Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,584,434	G→A	G364G (GGC→GGT)	aegA ←	putative oxidoreductase Fe‑S subunit AegA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,584,434	0	G	A	100.0%	198.7 / NA	58	G364G (GGC→GGT)	aegA	putative oxidoreductase Fe‑S subunit AegA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/29);  total (29/29)

AAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCT  >  NC_000913/2584298‑2584565                                                                                                                                         |                                                                                                                                    aaaGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCa                                                                                                                                  <  2:586966/140‑1 (MQ=255)   aGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAaccacc                                                                                                                                <  1:323335/140‑1 (MQ=255)      gAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGAt                                                                                                                             >  1:845520/1‑140 (MQ=255)      gAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGAt                                                                                                                             >  2:954442/1‑140 (MQ=255)       aaTCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGAtt                                                                                                                            >  1:1205014/1‑140 (MQ=255)                agaCATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATgg                                                                                                                   <  1:733951/140‑1 (MQ=255)                agaCATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATgg                                                                                                                   <  2:948410/140‑1 (MQ=255)                agaCATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATgg                                                                                                                   <  1:954442/140‑1 (MQ=255)                agaCATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATgg                                                                                                                   <  1:108969/140‑1 (MQ=255)                agaCATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATgg                                                                                                                   <  2:48401/140‑1 (MQ=255)                  aCATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATgg                                                                                                                   <  1:1118360/138‑1 (MQ=255)                  aCATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATgg                                                                                                                   >  2:1118360/1‑138 (MQ=255)                  aCATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCg                                                                                                                 >  2:1096057/1‑140 (MQ=255)                        ttACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTa                                                                                                           <  2:845520/140‑1 (MQ=255)                            ccACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACAcc                                                                                                       >  1:446732/1‑140 (MQ=255)                              aCTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGt                                                                                                     >  1:1184417/1‑140 (MQ=255)                               cTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGAt                                                                                                                     <  2:705283/123‑1 (MQ=255)                               cTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGAt                                                                                                                     >  1:705283/1‑123 (MQ=255)                                ttCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTa                                                                                                           >  1:576392/1‑132 (MQ=255)                                ttCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTa                                                                                                           <  2:908328/132‑1 (MQ=255)                                ttCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTa                                                                                                           >  1:908328/1‑132 (MQ=255)                                ttCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTa                                                                                                           <  2:576392/132‑1 (MQ=255)                                ttCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCa                                                                                                   >  2:1095250/1‑140 (MQ=255)                                ttCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCa                                                                                                   >  2:592576/1‑140 (MQ=255)                                ttCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCa                                                                                                   >  1:614149/1‑140 (MQ=255)                                       ttGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCAc                                                                                            <  2:1192585/140‑1 (MQ=255)                                            ttCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCAt                                                                                       >  2:987030/1‑140 (MQ=255)                                            ttCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCAt                                                                                       >  1:577444/1‑140 (MQ=255)                                            ttCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCAt                                                                                       >  2:116868/1‑140 (MQ=255)                                            ttCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCAt                                                                                       >  1:129602/1‑140 (MQ=255)                                               gAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTg                                                                                     <  2:611365/139‑1 (MQ=255)                                               gAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTg                                                                                     >  1:611365/1‑139 (MQ=255)                                                   tGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGt                                                                                <  1:637186/140‑1 (MQ=255)                                                     aaTCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCa                                                                              >  1:269210/1‑140 (MQ=255)                                                      aTCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAg                                                                             <  2:129602/140‑1 (MQ=255)                                                           cATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACAt                                                                        <  2:269210/140‑1 (MQ=255)                                                             tCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATcc                                                                      >  1:1035443/1‑140 (MQ=255)                                                                cgcTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGca                                                                   >  2:619704/1‑140 (MQ=255)                                                                cgcTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGca                                                                   <  1:1095250/140‑1 (MQ=255)                                                                cgcTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGca                                                                   <  2:149517/140‑1 (MQ=255)                                                                cgcTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGca                                                                   <  2:614149/140‑1 (MQ=255)                                                                   tGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAg                                                                >  2:1189330/1‑140 (MQ=255)                                                                              cGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGc                                                     <  2:748695/140‑1 (MQ=255)                                                                              cGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGc                                                     <  1:962971/140‑1 (MQ=255)                                                                                 cGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCgg                                                  >  2:621144/1‑140 (MQ=255)                                                                                      tGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTg                                             >  1:1073078/1‑140 (MQ=255)                                                                                                 aTTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATc                                  >  1:923419/1‑140 (MQ=255)                                                                                                  tttATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCACACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCg                                 <  2:69148/140‑1 (MQ=255)                                                                                                          gTTTGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGc                         >  2:820360/1‑140 (MQ=255)                                                                                                           tttGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCa                              <  2:231755/134‑1 (MQ=255)                                                                                                           tttGAAAGAAGGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCa                              >  1:231755/1‑134 (MQ=255)                                                                                                                aagaagGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGc                         >  2:247469/1‑134 (MQ=255)                                                                                                                aagaagGAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGc                         <  1:247469/134‑1 (MQ=255)                                                                                                                     ggAATGCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACttt              >  2:1146397/1‑140 (MQ=255)                                                                                                                          gCCGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGa         <  1:1189330/140‑1 (MQ=255)                                                                                                                           ccGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGAc        <  2:1035443/140‑1 (MQ=255)                                                                                                                            cGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACa       <  1:1096057/140‑1 (MQ=255)                                                                                                                            cGAAAGTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTACCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACa       <  1:987030/140‑1 (MQ=255)                                                                                                                                 gTGAGCAAACCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCt  <  1:820360/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    AAAGCGAATCCAAAGAGACATCTTTACCCACTTCACAATTGAGTTCGAAGTGAATCCCCATCGCGCTGAAGATTTCCCGACGGCGTGCCAGCAGGGATTTATCCAGTTTGAAAGAAGGAATGCCGAAAGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCT  >  NC_000913/2584298‑2584565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑