Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,783,509	T→C	intergenic (‑205/+129)	pinH ← / ← ypjB	putative invertase fragment/DUF5508 domain‑containing protein YpjB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,783,509	0	T	C	100.0%	202.6 / NA	56	intergenic (‑205/+129)	pinH/ypjB	putative invertase fragment/DUF5508 domain‑containing protein YpjB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/33);  total (23/33)

GTAAGATCAAAAAACTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGTAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTACGTTATTATCTCTTTGTAAAAATTGCCATTTATTAATCATTG  >  NC_000913/2783379‑2783643                                                                                                                                   |                                                                                                                                        gTAAGATCAAAAAACTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCttt                                                                                                                                <  2:718153/140‑1 (MQ=255) gTAAGATCAAAAAACTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCttt                                                                                                                                <  1:157719/140‑1 (MQ=255)  tAAGATCAAAAAACTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCt                                                                                                                                  >  2:738560/1‑137 (MQ=255)  tAAGATCAAAAAACTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCt                                                                                                                                  <  1:738560/137‑1 (MQ=255)     gATCAAAAAACTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCt                                                                                                                            <  1:801254/140‑1 (MQ=255)     gATCAAAAAACTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCt                                                                                                                            <  2:1052304/140‑1 (MQ=255)     gATCAAAAAACTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCt                                                                                                                            >  2:378287/1‑140 (MQ=255)          aaaaaCTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCGAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGt                                                                                                                       >  1:494753/1‑140 (MQ=255)          aaaaaCTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGt                                                                                                                       >  1:1039046/1‑140 (MQ=255)          aaaaaCTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGt                                                                                                                       >  2:571102/1‑140 (MQ=255)             aaCTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGt                                                                                                                    <  1:370952/140‑1 (MQ=255)             aaCTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGt                                                                                                                    <  2:1039046/140‑1 (MQ=255)               cTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCGAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGa                                                                                                                  <  2:494753/140‑1 (MQ=255)                  tCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGacac                                                                                                               >  1:1098307/1‑140 (MQ=255)                        aGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAAt                                                                                                         <  2:397629/140‑1 (MQ=255)                            aaGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGAt                                                                                                     <  1:937001/140‑1 (MQ=255)                             aGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATg                                                                                                    >  2:1200766/1‑140 (MQ=255)                             aGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATg                                                                                                    <  2:481885/140‑1 (MQ=255)                             aGGAAAGTTGAACCAGACATTAGATGAAATATTTCAAACAATTACAGCACCAATTCAGTCACTGCCAACCCACCAAATAAATCAAGGGGTTACATCAAAACGCAGCCCCTTTTTCTTTGATAGTGACACTAAAATAGATg                                                                                                    <  2:1161812/140‑1 (MQ=255)                               gAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCTACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTa                                                                                                  <  1:416780/140‑1 (MQ=255)                                 aaGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAgt                                                                                                <  1:571102/140‑1 (MQ=255)                                    ttGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTa                                                                                                  <  2:1230556/135‑1 (MQ=255)                                    ttGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTa                                                                                                  >  1:1230556/1‑135 (MQ=255)                                    ttGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGtgtg                                                                                             <  2:1219087/140‑1 (MQ=255)                                    ttGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGtgtg                                                                                             <  2:426244/140‑1 (MQ=255)                                    ttGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGtgtg                                                                                             <  2:906315/140‑1 (MQ=255)                                    ttGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGtgtg                                                                                             <  1:378287/140‑1 (MQ=255)                                         ccAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTgaagaa                                                                                        >  1:386668/1‑140 (MQ=255)                                           aGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGaata                                                                                      <  1:363643/140‑1 (MQ=255)                                                    aTGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGttt                                                                             <  2:1098307/140‑1 (MQ=255)                                                       aaaTATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACt                                                                          >  1:749910/1‑140 (MQ=255)                                                       aaaTATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACt                                                                          <  2:135484/140‑1 (MQ=255)                                                        aaTATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACt                                                                          <  2:613929/139‑1 (MQ=255)                                                        aaTATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACt                                                                          >  1:613929/1‑139 (MQ=255)                                                           atTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACtcaat                                                                      <  1:748970/140‑1 (MQ=255)                                                           atTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACtcaat                                                                      <  2:288250/140‑1 (MQ=255)                                                             ttCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACtcaatca                                                                    <  1:326097/140‑1 (MQ=255)                                                               cAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTcaatcaat                                                                  <  2:749910/140‑1 (MQ=255)                                                                 aCCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACtcaat                                                                      >  1:612875/1‑134 (MQ=255)                                                                 aCCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACtcaat                                                                      <  2:612875/134‑1 (MQ=255)                                                                            cACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTgat                                                    >  2:1141489/1‑138 (MQ=255)                                                                            cACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGtt                                                    <  2:902812/140‑1 (MQ=255)                                                                                 aTTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAAt                                               >  1:471549/1‑140 (MQ=255)                                                                                 aTTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAAt                                               >  2:1147378/1‑140 (MQ=255)                                                                                 aTTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAAt                                               >  1:375374/1‑140 (MQ=255)                                                                                     aGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTAc                                           <  1:1147378/140‑1 (MQ=255)                                                                                     aGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTAc                                           <  2:386668/140‑1 (MQ=255)                                                                                     aGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTAc                                           <  2:300928/140‑1 (MQ=255)                                                                                      gTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCCTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTACg                                          >  2:626944/1‑140 (MQ=255)                                                                                            gCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTACGttatta                                    >  2:1016749/1‑140 (MQ=255)                                                                                             ccAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAATAAATACATATTGTTTCAATCTACGttatta                                    >  2:818294/1‑140 (MQ=255)                                                                                               aGCCCACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTACGTTATTAtct                                 <  1:1141489/140‑1 (MQ=255)                                                                                                 cccACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTAc                                           >  1:1090144/1‑128 (MQ=255)                                                                                                 cccACCAAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTAc                                           <  2:1090144/128‑1 (MQ=255)                                                                                                     ccaAATAAATCAAGGGGTTACATGAAAACGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTACGTTATTATCTCTTTGt                           >  2:237865/1‑140 (MQ=255)                                                                                                                             aaaaCGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTACGTTATTATCTCTTTGTAAAAATTGCCATTTATTAATCAtt   >  1:1178129/1‑140 (MQ=255)                                                                                                                              aaaCGCAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTACGTTATTATCTCTTTGTAAAAATTGCCATTTATTAATCATTg  >  2:1011363/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        GTAAGATCAAAAAACTTTCAGGCAGCTAAGGAAAGTTGAACCAGACATTAGATGAAATATTTCAACCAATTACAGCACCAATTCAGTCACTGCCAGCCCACCAAATAAATCAAGGGGTTACATGAAAACGTAGCCCCTTTTTCTTTGGTAGTGACACTAAAATGGATGTAGTGTGAAGAATAATCCCGTTTACTCAATCAAT‑AATACATATTGTTTCAATCTACGTTATTATCTCTTTGTAAAAATTGCCATTTATTAATCATTG  >  NC_000913/2783379‑2783643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑