Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,355,067	G→A	R268H (CGC→CAC)	glpB →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,355,067	0	G	A	100.0%	126.8 / NA	37	R268H (CGC→CAC)	glpB	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/16);  total (21/16)

GCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCA  >  NC_000913/2354928‑2355201                                                                                                                                            |                                                                                                                                       gCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTgcg                                                                                                                                        <  2:47649/140‑1 (MQ=255)              tGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCgtg                                                                                                                           <  2:23467/140‑1 (MQ=255)               ggCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCgtgt                                                                                                                          >  2:79807/1‑140 (MQ=255)                gCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTgg                                                                                                                               <  1:123315/134‑1 (MQ=255)                gCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTgg                                                                                                                               >  2:123315/1‑134 (MQ=255)                gCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGtgtg                                                                                                                         >  1:1011224/1‑140 (MQ=255)                  cGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGa                                                                                                                       <  2:738439/140‑1 (MQ=255)                    aTGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATg                                                                                                                     <  2:399537/140‑1 (MQ=255)                          aaCTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGc                                                                                                               >  2:127887/1‑140 (MQ=255)                           aCTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCg                                                                                                              <  1:1141806/140‑1 (MQ=255)                               tgGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGa                                                                                                          >  2:1165418/1‑140 (MQ=255)                               tgGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGa                                                                                                          <  1:420544/140‑1 (MQ=255)                                 gCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAg                                                                                                        >  2:809067/1‑140 (MQ=255)                                       gttgAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGaaaa                                                                                                  >  2:274927/1‑140 (MQ=255)                                             tgaaAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGa                                                                                            <  1:809067/140‑1 (MQ=255)                                               aaaaaCTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGAcc                                                                                          <  1:70181/140‑1 (MQ=255)                                                 aaaCTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTg                                                                                        >  2:1115590/1‑140 (MQ=255)                                                  aaCTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGt                                                                                       >  1:1102410/1‑140 (MQ=255)                                                  aaCTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGt                                                                                       <  1:1165418/140‑1 (MQ=255)                                                    cTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTaa                                                                                     <  1:127887/140‑1 (MQ=255)                                                         ttGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATg                                                                                >  2:799138/1‑140 (MQ=255)                                                                  gATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGa                                                                       >  2:327342/1‑140 (MQ=255)                                                                      cTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGt                                                                         <  1:474724/134‑1 (MQ=255)                                                                      cTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGt                                                                         >  2:474724/1‑134 (MQ=255)                                                                      cTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGa                                                                   >  2:347955/1‑140 (MQ=255)                                                                                        ccTTCCGTGCTGGGCATGCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCCGGCTGGCGTGTGGATGCGGGGCGATTAAGTGAAAAAAGTGGCGTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCATTCa                                                 <  2:673669/140‑1 (MQ=255)                                                                                            ccGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGcc                                             <  1:274927/140‑1 (MQ=255)                                                                                                                cAAAACCAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACg                         >  1:712635/1‑140 (MQ=255)                                                                                                                     ccAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTcg                    <  2:1011224/140‑1 (MQ=255)                                                                                                                     ccAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTcg                    <  2:541852/140‑1 (MQ=255)                                                                                                                     ccAGTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAAAGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTcg                    <  2:1102410/140‑1 (MQ=255)                                                                                                                        gTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCgg                 >  1:687020/1‑140 (MQ=255)                                                                                                                        gTTACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCgg                 >  1:24704/1‑140 (MQ=255)                                                                                                                          tACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGtt               >  1:1139459/1‑140 (MQ=255)                                                                                                                          tACAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGtt               >  2:1037514/1‑140 (MQ=255)                                                                                                                           aCAGCGCCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTtc              >  1:152106/1‑140 (MQ=255)                                                                                                                               cgcCAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGc          >  2:805996/1‑140 (MQ=255)                                                                                                                                 ccAGTTTGTGCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCa        >  1:290435/1‑140 (MQ=255)                                                                                                                                       tgtgCACCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCa  <  1:79807/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                       GCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCA  >  NC_000913/2354928‑2355201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑