Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,376,497	A→G	V108A (GTG→GCG)	menB ←	1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,376,497	0	A	G	100.0%	112.6 / NA	33	V108A (GTG→GCG)	menB	1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

CCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCACATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCACCAGTCAGAATGATCACGCCGATGTTGT  >  NC_000913/2376362‑2376620                                                                                                                                        |                                                                                                                            ccGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCAtt                                                                                                                         >  1:785798/1‑140 (MQ=255) ccGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCAtt                                                                                                                         <  1:870310/140‑1 (MQ=255)    aaGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAg                                                                                                                      >  2:951535/1‑140 (MQ=255)          gCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATg                                                                                                                >  1:899991/1‑140 (MQ=255)                   gCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGa                                                                                                       <  2:688904/140‑1 (MQ=255)                     cgcgATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAat                                                                                                     <  1:878363/140‑1 (MQ=255)                      gcgATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAatc                                                                                                    <  1:678544/140‑1 (MQ=255)                         aTAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAatcatc                                                                                                 >  1:1075625/1‑140 (MQ=255)                                ggccGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGATACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTCCGCCGGAATCATCTTTATAg                                                                                          <  2:1100025/137‑1 (MQ=255)                                   cGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAgccg                                                                                       <  2:961470/140‑1 (MQ=255)                                       caacatGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGt                                                                                   >  2:494201/4‑140 (MQ=255)                                         tcatGTGCAGACGGTGCCCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTaa                                                                                 <  2:899991/140‑1 (MQ=255)                                            tgtgCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATca                                                                              >  1:777123/1‑140 (MQ=255)                                                 aGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACg                                                                         <  2:1075625/140‑1 (MQ=255)                                                  gAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGc                                                                        >  2:88909/1‑140 (MQ=255)                                                   aaCGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCa                                                                       >  1:149038/1‑140 (MQ=255)                                                   aaCGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCa                                                                       >  1:277829/1‑140 (MQ=255)                                                    aCGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGGATCATCTTTATAGCCGCCGTAATCACCACGCAc                                                                      >  2:384760/1‑140 (MQ=255)                                                            gccgccgATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGt                                                              <  1:494201/140‑1 (MQ=255)                                                             ccgccgATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTc                                                             >  2:590933/1‑140 (MQ=255)                                                                           gccagccaCCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGGATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGa                                               <  1:384760/140‑1 (MQ=255)                                                                           gccagccaCCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGa                                               <  1:951535/140‑1 (MQ=255)                                                                            ccagccaCCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGaa                                              >  1:611747/1‑140 (MQ=255)                                                                             cagccaCCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGAAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAAc                                             <  1:923451/140‑1 (MQ=255)                                                                                ccaccaTCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCt                                          >  2:851195/1‑140 (MQ=255)                                                                                            cAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCa                              <  2:402335/140‑1 (MQ=255)                                                                                            cAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCa                              <  2:785798/140‑1 (MQ=255)                                                                                            cAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCa                              <  2:830893/140‑1 (MQ=255)                                                                                            cAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCa                              <  1:88909/140‑1 (MQ=255)                                                                                                     tCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCACCAGTCAGa                     <  2:149038/140‑1 (MQ=255)                                                                                                                    tCTGACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCACCAGTCAGAATGATCACGCCGAtg      <  2:277829/140‑1 (MQ=255)                                                                                                                       gACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCACCAGTCAGAATGATCACGCCGAtgttg   >  2:20583/1‑140 (MQ=255)                                                                                                                       gACGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCACCAGTCAGAATGATCACGCCGAtgttg   >  1:151156/1‑140 (MQ=255)                                                                                                                        aCGCTGGAAGTCCAGCGCATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCACCAGTCAGAATGATCACGCCGAtgttgt  >  1:1128629/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                            CCGAAGATGGCATTATCTGCCGCGATAGTCAGGTCGCACATCATGTGCAGAACGTGACCGCCGCCGATGGAGTAGCCAGCCACCATCGCGACAACCGGTTTCGGACAGGTACGGATCTGACGCTGGAAGTCCAGCACATTCAGGTGATGTACGCCGGAATCATCTTTATAGCCGCCGTAATCACCACGCACTTTCTGGTCACCACCGGAGCAGAACGCTTTATCGCCTGCACCAGTCAGAATGATCACGCCGATGTTGT  >  NC_000913/2376362‑2376620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑