Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,445,843	G→A	A181V (GCG→GTG)	mepA ←	peptidoglycan DD‑endopeptidase/peptidoglycan LD‑endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,445,843	0	G	A	100.0%	243.1 / NA	71	A181V (GCG→GTG)	mepA	peptidoglycan DD‑endopeptidase/peptidoglycan LD‑endopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/36);  total (35/36)

CACACTCCAGACTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAA  >  NC_000913/2445715‑2445979                                                                                                                                 |                                                                                                                                         cacaCTCCAGACTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCCACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACa                                                                                                                               >  2:738704/1‑140 (MQ=255)  acacTCCAGACTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGAt                                                                                                                                    <  2:319749/134‑1 (MQ=255)  acacTCCAGACTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGAt                                                                                                                                    >  1:319749/1‑134 (MQ=255)     cTCCAGACTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACaaaaa                                                                                                                           <  2:556953/140‑1 (MQ=255)      tCCAGACTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAAt                                                                                                                          >  1:219363/1‑140 (MQ=255)        cAGACTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATgc                                                                                                                        >  2:790247/1‑140 (MQ=255)        cAGACTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATgc                                                                                                                        >  1:889917/1‑140 (MQ=255)           aCTATCGGCAGGGCAACGTAATCGTACATGTATATGCGCGCGATGCTGGAACCAGGGCCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATTACCGGATTAACAAAAATGcgcg                                                                                                                     >  2:599622/1‑140 (MQ=255)           aCTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGcgcg                                                                                                                     >  1:1131362/1‑140 (MQ=255)           aCTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGcgcg                                                                                                                     >  2:778856/1‑140 (MQ=255)           aCTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGcgcg                                                                                                                     >  1:86800/1‑140 (MQ=255)              aTCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGa                                                                                                                  <  1:738704/140‑1 (MQ=255)              aTCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGa                                                                                                                  <  2:662629/140‑1 (MQ=255)                cGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACg                                                                                                                >  1:736385/1‑140 (MQ=255)                   cAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCt                                                                                                             <  1:683797/140‑1 (MQ=255)                     gggCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCttt                                                                                                           <  1:1202679/140‑1 (MQ=255)                     gggCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCttt                                                                                                           >  2:576334/1‑140 (MQ=255)                     gggCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCttt                                                                                                           >  2:139238/1‑140 (MQ=255)                      ggCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTg                                                                                                          >  1:670863/1‑140 (MQ=255)                           cGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTg                                                                                                     >  1:711994/1‑140 (MQ=255)                           cGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTg                                                                                                     >  1:645860/1‑140 (MQ=255)                             tAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTggg                                                                                                   >  2:958784/1‑140 (MQ=255)                               aTCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGggcg                                                                                                 <  2:736385/140‑1 (MQ=255)                               aTCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGggcg                                                                                                 <  2:889917/140‑1 (MQ=255)                                tCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGggcgg                                                                                                <  1:829543/140‑1 (MQ=255)                                tCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGggcgg                                                                                                <  2:476911/140‑1 (MQ=255)                                tCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGggcgg                                                                                                >  2:783766/1‑140 (MQ=255)                                tCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGggcgg                                                                                                <  2:964198/140‑1 (MQ=255)                                tCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGggcgg                                                                                                <  1:469464/140‑1 (MQ=255)                                             tGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCaa                                                                                   >  2:456470/1‑140 (MQ=255)                                             tGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCaa                                                                                   >  2:836548/1‑140 (MQ=255)                                             tGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCaa                                                                                   >  1:795093/1‑140 (MQ=255)                                             tGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCaa                                                                                   >  1:616685/1‑140 (MQ=255)                                                 cgcgATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTg                                                                               >  1:1195433/1‑140 (MQ=255)                                                 cgcgATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTg                                                                               >  1:26104/1‑140 (MQ=255)                                                  gcgATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGa                                                                              >  2:796786/1‑140 (MQ=255)                                                  gcgATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGa                                                                              >  1:858068/1‑140 (MQ=255)                                                  gcgATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGa                                                                              >  1:428382/1‑140 (MQ=255)                                                  gcgATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGa                                                                              >  2:550024/1‑140 (MQ=255)                                                        cTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAAttt                                                                        <  2:670863/140‑1 (MQ=255)                                                        cTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAAttt                                                                        <  2:54508/140‑1 (MQ=255)                                                        cTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGCGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAAttt                                                                        <  2:219363/140‑1 (MQ=255)                                                             aCCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGc                                                                   <  1:139238/140‑1 (MQ=255)                                                                aGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAAttt                                                                        <  1:524046/132‑1 (MQ=255)                                                                aGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAAttt                                                                        >  2:524046/1‑132 (MQ=255)                                                                aGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTc                                                                >  1:116231/1‑140 (MQ=255)                                                                 gggTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTcc                                                               >  1:592926/1‑140 (MQ=255)                                                                   gTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCcac                                                             <  1:760030/140‑1 (MQ=255)                                                                   gTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCcac                                                             <  1:696023/140‑1 (MQ=255)                                                                   gTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCcac                                                             <  2:645860/140‑1 (MQ=255)                                                                       cACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCg                                                         <  1:783766/140‑1 (MQ=255)                                                                         cTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTg                                                       <  1:958784/140‑1 (MQ=255)                                                                         cTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTg                                                       <  1:790247/140‑1 (MQ=255)                                                                            tGCGCAACCAGTCGCGATCGGTGCACGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGgag                                                    <  1:599622/140‑1 (MQ=255)                                                                               gcAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACa                                                 <  2:116231/140‑1 (MQ=255)                                                                               gcAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACa                                                 <  1:456470/140‑1 (MQ=255)                                                                                      gTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCAAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGgag                                                    <  2:224928/130‑1 (MQ=255)                                                                                      gTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCAAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGgag                                                    >  1:224928/1‑130 (MQ=255)                                                                                             tCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTc                                   <  1:576334/140‑1 (MQ=255)                                                                                                gTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTcgcg                                <  1:550024/140‑1 (MQ=255)                                                                                                gTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTcgcg                                <  2:86800/140‑1 (MQ=255)                                                                                                       cATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACt                         >  1:162275/1‑140 (MQ=255)                                                                                                       cATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACt                         <  1:836548/140‑1 (MQ=255)                                                                                                       cATCAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACt                         <  2:616685/140‑1 (MQ=255)                                                                                                          cAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAg                      >  1:910578/1‑140 (MQ=255)                                                                                                          cAAGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAg                      >  2:234756/1‑140 (MQ=255)                                                                                                            aGGCAAAGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAggc                    <  1:234756/140‑3 (MQ=255)                                                                                                                  aGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGc              >  1:111412/1‑140 (MQ=255)                                                                                                                  aGTTGTTGTTTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGATTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGc              >  2:1099912/1‑140 (MQ=255)                                                                                                                    ttgttgttTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCtt            <  2:26104/140‑1 (MQ=255)                                                                                                                       ttgttTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTg           >  2:260863/1‑138 (MQ=255)                                                                                                                       ttgttTAATCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTg           <  1:260863/138‑1 (MQ=255)                                                                                                                              aTCACCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCaa  <  2:910578/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         CACACTCCAGACTATCGGCAGGGCAACGTAATCGTACATGCATATGCGCGCGATGCTGGAACCAGGGTCGCACTTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAA  >  NC_000913/2445715‑2445979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑