccGTCTGGGCAAAATCATCAATCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATcc                         >  2:1037041/1‑140 (MQ=255)                                                                                                                     ggCAAAATCATCACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCaa                  <  1:520018/140‑1 (MQ=255)                                                                                                                           atcatcACTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCg            <  2:300996/140‑1 (MQ=255)                                                                                                                            tcatcaCTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGt           <  2:366232/140‑1 (MQ=255)                                                                                                                            tcatcaCTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGt           <  2:1128896/140‑1 (MQ=255)                                                                                                                               tcaCTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGa        >  2:70203/1‑140 (MQ=255)                                                                                                                                caCTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGaa       <  1:70203/140‑1 (MQ=255)                                                                                                                                caCTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGaa       <  1:56187/140‑1 (MQ=255)                                                                                                                                caCTCCCTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGaa       <  1:1069454/140‑1 (MQ=255)                                                                                                                                     ccTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGaa       <  2:438288/135‑1 (MQ=255)                                                                                                                                     ccTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGaa       >  1:438288/1‑135 (MQ=255)                                                                                                                                     ccTTCCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATgggg  >  1:433135/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGG  >  NC_000913/2340340‑2340611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑