Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,801,641	T→C	R98R (CGT→CGC)	nrdE →	ribonucleoside‑diphosphate reductase 2 subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,801,641	0	T	C	100.0%	242.3 / NA	67	R98R (CGT→CGC)	nrdE	ribonucleoside‑diphosphate reductase 2 subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/33);  total (34/33)

GACCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACG  >  NC_000913/2801509‑2801776                                                                                                                                     |                                                                                                                                        gACCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGa                                                                                                                                  <  2:66173/140‑1 (MQ=255)  aCCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGAc                                                                                                                                 <  2:864724/140‑1 (MQ=255)      tCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTc                                                                                                                             >  2:54116/1‑140 (MQ=255)      tCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTc                                                                                                                             >  2:1056412/1‑140 (MQ=255)       cAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTA‑CCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCt                                                                                                                           <  1:1192643/140‑1 (MQ=255)        aGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCt                                                                                                                           >  2:1157462/1‑140 (MQ=255)        aGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCt                                                                                                                           >  1:174999/1‑140 (MQ=255)        aGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCt                                                                                                                           <  2:274314/140‑1 (MQ=255)        aGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCt                                                                                                                           <  1:945666/140‑1 (MQ=255)        aGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCt                                                                                                                           <  1:1056412/140‑1 (MQ=255)          tAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCGCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCg                                                                                                                         <  1:445958/140‑1 (MQ=255)            gCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCggg                                                                                                                       >  1:593469/1‑140 (MQ=255)             ccAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCgggg                                                                                                                      >  1:496604/1‑140 (MQ=255)                 cagcagcGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATg                                                                                                                  >  2:137356/1‑140 (MQ=255)                 cagcagcGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATg                                                                                                                  >  1:484081/1‑140 (MQ=255)                  agcagcGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATgg                                                                                                                 <  2:211632/140‑1 (MQ=255)                  agcagcGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATgg                                                                                                                 >  1:522196/1‑140 (MQ=255)                     agcGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAg                                                                                                              >  2:382181/1‑140 (MQ=255)                      gcgcCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGt                                                                                                             >  2:970880/1‑140 (MQ=255)                       cgcCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGtt                                                                                                            >  2:588983/1‑140 (MQ=255)                           tGAACTGGCTAGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTac                                                                                                        <  2:797587/140‑1 (MQ=255)                            gAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTCAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTaca                                                                                                       <  1:367269/140‑1 (MQ=255)                             aaCTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTacac                                                                                                      <  1:30835/140‑1 (MQ=255)                              aCTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACAcc                                                                                                     <  2:522196/140‑1 (MQ=255)                                   ctggTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCta                                                                                                >  2:991317/1‑140 (MQ=255)                                   ctggTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCta                                                                                                <  1:382181/140‑1 (MQ=255)                                    tggTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCtat                                                                                               <  2:891322/140‑1 (MQ=255)                                    tggTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCtat                                                                                               <  1:991317/140‑1 (MQ=255)                                       tCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTac                                                                                                        >  1:1091893/1‑128 (MQ=255)                                       tCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTATTTACCCACGCACACACCAGCGGTTTTCGCTTTCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACg                                                                                            >  2:1147738/1‑140 (MQ=255)                                       tCAACGAAGGTTACCATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCCCTTCCAGACATTCCTCGGGGCATGGAAGTTTTac                                                                                                        <  2:1091893/128‑1 (MQ=255)                                                gTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACa                                                                                   >  2:1049012/1‑140 (MQ=255)                                                  tACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACAtt                                                                                 <  2:96251/140‑1 (MQ=255)                                                   aCTGTGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTc                                                                                <  2:174999/140‑1 (MQ=255)                                                         atgaAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGt                                                                          >  2:903224/1‑140 (MQ=255)                                                         atgaAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGt                                                                          <  1:1180117/140‑1 (MQ=255)                                                          tgaAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTa                                                                         >  1:805095/1‑140 (MQ=255)                                                            aaaGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTaaa                                                                       >  2:1176764/1‑140 (MQ=255)                                                             aaGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAAc                                                                      >  2:1134682/1‑140 (MQ=255)                                                              aGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACg                                                                     >  1:57124/1‑140 (MQ=255)                                                              aGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACg                                                                     >  1:862238/1‑140 (MQ=255)                                                              aGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACg                                                                     >  1:393893/1‑140 (MQ=255)                                                                  ttCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTAt                                                                 >  1:937672/1‑140 (MQ=255)                                                                     ttAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTg                                                              <  2:862238/140‑1 (MQ=255)                                                                       aaTCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGa                                                            <  2:57124/140‑1 (MQ=255)                                                                          cGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGa                                                         >  1:168132/1‑140 (MQ=255)                                                                             tACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGAttt                                                      >  1:396796/1‑140 (MQ=255)                                                                               ctctCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTg                                                    >  1:1008622/1‑140 (MQ=255)                                                                                tctcGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGc                                                   <  1:1134682/140‑1 (MQ=255)                                                                                   cgcgACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGa                                                <  1:588983/140‑1 (MQ=255)                                                                                    gcgACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGAt                                               <  2:393893/140‑1 (MQ=255)                                                                                    gcgACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGAt                                               <  1:1049012/140‑1 (MQ=255)                                                                                    gcgACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGAt                                               <  1:1157462/140‑1 (MQ=255)                                                                                            gTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAAc                                       <  2:484081/140‑1 (MQ=255)                                                                                                  aCGCTGTTTACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGAtggt                                 >  1:1177561/1‑140 (MQ=255)                                                                                                        tttACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCt                           <  1:903224/140‑1 (MQ=255)                                                                                                         ttACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTg                          >  2:1093911/1‑140 (MQ=255)                                                                                                          tACCCACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGa                         >  1:632988/1‑140 (MQ=255)                                                                                                            cccACGCACACACCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACg                       <  2:593469/140‑1 (MQ=255)                                                                                                                  cacacaCCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATAGAGTAACGATGGTGGCGCTGACGCTGGca                 >  1:1210565/1‑140 (MQ=255)                                                                                                                   acacacCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGcac                <  1:1093911/140‑1 (MQ=255)                                                                                                                    cacacCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGGTGGTGGCGCTGACGCTGGcaca               <  1:54116/140‑1 (MQ=255)                                                                                                                       acCAGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAgg            <  1:1147738/140‑1 (MQ=255)                                                                                                                          aGCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGa         >  2:812859/1‑140 (MQ=255)                                                                                                                           gCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGAt        <  2:1008622/140‑1 (MQ=255)                                                                                                                           gCGGTTTTCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGAt        >  1:757691/1‑140 (MQ=255)                                                                                                                                 ttCGCTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACg  >  1:391665/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        GACCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTACCCACGCACACACCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACG  >  NC_000913/2801509‑2801776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑