Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	161,235	C→T	W84* (TGG→TGA)	sfsA ←	putative DNA‑binding transcriptional regulator of maltose metabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	161,235	0	C	T	100.0%	210.4 / NA	59	W84* (TGG→TGA)	sfsA	putative DNA‑binding transcriptional regulator of maltose metabolism
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/28);  total (31/28)

ACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGC  >  NC_000913/161100‑161369                                                                                                                                        |                                                                                                                                       aCGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCaaa                                                                                                                                    >  1:831973/1‑140 (MQ=255)      aaTCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCgtg                                                                                                                               >  2:778424/1‑140 (MQ=255)       aTCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCgtgt                                                                                                                              <  2:1225144/140‑1 (MQ=255)           gCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGAc                                                                                                                          <  1:1125050/140‑1 (MQ=255)              tGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCa                                                                                                                       <  1:506198/140‑1 (MQ=255)                cAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCaaa                                                                                                                     >  2:354563/1‑140 (MQ=255)                       aaGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATgc                                                                                                              <  2:1090822/140‑1 (MQ=255)                        aGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATgcg                                                                                                             <  2:831973/140‑1 (MQ=255)                         gTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATgcgc                                                                                                            <  1:217602/140‑1 (MQ=255)                             aTACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGct                                                                                                        <  1:778424/140‑1 (MQ=255)                                 ggCTGCGTTCTGCGCCGTGTTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTgg                                                                                                    >  2:460278/1‑140 (MQ=255)                                  gCTGCGTTCTGCGCCGTGTTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGc                                                                                                   <  1:460278/140‑1 (MQ=255)                                   cTGCGTTCTGCGCCGTATTTTACATCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCt                                                                                                  >  2:162543/1‑140 (MQ=255)                                    tGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGTCAGTTCTGATATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCtt                                                                                                 <  1:1078358/140‑1 (MQ=255)                                    tGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCtt                                                                                                 <  1:615494/140‑1 (MQ=255)                                    tGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCtt                                                                                                 <  1:1208551/140‑1 (MQ=255)                                    tGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCtt                                                                                                 >  2:968975/1‑140 (MQ=255)                                    tGCGTTCTGCGCCGTATTTTACTTCGCCTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCtt                                                                                                 >  2:951955/1‑140 (MQ=255)                                         tCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGt                                                                                            <  1:354563/140‑1 (MQ=255)                                         tCTGCGCCGTATTTTACATCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGt                                                                                            <  1:162543/140‑1 (MQ=255)                                           tGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTa                                                                                          <  1:1058215/140‑1 (MQ=255)                                                         cTTCGCCTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGgtgtgt                                                                            <  1:951955/140‑1 (MQ=255)                                                            cGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTggg                                                                         >  1:201161/1‑140 (MQ=255)                                                            cGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTggg                                                                         >  2:31486/1‑140 (MQ=255)                                                            cGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTggg                                                                         >  1:789771/1‑140 (MQ=255)                                                              cTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTa                                                                       >  1:1209024/1‑140 (MQ=255)                                                                 ttCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAg                                                                                  >  1:1123785/1‑126 (MQ=255)                                                                 ttCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAg                                                                                  <  2:1123785/126‑1 (MQ=255)                                                                       gAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGttt                                                              <  1:901606/140‑1 (MQ=255)                                                                        aGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTg                                                             >  2:1216451/1‑140 (MQ=255)                                                                           tataGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGgtg                                                          >  1:1129819/1‑140 (MQ=255)                                                                                    aCAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGaa                                                 >  2:998572/1‑140 (MQ=255)                                                                                     cAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAg                                                >  2:715503/1‑140 (MQ=255)                                                                                        ttCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGgtg                                                          <  1:1137545/127‑1 (MQ=255)                                                                                        ttCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGgtg                                                          >  2:1137545/1‑127 (MQ=255)                                                                                        ttCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCg                                             <  1:850891/140‑1 (MQ=255)                                                                                               aTTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCa                                      <  2:1209024/140‑1 (MQ=255)                                                                                                 tGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGa                                    <  2:1145296/140‑1 (MQ=255)                                                                                                  gATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGAc                                   >  1:1048394/1‑140 (MQ=255)                                                                                                     tCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGt                                <  2:1129819/140‑1 (MQ=255)                                                                                                           aGGATAGCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGcc                          >  1:640196/1‑140 (MQ=255)                                                                                                                 gCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGt                    >  2:1162011/1‑140 (MQ=255)                                                                                                                 gCCTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGt                    >  2:744594/1‑140 (MQ=255)                                                                                                                  ccTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGtt                   >  2:1155810/1‑140 (MQ=255)                                                                                                                  ccTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGtt                   >  1:50553/1‑140 (MQ=255)                                                                                                                  ccTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGtt                   >  1:594650/1‑140 (MQ=255)                                                                                                                  ccTCTTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGtt                   >  1:721184/1‑140 (MQ=255)                                                                                                                   ctctTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTg                  <  2:610841/140‑1 (MQ=255)                                                                                                                   ctctTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTg                  <  1:295250/140‑1 (MQ=255)                                                                                                                     ctTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGca                <  2:50553/140‑1 (MQ=255)                                                                                                                     ctTTCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGca                <  1:1162011/140‑1 (MQ=255)                                                                                                                       ttCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGcaca              >  2:708735/1‑140 (MQ=255)                                                                                                                        tCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGca                >  1:1042759/1‑137 (MQ=255)                                                                                                                        tCGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGca                <  2:1042759/137‑1 (MQ=255)                                                                                                                         cGTCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAAc            <  2:640196/140‑1 (MQ=255)                                                                                                                           tCAACCTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCg          >  1:1153126/1‑140 (MQ=255)                                                                                                                               ccTGTTAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCa      >  1:416862/1‑140 (MQ=255)                                                                                                                                   ttAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGc  >  1:518206/1‑140 (MQ=255)                                                                                                                                   ttAGCTCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGc  >  2:521089/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       ACGCGAATCCGCCTGCAACATAAAGTCAATACGGCTGCGTTCTGCGCCGTATTTTACTTCGCTTTTCAGCGAGCTATAGCCTGACAGTTCTGAAATTGATTCATTAAGGATAGCCTCTTTCGTCAACCTGTTAGCCCAAAGCGTGTTGACGCAAATAAATGCGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGC  >  NC_000913/161100‑161369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑